MO Xili,JIANG Xuanli.Mechanism of BTH Inducing Resistance Against Powdery Mildew of Pepper[J].Northern Horticulture,2016,40(16):113-118.[doi:10.11937/bfyy.201616030]
苯丙噻二唑诱导辣椒抗白粉病机制研究
- Title:
- Mechanism of BTH Inducing Resistance Against Powdery Mildew of Pepper
- 关键词:
- 苯丙噻二唑(BTH); 辣椒; 防御酶
- Keywords:
- BTH; pepper; defense enzyme
- 文献标志码:
- A
- 摘要:
- 以花溪辣椒、遵义辣椒为试材，辣椒白粉病菌为供试菌，苯丙噻二唑(BTH)为诱导剂，采用喷施接种法，研究了 BTH诱导辣椒对白粉病产生抗性的作用机制。结果表明：不同浓度BTH均可以诱导辣椒对白粉病产生抗性，浓度为0.20 mmol?L-1时诱导效果最佳，防治效果为81.82%；BTH-接菌处理的辣椒叶片过氧化物酶(POD)、多酚氧化酶(PPO)、苯丙氨酸解氨酶(PAL)活性明显增加；BTH-接菌、BTH-未接菌和CK-接菌处理都能诱导辣椒叶片几丁质酶和β-1,3-葡聚糖酶活性的升高，但CK-接菌处理增加幅度较小。BTH不影响白粉病菌孢子萌发和附着胞子的产生，却能有效抑制菌丝的生长，表现为菌丝生长缓慢和菌丝的侧枝数减少。通过扫描电镜观察，发现CK-接菌处理在24 h后，辣椒叶片保护层开始被溶解，BTH-接菌处理的辣椒在接菌48 h后才出现该现象，说明BTH能产生阻碍或延迟菌丝破坏的保护层物质。
- Abstract:
- Taking Huaxi pepper and Zunyi pepper as materials，powdery mildew as test fungi，BHT as inducer，using spraying method，the mechanism of inducing resistance against powdery mildew of pepper was studied.The results showed that the resistance of pepper to powdery mildew was induced by BTH at different concentrations，and the best control effect was 81.82% after 0.20 mmol?L-1 BTH spraying treatment.The activities of POD,PPO and PAL were raised obviously after treated by BTH-Leveillula taurica inoculation.The chitinase and β-1,3-glucanase activities of pepper increased after BTH-Leveillula taurica treatment and BTH-water treatment,and which were better than the CK-Leveillula taurica treatment.BTH would not affect the spore germination and the appressorium generation，but it could effectively control the expansion of hypha，such as the hypha slow expansion and collateral reducing.Through the scanning electron microscopy (sem)，the result showed that the blade coating of pepper begins to be dissolved after 24 hours by CK-Leveillula taurica treatment.But in BTH-Leveillula taurica treatment，the blade coating appear the phenomenon after 48 hours.That the pepper was induced to produce chemicals which could put off or delay hyphae destroy the protective layer of pepper.
参考文献/References:
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备注/Memo
第一作者简介：莫熙礼（1982-），男，广西梧州人，硕士，讲师，现主要从事植物病虫害防治教学及科研等工作。E-mail:moxili1982@163.com.基金项目：黔西南州科技局计划资助项目（2015-1-14）。