[1]颜秀娟,何鑫,王学梅,等.宁夏羊角椒雄性不育基因ISSR的分子标记[J].北方园艺,2015,39(17):82-84.[doi:10.11937/bfyy.201517021]
　YAN Xiujuan,HE Xin,WANG Xuemei,et al.ISSR Marker of Male Sterility in Pepper of Ningxia[J].Northern Horticulture,2015,39(17):82-84.[doi:10.11937/bfyy.201517021]
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宁夏羊角椒雄性不育基因ISSR的分子标记

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第39卷 期数: 2015年17 页码: 82-84 栏目: 出版日期: 2015-09-15
Title:
ISSR Marker of Male Sterility in Pepper of Ningxia
作者:
颜秀娟1; 何鑫2; 王学梅1; 高晶霞1
（1.宁夏农林科学院 种质资源研究所，宁夏 银川 750002；2.宁夏森林病虫防治检疫总站，宁夏 银川 750001）
Author(s):
YAN Xiujuan1; HE Xin2; WANG Xuemei1; GAO Jingxia1
（1.Institute of Germplasm Resources，Ningxia Academy of Agriculture and Forestry Sciences，Yinchuan,Ningxia 750002;2.General Station of Forest Pest Management and Quarantine of Ningxia，Yinchuan,Ningxia 750001）
关键词:
宁夏羊角椒; 雄性不育; ISSR标记
Keywords:
pepper of Ningxia; male sterility; ISSR marker
DOI:
10.11937/bfyy.201517021
文献标志码:
A
摘要:
以宁夏羊角椒雄性不育系为试材，应用ISSR技术，对其不育系及其相应的可育系基因组DNA进行了比较分析，从50条随机引物中筛选了35条引物在两系之中都获得了ISSR-PCR扩增产物。结果表明：在不育系中获得4条稳定扩增的特异片段ISSR-10850、ISSR-13850、ISSR-14800和ISSR-15180，初步认定这可能是导致宁夏羊角椒雄性不育系花粉败育的不育基因相关的分子标记。
Abstract:
Taking male sterility of pepper in Ningxia as material,the comparative analysis of genomic DNA between male sterile line NYB and its maintainer were made by means of ISSR technique.35 primers were amplified bands in two lines from 50 random primers among which the specific fragment ISSR-10850，ISSR-13850，ISSR-14800 and ISSR-15180 of NYB pepper was found.The results showed that ISSR-10850、ISSR-13850、ISSR-14800 and ISSR-15180 were likely linked with male sterile gene.

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备注/Memo

第一作者简介：颜秀娟（1981-），女，吉林长春人，硕士，助理研究员，研究方向为辣椒育种与栽培。E-mail：aixin0516@163.com.基金项目：宁夏农林科学院自主研发资助项目(NKYG14-14,NKYG-15-01）。

更新日期/Last Update: 2015-10-10