ZOU Chun-lei,LIU Chang-yuan,WANG Li-ping,et al.Relationship Between the Activity of Four Defense Enzymes inLeaf and Phytophthora resistant of Pepper[J].Northern Horticulture,2015,39(12):115-118.[doi:10.11937/bfyy.201512029]
四种叶片防御酶活性与辣椒对疫病抗性的关系
- Title:
- Relationship Between the Activity of Four Defense Enzymes inLeaf and Phytophthora resistant of Pepper
- 文章编号:
- 1001-0009（2015）12-0110-05
- Keywords:
- pepper; Phytophthora capsici; defense enzymes; disease resistance
- 分类号:
- S 436.341.1
- 文献标志码:
- A
- 摘要:
- 以对辣椒疫霉菌3号生理小种具有不同抗性的辣椒近等基因系为试材，测定了不同抗性品系接菌后β-1,3-葡聚糖酶、几丁质酶、PAL、POD活性的变化情况，研究了上述4种叶片防御酶活性与辣椒对疫病抗性的关系。结果表明：辣椒疫霉菌能诱导辣椒叶片中上述4种防御酶的活性增强，但4种酶在积累速度和幅度上抗病品系和感病品系有显著的差异。接菌后高抗品系β-1,3-葡聚糖酶、几丁质酶、PAL、POD活性达到峰值时酶活变化率分别是164.4%、99.1%、173.7%、107.6%；而感病品系的酶活变化率分别是91.8%、48.1%、93.1%、64.0%，与感病品系相比，高抗品系的4种酶活性不仅升高的速度快、幅度大，且高活性维持时间长，中抗品系的4种酶活性介于二者之间。在抗病品系中，β-1,3-葡聚糖酶的酶活变化率是几丁质酶的1.66倍，PAL的酶活变化率是POD的1.61倍。在离体培养条件下观察了β-1,3-葡聚糖酶和几丁质酶粗酶液对辣椒疫霉菌的菌丝生长和孢子囊形成的抑制作用，与对照相比，β-1,3-葡聚糖酶粗酶液抑制作用明显，而几丁质酶粗酶液抑制作用不明显。
- Abstract:
- Taking three near isogenic lines with different phytophthora resistance as the experimental material,the activities of four enzymes(β-1，3-glucanase,chitinase,PAL,POD) were detected after induced by race 3 of Phytophthora capsici. The relationship between the activity of four defense enzymes in leaf and phytophthoraresistant of pepper was studied.The results showed that the activities of four defense enzymes in pepper’s leaves were increased by infection of Phytophthora capsici, the rate of enzyme activity for β-1,3-glacanase，chitinase，PAL，POD in resistant lines after infection of Phytophthora capsici was 164.4%,99.1%,173.7%,107.6%；but in susceptible lines was 91.8%,48.1%,93.1%,64.0%,the increase in activities was faster and higher，and lasted longer in a resistant lines than that in susceptible lines.The activities of enzymes in mid resistant lines were between two of above.The results suggested that β-1,3-glacanase,chitinase,PAL and POD could be induced by Phytophthora capsici and the relationship between the activities of the four enzymes and the resistance of pepper was positive.In the resistant line,the rate of enzyme activity for β-1,3-glacanase was 1.66 times higher than chitinase;PAL was 1.61 times higher than POD.The inhibition of β-1,3-glacanase and chitinase to mycelia growth and sporangia formation were observed.β-1,3-glacanase was significantly higher than that of control，chitinase showed no clear inhibition to Phytophthora capsici.
参考文献/References:
[1] van Loon L C,van Strien E A.The families of pathogenesis related proteins, their activities,and comparative analysis of PR1 type protein[J].Physiology Molecular Plant Pathology’1999,55:85-97.[2] Mauch F,Mauchmani B,Boller T.Antifungal hydrolases in pea tissueⅡ.Inhibition of fungal growth by combinations of chitinase and β-1,3-glucanase[J].Plant Physiology,1988,88:936-942. [3]Cachinero J M,Cabello F,Jorrin J,et al.Induction of different chitinase and β-1,3-glucanase isoenzymes in sunflower(Helianthus annuus L.)seedlings in response to infection by Plasmopara halstedii[J].European Journal of Plant Pathology,1996,102(4):401-405.[4]Egea C,Ahmed A S,Candela M,et al.Elicitation of peroxidase activity and lignin biosynthesis in pepper suspension cells by Phytophthora capsici[J].Journal of Plant Physiology,2001,158:151-158.[5] Jung W J,Jin Y L,Kim Y C,et al.Inoculation of Paenibacillus illinoisensis alleviates root mortality activates of lignifications related enzymes and induction of the isozymes in pepper plantsinfected by Phytophthora capsici[J].Biological Control,2004,30:645-652.[6]Mandal S,Mallick N,Mitra A.Salicylic acidinduced resistance to Fusarium oxysporum f.sp.lycopersici in tomato[J].Plant Physiology and Biochemistry,2009,47(7):642-649.[7] 周宝利,陈志霞,杜亮,等.不同品种茄子抗黄萎病特性与生理生化指标及根际微环境指标的相关性分析[J].沈阳农业大学学报,2013,44(2):136-142.[8] 姜飞,刘业霞,刘伟,等.嫁接辣椒根腐病抗性及其与苯丙烷类物质代谢的关系[J].中国蔬菜,2010(8):46-52.[9]张里,刘长远,赵奎华,等.辽宁省辣椒疫霉菌生理小种鉴定[J].沈阳农业大学学报,2014,45(1):95-98.[10]NY/T 2060.1-2011.辣椒抗病性鉴定技术规程 第1部分:辣椒抗疫病鉴定技术规程[S].2011.[11]Steiner O，Boslandp W.Recombinant inbred line differential identifies race specific resistance to phytophthora root rot in Capsicum annuum[J].Genetics and Resistanc,2008,98(8):867-870.[12]Bosland P W,Lindsey D L.A seedling screen for Phytophthora root rot of pepper,Capsicum annuum[J].Plant Dis,1991,75:1048-1050.[13]左豫虎,康振生,杨传平,等.β-1,3-葡聚糖酶和几丁质酶活性与大豆对疫霉根腐病抗性的关系[J].植物病理学报,2009,39(6):600-607.[14]郝建军,刘延吉.植物生理学实验技术[M].沈阳:辽宁科学技术出版社,2001.[15]张立军,樊金娟.植物生理学实验教程[M].北京:中国农业大学出版社,2007.[16]史娟,李建设.枯萎病菌诱导的几丁质酶和β-1,3-葡聚糖酶与寄主抗病性的关系[J].农业科学研究,2006(3):24-26.[17]陈坚,李冠,王锐萍.新疆甜瓜疫霉菌毒素对新疆甜瓜黄化苗中β-1, 3-葡聚糖酶和几丁酶活性的诱导[J].植物生理学通讯,1998,34(4):256-258.[18]史娟,胡景江,王红玲,等.霜霉病菌对葡萄细胞壁水解酶的诱导作用与寄主抗病性的关系[J].西北林学院学报,2002,17(1):42-44.[19]Jebakumar R S,Anandaraj M,Sarma Y R.Induction of PR proteins and defense related enzymes in black pepper due to inoculation with Phytophthora capsici[J].Phytopathology,2002,54:23-28.[20]Randall T A,Dwyer R A,Huitema E,et al.Large scale gene discovery in the heoomycete Phytophthora infestans reveals likely components of phytopathogenicity shared with true fungi[J].Moleclar Plant Microbe Interactions,2005,18(3):229-243.[21]王茹华,周宝利,张启发,等.茄子/番茄嫁接植株的生理特性及其对黄萎病的抗性[J].植物生理学通讯,2003,39(4):330-332.[22]刘业霞,付玲,艾希珍,等.嫁接对辣椒次生代谢的影响及其与青枯病抗性的关系[J].中国农业科学,2013,46(14):2963-2969.[23]Alcazar M D,Egea C,Espin A,et al.Peroxidase isoenzyme in the defense response of Capsicum annum to Phytophtora capsici[J].Physiologia Plantarum,1995,94:736-742.
相似文献/References:
[1]胡凤霞,唐艳领,刘金,等.辣椒砧木的筛选及其耐盐性研究[J].北方园艺,2013,37(04):24.
[2]陈 潇,盛萍萍,刘润进,等.西瓜与辣椒间作体系接种AM真菌对连作西瓜植株[J].北方园艺,2014,38(12):6.
CHEN Xiao,SHENG Ping-ping,LIU Run-jin,et al.Effect of Intercropping System of Pepper-Watermelon Crops Inoculating Arbuscular Mycorrhizal Fungus on Growth and Defence Enzymes Activity of the Continuous Cropping Watermelon[J].Northern Horticulture,2014,38(12):6.
[3]张婷玉,林 多,杨延杰.辣椒根系分泌物的收集方法研究[J].北方园艺,2014,38(12):14.
ZHANG Ting-yu,LIN Duo,YANG Yan-jie.Study on Collection Method of Root Exudates on Pepper[J].Northern Horticulture,2014,38(12):14.
[4]叶 林,李春江,张光弟,等.不同育苗穴数、基质配比和施肥量对温室辣椒幼苗生长及其秧苗质量的影响[J].北方园艺,2014,38(13):50.
YE Lin,LI Chun-jiang,ZHANG Guang-di,et al.Effects of Different Seedling Number of Holes，Substrates and Fertilization on the Growth and Quality of Greenhouse Pepper Seedlings[J].Northern Horticulture,2014,38(12):50.
[5]孔小平,苗增建.等离子体处理辣椒种子对其性状及产量的影响[J].北方园艺,2013,37(13):34.
KONG Xiao-ping,MIAO Zeng-jian.Effect of Plasma Treatment on Characters and Output of Pepper Seeds[J].Northern Horticulture,2013,37(12):34.
[6]高万里.白城地区辣椒产业发展对策研究[J].北方园艺,2014,38(04):159.
GAO Wan-li.Study on the Development Countermeasure of Pepper Industry in Baicheng Area[J].Northern Horticulture,2014,38(12):159.
[7]岳振平.不同种植密度对早春大棚辣椒产量和产值的影响[J].北方园艺,2014,38(05):51.
YUE Zhen-ping.Effect of Different Planting Densty on Fruit Yield and Output Value of Pepper in Early Spring[J].Northern Horticulture,2014,38(12):51.
[8]郭亚华,谢立波,王雪,等.辣椒经地面模拟诱变后的RAPD鉴定[J].北方园艺,2014,38(05):95.
GUO Ya-hua,XIE Li-bo,WANG Xue,et al.The RAPD Detection of Capsicum annuum L. After Ground Simulation[J].Northern Horticulture,2014,38(12):95.
[9]杨飞,高艳明,李建设.宁夏设施沙培辣椒引种试验[J].北方园艺,2013,37(10):44.
YANG Fei,GAO Yan-ming,LI Jian-she.Introduction Test of Sand Culture Facilities Pepper in Ningxia[J].Northern Horticulture,2013,37(12):44.
[10]马寿宾,孙艳,王琛,等.分子标记辅助选择辣椒抗疫病新种质研究[J].北方园艺,2013,37(10):107.
MA Shou-bin,SUN Yan,WANG Chen,et al.Molecular Marker Assisted Selection of Pepper Materials with Resistance to Phytophthora Blight[J].Northern Horticulture,2013,37(12):107.
备注/Memo
第一作者简介：邹春蕾（1981-），女，硕士，助理研究员，现主要从事辣椒抗病育种等研究工作。E-mail：277350850@qq.com.责任作者：刘长远（1962-），男，博士，研究员，博士生导师，现主要从事园艺作物病害防治等研究工作。基金项目：辽宁省农业攻关计划资助项目（2014215016；2010215003）；辽宁省农业领域青年科技创新人才培养计划资助项目（2014031）。