[1]汪琛颖,张 坤,梁 芳,等.蕙兰(Cymbidium faberi Rolfe)基因组大小测定[J].北方园艺,2015,39(11):86-90.[doi:10.11937/bfyy.201511021]
　WANG Chen-ying,ZHANG Kun,LIANG Fang,et al.Estimation of Genome Size of Cymbidium faberi Rolfe[J].Northern Horticulture,2015,39(11):86-90.[doi:10.11937/bfyy.201511021]
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蕙兰(Cymbidium faberi Rolfe)基因组大小测定

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第39卷 期数: 2015年11 页码: 86-90 栏目: 出版日期: 2015-06-15
Title:
Estimation of Genome Size of Cymbidium faberi Rolfe
文章编号:
1001-0009(2015）11-0086-05
作者:
汪琛颖1; 2; 张 坤3; 梁 芳4; 崔 波4; 马润林5
1.郑州师范学院 生命科学学院，河南 郑州 450044；2.郑州师范学院 分子生物学实验室，河南 郑州 450044；3.黑龙江大学 生命科学学院，黑龙江 哈尔滨150080；4.郑州师范学院 生物工程研究所，河南 郑州 450044；5.中国科学院 遗传与发育生物学研究所，北京 100101
Author(s):
WANG Chen-ying1; 2; ZHANG Kun3; LIANG Fang4; CUI Bo4; MA Run-lin5
1.College of Life Science，Zhengzhou Normal University，Zhengzhou，Henan 450044；2.Molecular Biology Laboratory，Zhengzhou Normal University，Zhengzhou,Henan 450044；3.College of Life Science，Heilongjiang University，Harbin，Heilongjiang 150080；4.Institute of Bioengineering，Zhengzhou Normal University，Zhengzhou,Henan 450044；5.Institute of Genetics and Development Biology,Chinese Academy of Sciences,Beijing 100101
关键词:
蕙兰; 基因组大小; 流式细胞术; 铁皮石斛
Keywords:
Cymbidium faberi; genome size; flow cytometry; Dendrobium officinal
分类号:
S 682.31
DOI:
10.11937/bfyy.201511021
文献标志码:
A
摘要:
以蕙兰为试材，采用流式细胞分析技术，以铁皮石斛为外参，对其基因组大小进行估算。结果表明：蕙兰的基因组大小为(4.39±0.12)Gb，即2C DNA含量为8.98 pg。该研究探索了用流式细胞仪分析蕙兰基因组大小的方法，所得数据为蕙兰基因组测序提供了必要的基础。
Abstract:
aking Cymbidium faberi as material，using D.of ficinale Kimura et Migo as an external reference,flow cytometry measurements were performed when the genome size of Cymbidium faberi was estimated.The results showed that,the estimated genome size of C.faberi was (4.39±0.12）Gb,or the content of 2C DNA was about 8.98 pg.The present study described a modified method of genome size analysis by using flow cytometry in C.faberi.The data available of DNA content obtained in this study was helpful to the future genome sequencing research.

参考文献/References:

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备注/Memo

第一作者简介：汪琛颖(1968-），女，博士，教授，现主要从事植物分子系统发育等研究工作。E-mail：cywang2013@hotmail.com.基金项目：河南省科技计划资助项目(132300410358)。

更新日期/Last Update: 2015-08-06