LIU Bu-cang,ZHANG Ai-xia,LI Yu-shun,et al.Establishment of High Frequency Regeneration System of Portulace oleracea L.and Determination of Phosphinothricin Sensitivity[J].Northern Horticulture,2015,39(07):98-103.[doi:10.11937/bfyy.201507029]
马齿苋高频再生体系的建立及对草丁膦敏感性测定
- Title:
- Establishment of High Frequency Regeneration System of Portulace oleracea L.and Determination of Phosphinothricin Sensitivity
- 文献标志码:
- A
- 摘要:
- 以马齿苋子叶和下胚轴为外植体，研究了外源激素对其不定芽的诱导及不同浓度6-BA、NAA、GA3对不定芽伸长的影响，并测定了马齿苋子叶和下胚轴对草丁膦的敏感性。结果表明：MS+2.0 mg/L 6-BA+100 mg/L水解酪蛋白为下胚轴不定芽再生最适培养基，不定芽再生率达 96.65%；MS+3.0 mg/L 6-BA+100 mg/L水解酪蛋白为子叶不定芽再生最适培养基，不定芽再生率达100%；最佳的不定芽继代培养的培养基为MS+0.5 mg/L 6-BA+0.05 mg/L NAA+0.2 mg/L GA3；生根培养基为MS+2.0 mg/L IBA，生根率达100%；以子叶和下胚轴为材料研究其对草丁膦的敏感性，发现子叶对草丁膦更敏感，0.050 mg/L 草丁膦对子叶的致死率达到89.0%；对下胚轴的致死率为71.5%。该研究建立了马齿苋植株高频再生体系，并确定了其对草丁膦的敏感性，可为该品种的基因工程研究提供必要的理论和实践基础。
- Abstract:
- Taking Portulaca oleracea L.cotyledon and hypocotyl as explants，the effect of exogenous hormone on the inducement of adventitious buds，and different concentration of 6-BA，NAA，GA3 on the elongation of adventitious buds were studied,the sensitivity of phosphinothricin of Portulaca oleracea L.cotyledon and hypocotyl were determined.The results showed that the optimum induction mediun of hypocotyl adventitious buds was MS+2.0 mg/L 6-BA+100 mg/L CH (casein acid hydrolysate）and the average induction rate was 96.65%；the optimum mediun of cotyledon adventitious buds induction was MS+3.0 mg/L 6-BA+100 mg/L CH(casein acid hydrolysate）and the average induction rate was 100%;the optimum shoot growth medium was MS+0.5 mg/L 6-BA+0.05 mg/L NAA+0.2 mg/L GA3；the rooting medium was MS+2.0 mg/L IBA with rooting rate 100%.Determination of the phosphinothricin sensitivity showed that compared to hypocotyl，cotyledon was more sensitive to phosphinothricin.At 0.050 mg/L PPT，the fatality rate was 89.0% and 71.5% in cotyledon and hypocotyl respectively.This study established the high frequency regeneration system of Portulace oleracea L.and determined its sensitivity to phosphinothricin.Results from this study would help the research of Portulaca oleracea L. in genetic engineering.
参考文献/References:
[1]王红艳,王松丽.野生蔬菜K马齿苋的研究进展[J].中国农学通报,2004,20(2):31-36. [2]李利峰.马齿苋——野生蔬菜中的保健珍品[J].辽宁农业科学,1997(4):56. [3]王书荣.菜中之鱼—马齿苋[J].中国蔬菜,1998(1):51-52. [4]杨春玲,孙克威,王永华,等.马齿苋营养价值及其设施栽培技术[J].辽宁农业职业技术学报,2007(1):21-22. [5]闰明,乔秀红.马齿苋的经济价值及开发利用[J].农牧产品开发,2000(5):25-27. [6]谭丽霞,周求良.马齿苋的营养成分分析及其开发利用[J].中国野生植物资源,2008(2):49-50. [7]丁怀伟,姚佳琪,宋少江.马齿苋的化学成分和药理活性研究进展[J].沈阳药科大学学报,2008(10):831-837. [8]曹涤环.浑身是宝的马齿苋[J].中国土特产,1996(3):34-35. [9]张兆洪,孙美荣.保健植物马齿苋[J].生物学杂志,1991(1):26-27. [10]翟玮玮,顾立众.马齿苋功能因子及保健食品的开发[J].食品科技,2003(2):20-24. [11]刘鹏岩,郭志峰,安秋荣,等.马齿苋及其籽中脂肪酸的比较研究[J].分析测试学报,1995,14(5)：70-72. [12]Burr G O,Burr M M.On the nature and role of the fatty acids essential innutrition[J].J Biol Chem,1999，86：587-621. [13]Long J J,Fu Y J.Ultrasound-assisted extraction of flaxseed oil using immobilized enzymes[J].Bioresource Technology，2011,9(102)：991-996. [14]李焘,王喆之.马齿苋组织培养及植株再生系统的建立[J].陕西师范大学学报,2003,31(2):95-98. [15]刘红梅,蒋继志,宫卫波,等.马齿苋组织培养的优化[J].华北农学报,2006,21(Z1):63-66. [16]王红艳,王松丽,黄群策.野生蔬菜马齿苋的研究进展[J].中国农学通报,2004,20(2):31-38. [17]邱宁宏,周丹丽,韩露.马齿苋的组织培养与快速繁殖[J].中国野生植物资源,2003,22(2):58-59. [18]黄群策,贾宏汝,王红艳.马齿苋植物的无性系及其植株再生体系[J].北方园艺,2007(2):142-145. [19]黄红梅,彭超宇,刘树玲,等.刘清波马齿苋离体培养再生体系的建立[J].中国农学通报，2011,27(22)：136-141. [20]李贞霞,董卫华,陈碧华.马齿苋组织培养研究[J].贵州农业科学，2008,36(3):16-17. [21]黎垣庆,刘刚,严文贵,等.除草剂(草丁膦)抗性基因的遗传与利用[J].植物学报,1999,41(12):1348-1350. [22]李永光,黄文佳,周失，等.大豆对草丁膦敏感性研究[J].大豆科学,2011,30(5):749-756. [23]韦银凤,王瑞鑫,阮春燕，等.‘圣剑’洋桔梗植株高频再生体系建立及卡那霉素敏感性测定[J].广西植物,2014,34(1)：120-125. [24]袁学军，陈光宙，李艳丽．甜叶菊种子丛生芽诱导和植株再生[J].草原与草坪,2010,33(6):55-56.
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备注/Memo
第一作者简介：刘步仓（1987-），男，甘肃镇原人，硕士研究生，研究方向为植物适应逆境的分子机理。E-mail：liubc1988@163.com. 责任作者：祝建波（1967-），男，博士，研究员，现主要从事植物基因工程及植物逆境生理等研究工作。E-mail：zjbshz@126.com. 基金项目：兵团创新团队建设资助项目(2014C005)。