TANG Yu-qin,LIU Ke-zhen,ZHANG Yu,et al.Study on Effect of Fomitopsis ulmaria (Sor.:Fr.)Bond.et Sing Phellinus Polysaccharide on Removal of DPPH Freedom Radical and Bacterostation Action[J].Northern Horticulture,2015,39(06):133-135.[doi:10.11937/bfyy.201506037]
榆生拟层孔菌粗多糖对DPPH自由基清除及抑菌作用研究
- Title:
- Study on Effect of Fomitopsis ulmaria (Sor.:Fr.)Bond.et Sing Phellinus Polysaccharide on Removal of DPPH Freedom Radical and Bacterostation Action
- Keywords:
- Fomitopsis ulmaria (Sor.:Fr.)Bond.et Sing; polysaccharides; DPPH freedom radical; antioxidant
- 文献标志码:
- A
- 摘要:
- 以榆生拟层孔菌粗多糖为试材，研究榆生拟层孔菌粗多糖对DPPH自由基清除及抑菌作用。结果表明：榆生拟层孔菌粗多糖对DPPH自由基具有清除作用，清除率与多糖浓度在一定范围内呈线性关系，即随着粗多糖浓度的增加，清除率逐渐上升。榆生拟层孔菌粗多糖对霉菌几乎没有抑菌作用，而对细菌有较好的抑制作用，对酵母菌也有一定的抑菌作用。说明榆生拟层孔菌粗多糖具有一定的抗氧化及抑菌作用。
- Abstract:
- Taking Fomitopsis ulmaria (Sor.:Fr.)Bond et Sing polysaccharides as the research object，the DPPH radical scavenging ability of the test method conducted elm Phellinus polysaccharide inhibitory effect and anti-oxidation were studied.The results showed that the inhibitory effect of the selected fungal inhibition zone does not appear on Escherichia coli and yeast minimal inhibitory concentration 2.250 mg/mL，the smallest of the four bacteria and Staphylococcus aureus associated suppression concentration of bacteria circle 4.500 mg/mL，the minimum inhibitory concentration of Bacillus subtilis was 9.000 mg/mL，research in anti-oxidation polysaccharide concentration in the range of 0.075—0.150 mg/mL DPPH radical scavenging capacity was not obvious，in with increasing concentration of polysaccharides significantly increased within the range of 0.150—0.600 mg/mL.
参考文献/References:
[1]张俊会,王谦.杏鲍菇多糖的抗氧化活性研究[J].中国食用菌,2003,22(2):38-39. [2]韩天龙,李知平.食用菌子实体多糖的提取工艺研究和保健作用探讨[J].吉林工程技术师范学院学报,2005(3):14-16. [3]朱美静.猴头菌多糖的提取及理化性质研究[D].无锡:江南大学,2006. [4]刘莹.三种食用菌粗多糖体外抗菌活性研究[J].食用菌,2009(2):66-68. [5]董爱文,陈建华,周辉,等.爬山虎多糖的提取及抑菌作用[J].广州食品工业科技,2003，19(3):15-17. [6]辛英姬,方绍海,王筱凡,等.茶树菇多糖抑菌效果的实验[J].食用菌,2011(4):64-65. [7]彭长连,陈少薇.用清除自由基DPPH法评价植物的抗氧化能力[J].生物化学与生物物理进展,2000,27(6):658-661. [8]盛伟,方晓阳.杏鲍菇菌丝体胞内与胞外多糖体外抗氧化活性研究[J].中国林副特产,2009(1):7-10. [9]顾有芳,李卫民,李升和,等.大枣多糖对小鼠四氯化碳诱发肝损伤防护作用的实验研究[J].中国中医药科技,2006,13(2):105-107. [10]郑晶泉.抗氧化剂抗氧化实验研究进展[J].国外医学,2000,27(1):37-40.
备注/Memo
第一作者简介：唐玉琴（1964-），女，硕士，教授，现主要从事微生物及食用菌等研究工作。E-mail：335673084@qq.com. 基金项目：微生物重点学科培育资助项目(吉农院合字[2013]第X003号)；吉林省高等学校大学生创新训练资助项目；吉林农业科技学院大学生科技创新资助项目。