[1]赵晓帼,朱毅.壳聚糖处理对萝卜芽苗菜品质及其生理生化特性的影响[J].北方园艺,2014,38(22):9-13.
　ZHAO Xiao-guo,ZHU Yi.Effect of Chitosan Treatment on Sprouts Physiological and Biochemical Property of Radish[J].Northern Horticulture,2014,38(22):9-13.
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壳聚糖处理对萝卜芽苗菜品质及其生理生化特性的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第38卷 期数: 2014年22期 页码: 9-13 栏目: 出版日期: 2014-11-30
Title:
Effect of Chitosan Treatment on Sprouts Physiological and Biochemical Property of Radish
文章编号:
1001-0009(2014)22-0009-05
作者:
赵晓帼; 朱毅
中国农业大学 食品科学与营养工程学院，北京 100083
Author(s):
ZHAO Xiao-guo; ZHU Yi
College of Food Science and Nutritional Engineering，China Agricultural University，Beijing 100083
关键词:
萝卜; 幼苗; 壳聚糖; 生理生化特性
Keywords:
radish; sprouts; chitosan; physiological and biochemical property
分类号:
S 631.104+.1
文献标志码:
A
摘要:
以“盛丰”品种白萝卜为试材，采用不同浓度（0.1%、0.3%、0.5%）壳聚糖溶液对萝卜种子进行处理，研究了种子萌发后不同生长天数（3、5、7 d）幼苗的生理生化指标（叶绿素、类胡萝卜素、总酚、芥子油苷、DPPH抑制率、PAL活性、CAT活性、POD活性）的变化情况，以探求壳聚糖处理萝卜种子后对其幼苗生理生化特性的影响。结果表明：与对照组（未进行壳聚糖包衣处理）相比，采用壳聚糖处理对萝卜幼苗的生理生化指标均有显著影响。对于生长3 d和5 d的幼苗，3种浓度的壳聚糖处理均能显著提高其叶绿素、类胡萝卜素、总酚、芥子油苷含量；而对于生长7 d的幼苗，只有0.3%和0.5%壳聚糖处理能显著提高其叶绿素、类胡萝卜素、总酚、芥子油苷含量；3种浓度壳聚糖处理均能显著提高生长3、5、7 d的幼苗CAT活性及生长5 d的幼苗PAL活性；0.1%壳聚糖处理能提高生长3 d的幼苗POD活性，0.5%壳聚糖处理能提高生长5、7 d的幼苗POD活性。表明采用壳聚糖处理萝卜种子能提高其幼苗营养物质含量、改善品质，对农业生产具有一定的指导意义。
Abstract:
Adopting “Shengfeng” white radish as experimental material，seeds were coated with different concentrations (0.1%，0.3%，0.5%) of chitosan.The variation of physiological indexes of 3-，5- and 7-day-old sprouts (chlorophyll，carotenoid，total phenolic，total glucosinolate，DPPH inhibition ratio，PAL activity，CAT activity and POD activity) were investigated，to investigate the effect of chitosan treatment on sprouts physiological property of radish.The results showed that the effect of chitosan treatment on sprouts had a significant difference in comparison to the control sprouts.For the 3-and 5-day-old sprouts，the contents of chlorophyll，carotenoid，total phenolic and total glucosinolate were significantly enhanced under 0.1%，0.3% and 0.5% of chitosan treatment.For the 7-day-old sprouts，the contents of chlorophyll，carotenoid，total phenolic and total glucosinolate were significantly increased under 0.3% and 0.5% of chitosan treatment.Besides，the CAT activity of 3-，5- and 7-day-old sprouts and the PAL activity of 5-day-old sprouts were significantly increased under 0.1%，0.3%and 0.5%of chitosan treatment.The POD activity of 3-day-old as well as 5-，7-day-old sprouts was significantly enhanced under 0.1%and 0.5%of chitosan treatment，respectively.It showed the nutritional value of radish sprouts grown from the seeds coated with chitosan could be improved，which could provide guidance for agricultural production．

参考文献/References:

[1]Temple N J.Antioxidants and disease：More questions than answers[J].Nutrition Research，2000（20）:449-459．

[2]Hashimoto K，Kawamata S，Usui N，et al.In vitro induction of the anticarcinogenic marker enzyme，quinone reductase，in human hepatoma cells by food extracts[J].Cancer Letters,2002,180:1-5
[3]Gundgaard J，Nielsen J N，Olsen J，et al.Increased intake of fruit and vegetables：estimation of impact in terms of life expectancy and healthcare costs[J].Public Health Nutrition,2003(6):25-30
[4]Gosslau A，Chen K Y.Nutraceuticals，apoptosis，and disease prevention[J].Nutrition,2004（20）:95-102．[5]Kopsell D E，Kopsell D A，Randle W A，et al.Kale carotenoids remain stable while flavor compounds respond to changes in sulfur fertility[J].Journal of Agricultural and Food Chemistry,2003,51:5319-5325．
[6]Singh J，Upadhyay A K，Prasad K，et al.Variability of carotenes，vitamin C，E and phenolics in Brassica vegetables[J].Journal of Food Composition and Analysis,2007（20）:106-112．
[7]Baenas N，Moreno D A，Garcia-viguera C.Selecting sprouts of brassicaceae for optimum phytochemical composition[J].Journal of Agricultural and Food Chemistry,2012,60:11409-11420
[8]Schonhof I，Blankenburg D，Muller S，et al.Sulfur and nitrogen supply influence growth，product appearance，and glucosinolate concentration of broccoli[J].Journal of Plant Nutrition and Soil Science-Zeitschrift Fur Pflanzenernahrung Und Bodenkunde,2007,170:65-72．
[9]Perez-balibrea S，Moreno D A，Garcia-viguera C.Glucosinolates in Broccoli Sprouts (Brassica oleracea var.italica) as Conditioned by Sulphate Supply during Germination[J].Journal of Food Science,2010,75:C673-C677．
[10]Yuan G F，Wang X P，Guo R F，et al.Effect of salt stress on phenolic compounds，glucosinolates，myrosinase and antioxidant activity in radish sprouts[J].Food Chemistry,2010,121:1014-1019．
[11]Guo R F，Yuan G F，Wang Q M.Sucrose enhances the accumulation of anthocyanins and glucosinolates in broccoli sprouts[J].Food Chemistry,2011,129:1080-1087．
[12]Perez-balibrea S，Moreno D A，Garcia-viguera C.〖JP3〗Improving the phytochemical composition of broccoli sprouts by elicitation[J].Food Chemistry,2011,129:35-44
[13]Zhou C G，Zhu Y，Luo Y B.Effects of sulfur fertilization on the accumulation of health-promoting phytochemicals in radish sprouts[J].Journal of Agricultural and Food Chemistry,2013,61:7552-755
[14]Choi H S，Kim K T，Lee J Y，et al.Effect of presoaking treatments on growth and rot of soybean sprouts[J].Journal of Food Science and Technology,2000（32）:584-589．
[15]No H K，Lee K S，Kim I D，et al.Chitosan treatment affects yield，ascorbic acid content，and hardness of soybean sprouts[J].Journal of Food Science,2003,68:680-685．
[16]Cho M H，Prinyawiwatkul W.Chitosan treatments affect growth and selected quality of sunflower sprouts[J].Journal of Food Science,2008,73:70-77．
[17]王学奎.植物生理生化试验原理和技术 [M].2版.北京:高等教育出版社,2006．
[18]Ainsworth E A，Gillespie K M.Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin-Ciocalteu reagent[J].Nature Protocols,2007(2):875-877
[19]李培武,周海燕.油菜硫代葡萄糖苷检测技术研究进展[J].中国油料作物学报,2008,30(1)：127,131-136
[20]刘念,蒙大庆,汤天泽,等.油菜硫代葡萄糖苷检测技术研究进展[J].湖北农业科学，2011(7):1301-130
[21]赵玉梅,曹姜.果蔬采后生理生化试验指导[M].北京:中国轻工业出版社:2011．
[22]张爱琴,朱钟.植物生理学试验[M].北京:北京大学出版社,1990．
[23]张素霞.菠菜叶中叶绿素提取工艺研究[J].中国食物与营养,2008(5):40-43．
[24]胡景江,左仲武,刘彦超.壳聚糖对油松种子萌发及幼苗生理生化特性的影响[J].西北林学院学报,2003(4):21-23．
[25]郭东会,朱启忠,胡述浩.干旱胁迫下壳聚糖对花生幼苗生理特性的影响[J].资源开发与市场,2012(8):676-678．
[26]李福枝,刘飞,曾晓希,等.天然类胡萝卜素的研究进展[J].食品工业科技,2007(9):227-232．
[27]Kopsell D A，Barickman T C，Sams C E，et al.Influence of nitrogen and sulfur on biomass production and carotenoid and glucosinolate concentrations in watercress (Nasturtium officinale R.Br.)[J].Journal of Agricultural and Food Chemistry,2007,55:10628-10634．
[28]Reif C，Arrigoni E，Scharer H，et al.Carotenoid database of commonly eaten Swiss vegetables and their estimated contribution to carotenoid intake[J].Journal of Food Composition and Analysis，2013（29）:64-72．
[29]Kim H J，Chen F，Wang X，et al.Effect of methyl jasmonate on phenolics，isothiocyanate，and metabolic enzymes in radish sprout (Raphanus sativus L.)[J].Journal of Agricultural and Food Chemistry,2006,54:7263-7269．
[30]Fahey J W，Wehage S L，Holtzclaw W D，et al.Protection of humans by plant glucosinolates：efficiency of conversion of glucosinolates to isothiocyanates by the gastrointestinal microflora[J].Cancer Prevention Research,2012(5):603-611．
[31]Guerrero-beltran C E，Calderon-oliver M，Pedraza-chaverr I J，et al.Protective effect of sulforaphane against oxidative stress：Recent advances[J].Experimental and Toxicologic Pathology,2012,64:503-508．
[32]Yuan Y X，Chiu L W，Li L.Transcriptional regulation of anthocyanin biosynthesis in red cabbage[J].Planta,2009,230:1141-1153．
[33]裴倩如.PVDC包膜处理对黄瓜低温贮藏冷害的影响[J].安徽农业科学,2013,41(18):7936,7938-7974
[34]Blokhina O V E，Fagerstedt K V.Antioxidants，oxidative damage and oxyen deprivation stress：a review[J].Annals of Botany,2003,91:179-194．
[35]Sala J M，Lafuente M T.Catalase enzyme activity is related to tolerance of mandarin fruits to chilling[J].Postharvest Biology and Technology,2000（20）:81-89．

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备注/Memo

第一作者简介：赵晓帼（1989-），女，硕士研究生，研究方向为食品科学。E-mail：zhaoxg_runner@163.com．

责任作者：朱毅（1973-），女，博士，副教授，现主要从事果蔬采后保鲜与生物活性物质功效等研究工作。E-mail:zhuyi@cau.edu.cn．
基金项目：国家自然科学基金资助项目（31101263）。
收稿日期：2014-07-14

更新日期/Last Update: 2014-12-10