LIU Xu,LIANG Man,WANG Yang,et al.Study on the Pathogen Biological Characteristics and Pathogenicity of Grape Downey Mildew from Different Areas[J].Northern Horticulture,2013,37(06):119-122.
不同地区葡萄霜霉病菌生物学特性及致病力研究
- Title:
- Study on the Pathogen Biological Characteristics and Pathogenicity of Grape Downey Mildew from Different Areas
- 文章编号:
- 1001-0009(2013)06-0119-04
- Keywords:
- downey mildew; biological characteristics; pathogenicity; grape
- 分类号:
- S 436.631.1
- 文献标志码:
- A
- 摘要:
- 以“红地球”葡萄为试材，研究了重庆和陕西地区葡萄霜霉病菌的生物学特性，比较分析了其致病力差异。结果表明：重庆和陕西地区葡萄霜霉病的孢子囊形态差异较小，但陕西地区的孢子囊梗更长，分支更多。硝酸铵、蛋白胨和氯化铵作为氮源，以及葡萄糖、麦芽糖和蔗糖作为碳源均对孢子囊的萌发具有一定的抑制作用。该病菌在pH 4.5～8.5的培养条件均能萌发，但重庆地区孢子囊萌发的最适pH值高于陕西。2个地区霜霉病孢子囊最佳的萌发温度为20℃，且在水膜中的萌发率最高。叶盘法离体培养下，2个地区葡萄霜霉病菌的病级指数集中在0和1，且以1为主。
- Abstract:
- Taking ‘Hongdiqiu’ grape as material，the pathogen biological characteristics and pathogenicity of grape downey mildew from Shaanxi and Chongqing were studied.The results showed that there was no obvious difference on morphological property for sporangium from these two areas.However，the longer sporangiophore and more branches were observed for the downey mildew from Shaanxi areas.Furthermore，all the nitrogen sources including ammonium nitrate，peptone and ammonium chloride showed inhibitory effect on germination sporangium from both the two areas.The three carbon source including glucose，maltose syrup and sucrose had same effect on sporangium germination.The sporangium could germinated at the pH value between 4.5～8.5.However，the optimum pH value for germination sporangium from Shaanxi was higher than that of Chongqing.The optimum temperature was 20℃ for germination sporangium from the two areas，which showed highest germination rate under conditions of water film.The disease index in leaf disks was mainly 0 and 1 by the culture in vitro，and grade 1 was mostly observed.
参考文献/References:
[1]
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备注/Memo
第一作者简介：刘旭（1980-），男，博士，讲师，现主要从事葡萄与葡萄酒相关研究工作。E-mail:liuxu@nwsuaf.edu.cn.