XIE Li,ZHOU Huan,ZHANG Zhi-sheng,et al.Research on Micropropagation Technology of Aspidistra elatior[J].Northern Horticulture,2013,37(24):80-83.
一叶兰的快速繁殖技术研究
- Title:
- Research on Micropropagation Technology of Aspidistra elatior
- 文章编号:
- 1001-0009（2013）24-0080-04
- Keywords:
- Aspidistra elatior; rapid propagation; callus; induction; differentiation; root differentiation
- 分类号:
- Q 81
- 文献标志码:
- A
- 摘要:
- 以一叶兰无菌苗幼叶为外植体，研究了一叶兰愈伤组织诱导、分化和生根的影响因素。结果表明：基本培养基、放置方式、外植体大小、植物生长调节剂和活性炭对一叶兰愈伤组织的诱导有显著影响，将叶片切割为0.5 cm2，水平接入1/2 MS+TDZ 3.0 mg/L+NAA 0.10 mg/L+柠檬酸0.10 g/L培养基中45 d，愈伤组织诱导率最高，为31.25%；培养方式、切割处理、TDZ和继代次数对一叶兰的芽分化有显著影响，将愈伤组织转接到1/2 MS+TDZ 3.00 mg/L+NAA 0.10 mg/L+柠檬酸0.10 g/L培养基中，分化率为114.81%；NAA浓度和活性炭对生根影响显著，在1/2 MS+6-BA 0.10 mg/L+NAA 1.00 mg/L+活性炭1.00 g/L培养基中培养30 d，生根率为39.26%。
- Abstract:
- Using young leaves of Aspidistra elatior as explant,the induction，bud differentiation and root differentiation of the callus of Aspidistra elatior were studied.The results showed that the basic medium，inoculation manner，size of explant，plant growth regulator，citric acid and active carbon significantly influenced the induction of callus.The induction rate was highest(31.25%) when leaf segments of 0.5 cm2were horizontally inoculated on the medium of 1/2 MS+TDZ 3.00 mg/L+NAA 0.10 mg/L+citric acid 0.10 g/L for 45 days.Culture method，cutting treatment，TDZ and the number of subculture significantly affected the differentiation rate of bud.The bud differentiation rate was the highest(114.81%) when callus cultured in 1/2 MS+TDZ 3.00 mg/L+NAA 0.10 mg/L+citric acid 0.1 g/L.NAA and active carbon significantly influenced root differentiation.When the buds cultured in 1/2 MS+6-BA 0.10 mg/L+NAA 1.00 mg/L+active carbon 1.00 mg/L for 30 days，the root differentiation rate was 39.26%.
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备注/Memo
第一作者简介：谢利（1974-），女，博士，讲师，研究方向为花卉遗传育种及组织培养。E-mail：xieli@scau.edu.cn.
责任作者：郭和蓉（1964-），女，博士，副教授，现主要从事花卉栽培等工作。E-mail:guoherong@scau.edu.cn.
基金项目：农业部‘948’资助项目（2008-Z19）。
收稿日期：2013-09-23