LI Shu-ping,LIU Mei-ying,SUN Qing-tian,et al.Study on Isolation and Identification Fungi Pathogen Causing Root Crown Rot in Cherry[J].Northern Horticulture,2013,37(15):130-132.
大樱桃根茎腐烂致病菌的分离鉴定
- Title:
- Study on Isolation and Identification Fungi Pathogen Causing Root Crown Rot in Cherry
- 文章编号:
- 1001-0009(2013)15-0130-03
- Keywords:
- cherry; root crown rot; Ceriporia lacerate
- 分类号:
- S 662.5
- 文献标志码:
- A
- 摘要:
- 以感根茎腐烂病的樱桃树为试材，对其病原菌进行分离纯化、致病性检测和r-DNA-ITS序列分析，研究了pH和温度对病原菌菌丝生长的影响。结果表明：分离到的4株病原菌中仅1株引起了根茎腐烂病，经rDNAITS序列分析鉴定，该菌为撕裂蜡孔菌；该菌在pH 4.0~7.0生长较快，最适宜pH为6.0；最适生长温度为32~34℃，最高生长温度38℃，致死温度42℃。
- Abstract:
- Taking cherry collar rot tree as material,the pathogen was isolated and purified,and its pathogenicity was tested and r-DNA-ITS sequence was analyzed.The effect of pH and temperature on growth of mycelium were also studied.The results showed that four strains were isolated but only one strain could cause cherry collar rot.The pathogen was Ceriporia lacerate based on r-DNA-ITS sequence analysis.The ideal pH for mycelium was 4.0~7.0,the optimum pH was 6.0.The optimum growth temperature for the mycelium was 32~34℃,the maximum growth temperature was 38℃ and the lethal temperature was 42℃.
参考文献/References:
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[2]Jae Won Lee,ki Seob Gwak,Jun Yeong Park.Biological pretreatment of softwood pinus densiflora by three white rot fungi[J].The Journal of Microbiology,2007(10):485-491.
[3]岳爱玲,张悦,姚琼,等.一株撕裂蜡孔菌的分离培养及其系统发育分析[J].生物技术,2010,20(5):47-50.
[4]崔宝凯.紫金山新发现一种多孔菌[DB/OL].http://news.sina.com.cn/c/edu/2006-05-19/07298965711s.shtm/.
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备注/Memo
第一作者简介：李淑平（1975-），女，山东德州人，硕士，高级农艺师，现主要从事樱桃病害防治等研究工作。E-mail:lsp2008@163.com.
责任作者：张福兴（1962-），男，山东烟台人，本科，研究员，现主要从事樱桃品种选育及栽培技术等研究工作。E-mail:gsszfx@163.com.
基金项目：国家公益性行业（农业）科研专项资助项目（200903019）；烟台市科学技术攻关计划资助项目（2008107）。
收稿日期：2013-03-11