[1]姜生秀,兰海燕.杏仁基因组DNA微量提取的改良方法[J].北方园艺,2012,36(20):105-107.
　JIANG Sheng-xiu,LAN Hai-yan.The Improved Method of Apricot Genomic DNA Trace Extraction[J].Northern Horticulture,2012,36(20):105-107.
点击复制

杏仁基因组DNA微量提取的改良方法

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第36卷 期数: 2012年20期 页码: 105-107 栏目: 出版日期: 2012-10-30
Title:
The Improved Method of Apricot Genomic DNA Trace Extraction
文章编号:
1001-0009（2012）20-0105-03
作者:
姜生秀; 兰海燕
新疆大学 生命科学与技术学院，新疆生物资源与基因工程重点实验室，新疆 乌鲁木齐 830046
Author(s):
JIANG Sheng-xiu; LAN Hai-yan
College of Life Science and Technology，Xinjiang University，Xinjiang Key Laboratory of Biological Resources and Gene Engineering，Urumqi,Xinjiang 830046
关键词:
杏仁; 基因组; SDS
Keywords:
almond; genome; SDS
分类号:
Q 946.2
文献标志码:
B
摘要:
基于报道的大豆干种子提取方法，对高脂肪的杏仁进行了基因组DNA提取方法的改良研究，并以提取的基因组为模板进行了PCR扩增验证。结果表明：改良的SDS法提取的杏仁基因组DNA具有纯度和浓度高、条带清晰、降解少等优点，并在高倍稀释条件下仍能获得有效扩增。
Abstract:
Based on the genomic extraction method reported in soybean seeds，the improved method was used for genomic DNA isolation of high fat almond，and the genome extracted as a template for the PCR amplification verification.The results showed that the DNA extractioned in improved SDS method had these advantages：purity and concentration was high，the strip was clear，degradation was less，and in high dilution conditions could still obtain effective amplification.

参考文献/References:

[1]王振东,孙仓,王惠.不同方法从大豆不同组织中提取基因组DNA效果的比较[J].大豆科学,2008,27(1):42-45.

[2]朱振雷,束永俊,李勇,等.大豆种子DNA快速提取方法的改良及应用[J].东北大学农业学报,2009,40(10):60-63.

[3]田苗苗,周延清,牛敬媛,等.单粒干燥大豆种子基因组DNA提取的有效方法[J].生物学通报,2005,40(10)：38-39.

[4]刘艳玲,徐立铭,程中平.基于ITS序列探讨核果类果树桃、李、杏、梅、樱的系统发育关系[J].园艺学报,2007,34(1):23-28.

[5]熊劲芳,向育君,张正奇.花生总DNA的快速提取与纯化研究[J].生物学杂志,2003,20(2):32-34.

[6]魏琦超,畅丽萍,周岩,等.一种简便实用的玉米干种子基因组DNA提取方法[J].广东农业科学,2009（6）:165-167.

[7]王晓丹,吕慧颖,张敬,等.以PCR为目的的大豆叶片DNA提取方法的比较研究[J].分子植物育种,2004,2(6):891-894.


相似文献/References:

[1]张宏磊,朱杰华,胡珍珠,等.致病疫霉基因组序列微卫星的发掘与开发分析[J].北方园艺,2013,37(17):110.
　ZHANG Hong-lei,ZHU Jie-hua,HU Zhen-zhu,et al.Detection and Analysis of Microsatellites in the Phytophthora infestans Genome[J].Northern Horticulture,2013,37(20):110.
[2]王峰,丁云花,李成琼,等.植物异源附加系研究进展[J].北方园艺,2012,36(19):195.
　WANG Feng,DING Yun-hua,LI Cheng-qiong,et al.Research Progress in Alien Plant Additional Lines[J].Northern Horticulture,2012,36(20):195.
[3]高兴旺,王贤磊,杨雅婷,等.甜瓜基因组SSR位点分布规律与多态性初探[J].北方园艺,2014,38(15):93.
　GAO Xing-wang,WANG Xian-lei,YANG Ya-ting,et al.Distribution and Polymorphism Characteristics of SSR Loci in Melon Genome[J].Northern Horticulture,2014,38(20):93.
[4]王晓娥,姚方杰.真菌降解木质纤维素酶系的研究进展[J].北方园艺,2015,39(03):176.[doi:10.11937/bfyy.201503051]
　WANG Xiao-e,YAO Fang-jie.Research Progress on Lignocellulose Degradation Enzyme System of Fungi[J].Northern Horticulture,2015,39(20):176.[doi:10.11937/bfyy.201503051]
[5]吴新义,徐沛,吴晓花,等.瓠瓜基因组测定[J].北方园艺,2016,40(02):110.[doi:10.11937/bfyy.201602029]
　WU Xinyi,XU Pei,WU Xiaohua,et al.Estimation of Genome of Bottle Gourd[J].Northern Horticulture,2016,40(20):110.[doi:10.11937/bfyy.201602029]
[6]许瑞瑞,高明刚,刘春香.苹果基因组中转录因子的鉴定与分析[J].北方园艺,2018,42(09):42.[doi:10.11937/bfyy.20173398]
　XU Ruirui,GAO Minggang,LIU Chunxiang.Identification and Analysis of Transcription Factors in Apple Genome[J].Northern Horticulture,2018,42(20):42.[doi:10.11937/bfyy.20173398]
[7]高耀辉,马斌,肖凤洁,等.CRISPR/Cas9系统在园林植物中的研究展望[J].北方园艺,2019,43(15):133.[doi:10.11937/bfyy.20183493]
　GAO Yaohui,MA Bin,XIAO Fengjie,et al.Prospect of Research on CRISPR/Cas9 System in Garden Plants[J].Northern Horticulture,2019,43(20):133.[doi:10.11937/bfyy.20183493]
[8]黄雪,韩爱芝,图尔荪古丽?吾拉伊木,等.南疆果仁兼用杏种仁矿物质元素鉴评[J].北方园艺,2021,(03):19.[doi:10.11937/bfyy.20202155]
　HUANG Xue,HAN Aizhi,TuersunguliWULAYIMU,et al.Identification of Mineral Elements of Apricot Seed Kernels Used in Combination With Fruit Kernels in Southern Xinjiang[J].Northern Horticulture,2021,(20):19.[doi:10.11937/bfyy.20202155]
[9]李雪松,华蓉,孙达锋,等.基于花脸香蘑基因组的SSR分子标记开发[J].北方园艺,2023,(02):112.[doi:10.11937/bfyy.20222019]
　LI Xuesong,HUA Rong,SUN Dafeng,et al.Development of SSR Molecular Markers Based on Whole Genome of Lepista sordida[J].Northern Horticulture,2023,(20):112.[doi:10.11937/bfyy.20222019]
[10]焦枥禾,王凤利,马银鹏,等.黑木耳分子生物学研究进展[J].北方园艺,2023,(17):124.[doi:10.11937/bfyy.20230365]
　JIAO Lihe,WANG Fengli,MA Yinpeng,et al.Research Progress on Molecular Biology of Auricularia heimuer[J].Northern Horticulture,2023,(20):124.[doi:10.11937/bfyy.20230365]

备注/Memo

第一作者简介：姜生秀（1987-），女，在读硕士，研究方向为生物化学与分子生物学。E-mail：yanyunjiang1987@163.com.

更新日期/Last Update: 2014-08-31