ZHAO Yu-fen,CHU Bo-yan,YIN Xin-yan,et al.Study on the Optimization for the Tube Seedlings Rooting of Hemerocallis hybridas by Using the Orthogonal Experiment[J].Northern Horticulture,2013,37(06):100-102.
应用正交实验优化大花萱草试管苗生根的研究
- Title:
- Study on the Optimization for the Tube Seedlings Rooting of Hemerocallis hybridas by Using the Orthogonal Experiment
- 文章编号:
- 1001-0009(2013)06-0100-03
- 分类号:
- S 682.1+9
- 文献标志码:
- A
- 摘要:
- 采用L9(33)正交实验设计,研究了IBA(0.1、0.3、0.5 mg/L)、白砂糖(25、35、45 g/L)、琼脂(3、4、6 g/L)3因素3水平组合对大花萱草“红运”试管丛生芽苗生根的影响。结果表明:3个因素中糖分对其生根有显著影响,其大小排序为白砂糖>IBA>琼脂。筛选出1/2MS+IBA 0.3 mg/L+白砂糖45 g/L+琼脂4 g/L为最佳组合
- Abstract:
- By using L9(33) orthogonal experimental design,Co-action of IBA and white granulated sugar and agar on the caespitose shoots rooting of Hemerocallis hybridas were studied.In the experiment,the concentration of IBA was 0.1,0.3,0.5 mg/L;the concentration of white granulated sugar were 25,35,45 g/L;the concentration of agar were 3,4,6 g/L.The results showed that the effects of each factor on the tube seedlings rooting was white granulated sugar>IBA>agar,the action of sugar reached at significant level,and 1/2MS+IBA 0.3 mg/L+white granulated sugar 45 g/L+agar 4 g/L was the optimal combination.
参考文献/References:
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备注/Memo
第一作者简介:赵玉芬(1974-),女,本科,高级工程师,现主要从事园林植物的栽培及组织培养技术等研究工作。E-mail:hbzyf74@163.com.