YANG Yong,YAN Junfeng,YANG Weimin,et al.Functional Study on the Involvement of the BoNAC02 Gene in Abscisic Acid and Drought Stress Responses in Kale[J].Northern Horticulture,2025,(23):1-10.[doi:10.11937/bfyy.20251724]
羽衣甘蓝BoNAC02基因参与脱落酸和干旱胁迫响应的功能研究
- Title:
- Functional Study on the Involvement of the BoNAC02 Gene in Abscisic Acid and Drought Stress Responses in Kale
- 文章编号:
- 1001-0009(2025)23-0001-10
- Keywords:
- BoNAC02; kale; drought stress; functional genomics; ABA
- 分类号:
- S 635
- 文献标志码:
- A
- 摘要:
- 以羽衣甘蓝为试材，采用基因过表达与沉默技术结合干旱胁迫处理，并运用分子与生理学分析方法，研究了BoNAC02通过ABA信号通路调控植物抗旱性的分子机制，以期揭示该基因在羽衣甘蓝抗旱反应中的功能，为羽衣甘蓝抗旱育种提供了新的基因资源与参考依据。结果表明：BoNAC02表达受干旱与ABA处理显著诱导；其沉默植株抗旱性降低，而过表达植株抗旱性增强，该调控过程依赖于ABA信号途径；同时，BoNAC02通过正向调节ABA信号通路及相关抗氧化酶代谢基因的表达，增强植物对干旱的响应能力。
- Abstract:
- Taking kale(Brassica oleracea var.acephala) as the test material,gene overexpression and silencing techniques were combined with drought stress treatment,and molecular and physiological analysis methods were used to study the molecular mechanism of BoNAC02 regulating plant drought resistance through the ABA signaling pathway,in order to reveal the function of this gene in the drought response of kale and provide both new genetic resources and reference for improving drought tolerance in kale through breeding.The results showed that BoNAC02 expression was significantly induced by both drought and ABA treatments.Silencing of BoNAC02 markedly reduced drought tolerance，whereas its overexpression enhanced it，a regulatory process dependent on the ABA signaling pathway.Furthermore，BoNAC02 enhances the plant′s drought response capacity by positively regulating the ABA signaling pathway and the expression of genes related to antioxidant enzyme metabolism.
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备注/Memo
第一作者简介：杨勇(1982-),男，硕士，工程师，现主要从事园林植物等研究工作。E-mail:461556594@qq.com.责任作者：李大红（1969-）,男，博士，教授，现主要从事植物学等研究工作。E-mail:lidahong@huanghuai.edu.cn.基金项目：河南省科技厅科技攻关资助项目（252102110204）。收稿日期：2025-05-10