WU Jinli,DU Minru,LIAN Lingdan,et al.Identification and Preliminary Functional Study of PAL Gene Family in Pleurotus eryngii[J].Northern Horticulture,2025,(18):37-44.[doi:10.11937/bfyy.20250252]
杏鲍菇PAL基因家族的鉴定及其功能初探
- Title:
- Identification and Preliminary Functional Study of PAL Gene Family in Pleurotus eryngii
- 文章编号:
- 1001-0009(2025)18-0037-08
- 分类号:
- S 646
- 文献标志码:
- A
- 摘要:
- 以杏鲍菇为试材，采用生物信息学和qRT-PCR方法，研究了杏鲍菇PAL基因家族成员的理化性质、系统进化关系和基因启动子顺式作用元件，以及PePALs基因在杏鲍菇受到不同非生物胁迫、不同生长发育阶段和不同组织下的表达特征，以期为PAL基因在杏鲍菇抗逆方面的研究提供参考依据。结果表明：杏鲍菇PAL基因家族共有2个成员，为PePAL1和PePAL2，分别编码743个和747个氨基酸，等电点为5.28和6.39。PePALs编码蛋白属于疏水性蛋白，亚细胞定位于细胞质，二级结构主要以α-螺旋和无规则卷曲为主。系统发育树显示，PePAL1和PePAL2分别与平菇PAL、白黄侧耳PAL聚为一支。PePAL1和PePAL2基因启动子区域存在较多的逆境应答元件。QRT-PCR结果表明，PePAL1和PePAL2基因在杏鲍菇不同生长发育阶段和不同组织中均有表达，且均在原基时期和菌柄部位表达量最高。PePALs基因对镉、酸、碱、干旱、过氧化氢、盐、冷和热等多种非生物胁迫表现出不同程度的响应，其中PePAL1和PePAL2基因受到热胁迫48 h后，表达量最高达4.41倍和21.72倍。因此，PePAL1和PePAL2基因在杏鲍菇生长发育和响应非生物过程中具有重要作用。
- Abstract:
- Taking Pleurotus eryngii as the test material，bioinformatics and qRT-PCR methods were used to investigate the physicochemical properties，phylogenetic relationships，and promoter cis-acting elements of the PAL gene family members in Pleurotus eryngii.Additionally，the expression characteristics of PePALs genes under different abiotic stresses，at different growth stages，and in different tissues of Pleurotus eryngii were studied，in order to provide reference for exploring the function of PAL genes in stress resistance in Pleurotus eryngii.The results showed that the PAL gene family of Pleurotus eryngii consisted of two members，PePAL1 and PePAL2，encoding 743 and 747 amino acids，with isoelectric points of 5.28 and 6.39，respectively.The PePALs proteins that were hydrophobic，subcellularly localized in the cytoplasm，with secondary structures dominated by α-helices and random coils.Phylogenetic tree showed that PePAL1 and PePAL2 were clustered with Pleurotus ostreatus PAL and Pleurotus cornucopiae PAL，respectively.There were many adversity response elements in the promoter regions of PePAL1 and PePAL2 genes.The results of QRT-PCR showed that PePAL1 and PePAL2 genes were expressed in different growth stages and tissues of Pleurotus eryngii，and both had the highest expression in the primordial stage and stipe.PePALs genes exhibited varying degrees of response to various abiotic stresses such as cadmium，acid，alkali，drought，hydrogen peroxide，salt，cold，and heat.Among them，the PePAL1 and PePAL2 genes had the highest expression levels，with PePAL1 at 4.41 times and PePAL2 at 21.72 times after 48 hours of heat stress.In summary，PePAL1 and PePAL2 genes play important roles in the growth，development，and response to abiotic processes of Pleurotus eryngii.
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备注/Memo
第一作者简介：吴锦丽（1999-），女，硕士研究生，研究方向为食用菌育种与保鲜。E-mail：1832015895@qq.com.责任作者：王杰（1978-），女，博士，教授，现主要从事食药用菌遗传育种、贮藏保鲜与加工等研究工作。E-mail：wjcasey@scau.edu.cn.基金项目：以农产品为单元的广东省现代农业产业技术体系创新团队建设资助项目（农业微生物）(2024CXTD04)；国家自然科学基金青年科学基金资助项目(32302631)。收稿日期：2025-01-23