WANG Shiqi,LI Renping,SONG Lizhi,et al.Effects of Intercropping Belamcanda chinensis on the Structure and Function of Soil Bacterial Community in Poplar Forest Land[J].Northern Horticulture,2024,(19):81-89.[doi:10.11937/bfyy.20240779]
间作射干对杨树林地土壤细菌群落结构和功能的影响
- Title:
- Effects of Intercropping Belamcanda chinensis on the Structure and Function of Soil Bacterial Community in Poplar Forest Land
- 文章编号:
- 1001-0009(2024)19-0081-09
- 分类号:
- S 714.3
- 文献标志码:
- A
- 摘要:
- 以杨树单作土壤、杨树-射干间作系统边缘土壤和中心土壤样品为试材，采用高通量测序方法，研究了间作射干对杨树林地土壤细菌群落组成及功能的影响，以期为筛选杨树林下种植射干的适宜距离提供参考依据。结果表明：间作和单作模式下土壤细菌群落的丰富度指数（Chao1和ACE）、多样性指数（Shannon和Simpson）均无显著差异。土壤变形菌门（Proteobacteria）、酸杆菌门（Acidobacteriota）为不同处理土壤细菌群落的优势菌门，鞘氨醇单胞菌属（Sphingomonas）为优势菌属，间作提高了土壤细菌群落芽单孢菌门（Gemmatimonadota）和绿弯菌门（Chloroflexi）相对丰度，其中B组土壤中的芽单胞菌属（Gemmatimonas）和鞘氨醇单胞菌属丰度升高。根据LEfSe分析发现，B组土壤中有4种细菌类群明显偏高（LDA>4），包括门水平的变形菌门，纲水平的α-变形菌纲（α-Proteobacteria）和Saccharimonadia，科水平的罗丹诺杆菌属(Rhodanobacteraceae)。进一步PICRUSt2功能预测显示，间作提高了碳代谢和丙酮酸代谢功能的相对丰度。综上，杨树-射干间作有助于改善土壤结构，增强土壤代谢功能活性，且种植射干时与杨树留出一定距离为宜。
- Abstract:
- Taking poplar monoculture soil，Poplar-Belamcanda chinens isintercropping system edge soil and centre soil samples as test materials,and high-throughput sequencing method was used，the effects of intercropping with Belamcanda chinensis on the composition and function of soil bacterial community in poplar woodland were studied,in order to provide reference for screening the suitable distance for planting Belamcanda chinensis under poplar forests.The results showed that，there was no significant difference in the richness index (Chao1 and ACE) and diversity index (Shannon and Simpson) of soil bacterial communities between intercropping and monoculture modes.Proteobacteria and Acidobacteriota were the dominant bacterial phyla in soil bacterial communities under different treatments，while Sphingomonas was the dominant bacterial genus.Intercropping increased the relative abundance of soil bacterial communities in the phyla Gemmatimonadota and Chloroflexi，while the abundance of Gemmatimonas and Sphingomonas in group B soil increased.According to LEfSe analysis,there were four bacterial groups in Group B soil that were significantly higher (LDA>4)，included Proteobacteria at the phylum level,AlphaProteobacteria and Saccharimonadia at the class level and Rhodanobacteraceae genus at the family level.Further PICRUSt2 functional prediction showed that intercropping increased the relative abundance of carbon metabolism and pyruvate metabolism functions.In summary，intercropping between poplar and Belamcanda chinensis could help improve soil structure，enhance soil metabolic activity，and it is advisable to leave a certain distance from poplar trees when planting Belamcanda chinensis.
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备注/Memo
第一作者简介：王诗琦（1995-），女，硕士，助理工程师，现主要从事杨树遗传育种等研究工作。E-mail：1033026465@qq.com.责任作者：梁德军（1970-），男，硕士，教授级高级工程师，现主要从事森林培育等研究工作。E-mail：759702894@qq.com.基金项目：国家重点研发计划欧美杨大径级工业资源材精准高效培育技术研究资助项目（2021YFD2201201）。收稿日期：2024-02-26