ZHANG Shudong,LIU Wenfeng,LING Lizhen.Inhibitory Effects of Four Traditional Chinese Medicine Extracts on Postharvest Fungal Phomopsis sp.in ‘Hongyang’ Kiwifruit[J].Northern Horticulture,2023,(24):95-101.[doi:10.11937/bfyy.20232698]
四种中药提取物对‘红阳’猕猴桃软腐病菌拟茎点霉菌的抑制作用
- Title:
- Inhibitory Effects of Four Traditional Chinese Medicine Extracts on Postharvest Fungal Phomopsis sp.in ‘Hongyang’ Kiwifruit
- 文章编号:
- 1001-0009(2023)24-0095-07
- Keywords:
- ‘Hongyang’ kiwifruit; fruit soft; Phomopsis sp.; inhibitory effects; traditional Chinese medicine extracts
- 分类号:
- S 435.6
- 文献标志码:
- A
- 摘要:
- 以‘红阳’猕猴桃为试材，采用菌丝生长速率法，研究了不同浓度八角茴香、花椒、刺梨和两头毛乙醇提取物对拟茎点霉菌的抑制效应，以期探究中药提取物对‘红阳’猕猴桃软腐病的防治作用。结果表明：这4种中药提取物对拟茎点霉菌均表现出了明显的抑制作用，但是在相同浓度下它们的抑制效果有所差异。如在低浓度12.5 mg·mL-1时，八角茴香、花椒、刺梨和两头毛的抑菌率分别为46.82%、53.57%、38.48%和50.80%。而在高浓度100 mg·mL-1时，其抑菌率分别为86.11%、83.73%、66.67%和82.14%。另外，这4种中药提取物对供试菌种菌丝的EC50值分别为15.64、8.76、28.48 mg·mL-1和9.77 mg·mL-1。八角茴香提取物的室内防治效果明显。综上，这4种中药提取物对拟茎点霉菌的抑制作用随着浓度的增加而呈现不同程度的增加，将来可以作为植物杀菌剂资源。
- Abstract:
- The inhibitory effects of the ethanol extracts of Illicium verum，Zanthoxylum bungeanum，Rosa roxburghii and Incarvillea arguta at the different concentrations (0，12.5，25.0，50.0 mg·mL-1and 100.0 mg·mL-1) against the pathogen,one of the postharvest fungi in ‘Hongyang’ kiwifruit，were determined by mycelial growth rate method.The results showed that the ethanol extracts of these Chinese medicine could apparently inhibit the mycelial growth of Phomopsis sp.，but they showed the different inhibitory effects at the same concentration.When the lowest concentration of extracts was 12.5 mg·mL-1，the inhibitory rates of I.verum，Z.bungeanum，R.roxburghii and I.arguta were 46.82%，53.57%，38.49% and 50.80%，respectively.When the concentration was 100.0 mg·mL-1，their inhibitory rates were 86.11%，83.73%，66.67% and 82.14% with EC50 values of 15.64，8.76，28.48 mg·mL-1 and 9.77 mg·mL-1，respectively.These results indicated that all four traditional Chinese medicine extracts showed different increasing of inhibitory effects on Phomopsis sp.with increasing of concentration and can be used as future plant fungicides.
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备注/Memo
第一作者简介：张书东（1979-），男，博士，副教授，现主要从事植物分类与系统进化等研究工作。E-mail：sdchang@foxmail.com.责任作者：凌立贞（1979-），女，博士，副教授，现主要从事果树育种等研究工作。E-mail：121302168@qq.com.基金项目：贵州省科学技术基金资助项目(黔科合基础-ZK［2022］一般530)；大学生创新训练资助项目(S202210977127，S202010977004，S202010977034)。收稿日期：2023-08-07