WANG Xiaochun,YANG Weidi,GAO Ting.Analysis on Bacterial Diversity of Alfalfa Rhizosphere Soil in Saline-alkali Land[J].Northern Horticulture,2023,(23):75-82.[doi:10.11937/bfyy.20231313]
盐碱地紫花苜蓿根际土壤细菌群落多样性分析
- Title:
- Analysis on Bacterial Diversity of Alfalfa Rhizosphere Soil in Saline-alkali Land
- 文章编号:
- 1001-0009(2023)23-0075-08
- Keywords:
- saline-alkali land; alfalfa; rhizosphere soli; bacterial community; high-throughput sequencing
- 分类号:
- S 963.22+3.3
- 文献标志码:
- A
- 摘要:
- 以宁夏银北中重度盐碱地紫花苜蓿根际土壤为研究对象，采集根际及非根际（对照）土壤，开展16S rRNA高通量测序，研究了盐碱地紫花苜蓿根际细菌群落特征，以期为紫花苜蓿种植改良盐碱地及中重度盐碱地微生物资源的研究提供参考依据。结果表明：采样地pH为8.57，盐浓度为669.51 mg·L-1，属于中重度盐碱地。苜蓿根际土壤磷酸酶、蔗糖酶、脲酶活性显著高于非根际，过氧化氢酶活性在二者间无差异。根际与非根际土壤高通量测序共获得了10 488个OTUs，分布于94个门，191个纲，391个目，549个科，910个属，448个种。优势菌门为变形菌（Proteobacteria）、未鉴别菌（unidentified_Bacteria）、拟杆菌（Bacteroidota）、酸杆菌（Acidobacteria）、芽单胞菌（Gemmatimonadetes）；优势菌属为盐单胞菌（Halomonas）、假单胞菌（Pseudomonas）、unidentified_Actinomarinales、固氮弧菌（Azoarcus）。优势菌门变形菌（Proteobacteria）、放线菌（Actinobacteria）、厚壁菌（Firmicutes）相对丰度在根际土壤中极显著高于非根际，优势菌属盐单胞菌（Halomonas）、假单胞菌（Pseudomonas）、固氮弧菌（Azoarcus）相对丰度在根际土壤中显著高于非根际。
- Abstract:
- Take the rhizosphere soil of alfalfa as research object，the rhizosphere and non-rhizosphere (control) soil were collected，and high-throughput sequencing technology of 16S rRNA was used to study the rhizosphere bacterial community characteristics of alfalfa in severe saline-alkali land of Ningxia′ north，in order to provide reference for improving the saline-alkali land by planting alfalfa and using microbial resources of saline-alkali land.The results showed that,soil enzymes activities and microbial diversity in the rhizosphere of alfalfa planted in saline-alkali land.The pH of the sampling area was 8.57，and the salt concentration was 669.51 mg·L-1，which belonged to the middle and severe saline-alkali land.The activities of phosphatase,sucrase and urease in alfalfa rhizosphere soil were significantly higher than those in non-rhizosphere soil.A total of 10 488 OTUs were obtained by high-throughput sequencing,distributed in 94 phyla，191 classes，391 orders,549 families，910 genus and 448 species.The dominant phylum were Proteobacteria,unidentified_Bacteria，Bacteroidota,Acidobacteria and Gemmatimonadetes.The dominant genus were Halomonas，Pseudomonas，unidentified_Actinomarinales and Azoarcus.The relative abundance of dominant bacterial genera Halomonas,Pseudomonas,and Azoarcus was significantly higher in rhizosphere soil than in non rhizosphere soil.
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备注/Memo
第一作者简介：王晓春（1982-），女，硕士，助理研究员，现主要从事牧草种质资源、育种及栽培等研究工作。E-mail：158851531@qq.com.责任作者：高婷（1968-），女，博士，研究员，现主要从事天然草原改良、牧草育种及栽培等研究工作。E-mail：ecogt9@163.com.基金项目：宁夏自然科学基金资助项目（2021AAC03285）；宁夏回族自治区政府农作物育种重大专项资助项目（2014NYZ03）。收稿日期：2023-04-17