LI Qingshan,WANG Mengjiao,LIU Jieran.Diversity and Differences in Soil Microbial Community Structure in the Rhizosphere of Rapeseed at Different Times[J].Northern Horticulture,2023,(24):72-80.[doi:10.11937/bfyy.20231136]
不同时期油菜根际土壤微生物群落结构多样性及其差异
- Title:
- Diversity and Differences in Soil Microbial Community Structure in the Rhizosphere of Rapeseed at Different Times
- 文章编号:
- 1001-0009(2023)24-0072-09
- 分类号:
- S 565.4
- 文献标志码:
- A
- 摘要:
- 以陕西汉中油菜种植基地种植最广的油菜品种“邡油777”的油菜花期和果期的根际土壤样品为试材，采用高通量扩增子的试验方法，对样品根际微生物群落结构进行分析和比较，以期为后续油菜根际促生菌的筛选及油菜-根际土壤-微生物的相互作用关系研究提供参考依据。结果表明：不同时期油菜根际土壤微生物群落结构存在显著性差异，油菜花期土壤样品微生物多样性和丰富度较果期高。群落结构组成中占比大于1%的细菌门10种，占比大于1%的细菌属6种，变形菌门和酸杆菌门是其共有优势菌群；占比大于1%的真菌门7种，占比大于1%的真菌属7种。被孢霉门是油菜根际真菌群落的绝对优势菌群。油菜的不同生长时期是影响油菜根际微生物群落结构和多样性的重要原因。
- Abstract:
- The rhizosphere soil samples of the most widely planted rapeseed variety ‘Fangyou 777’ at the Hanzhong rapeseed planting base in Shaanxi were used as experimental materials.The high-throughput amplicon test method was used to analyze and compare the structure of the rhizosphere microbial community of the samples，in order to provide reference for the subsequent screening of rapeseed rhizosphere growth promoting bacteria and the study of the interaction between rapeseed rhizosphere soil and microorganisms.The results showed that there were significant differences in the microbial community structure of the rhizosphere soil of rapeseed at different stages，and the microbial diversity and richness of the soil samples during the flowering stage of rapeseed were higher than those during the fruit stage.In the composition of the community structure，there were 10 bacterial phyla with a proportion greater than 1%，and 6 bacterial genera with a proportion greater than 1%.Proteobacteria and Acidobacteria were their common dominant bacterial groups.There were 7 species in the phylum of fungi with a proportion greater than 1%，and 7 species in the genus of fungi with a proportion greater than 1%.The Mortierella phylum was the absolute dominant fungal community in the rhizosphere of rapeseed.The different growth stages of rapeseed were important factors affecting the structure and diversity of rhizosphere microbial communities in rapeseed.
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备注/Memo
第一作者简介：李青山（1998-），男，硕士研究生，研究方向为植物根际微生物。E-mail：1279863133@qq.com.责任作者：王梦姣（1987-），女，博士，副教授，现主要从事植物根际土壤微生物等研究工作。E-mail：amy133253@126.com.基金项目：陕西省科技厅青年基金资助项目（2021JQ-755）。收稿日期：2023-04-04