LI Jie,ZHOU Kai,HE Wenfeng.Regulation of Exogenous Ca2+Addition on the Activities of Enzymes Related to Photosynthesis and Nitrogen Metabolism in Climbing Rose Under Salt Stress[J].Northern Horticulture,2023,(19):57-65.[doi:10.11937/bfyy.20231107]
盐胁迫下外源Ca2+添加对藤本月季光合及氮代谢相关酶活性的调控
- Title:
- Regulation of Exogenous Ca2+Addition on the Activities of Enzymes Related to Photosynthesis and Nitrogen Metabolism in Climbing Rose Under Salt Stress
- 文章编号:
- 1001-0009(2023)19-0057-09
- Keywords:
- salt; exogenous Ca2+; climbing rose; photosynthesis; nitrogen metabolism
- 分类号:
- S 685.12
- 文献标志码:
- A
- 摘要:
- 以藤本月季“大游行”为试材，采用土壤喷施0、200 mmol·L-1盐溶液的处理方法，探究不同浓度外源Ca2+（0、5、10、20 mmol·L-1）对藤本月季光合以及氮代谢相关酶活性的影响，以期为外源Ca2+缓解盐胁迫对藤本月季的伤害提供参考依据。结果表明：一定浓度外源Ca2+能够增加藤本月季株高生长量、叶面积和地上生物量，促进光合作用，提高叶片中光合色素含量以及氮代谢相关酶活性来增强植物抗性。在200 mmol·L-1盐处理下，与0 mmol·L-1 Ca2+处理浓度相比，添加10 mmol·L-1 Ca2+浓度处理下藤本月季的叶绿素a、叶绿素b、总叶绿素（a+b）以及叶黄素含量分别增加了143.9%、152.5%、151.9%和90.6%；光合参数中水分利用效率（WUE）、净光合速率（Pn）、蒸腾速率（Tr）、胞间CO2浓度（Ci）和气孔导度（Gs）分别增加96.6%、60.5%、353.3%、43.0%和80.6%，气孔限制值（Ls）降低63.8%，PSⅡ最大光化学效率（Fv/Fm）和PSⅡ潜在活性（Fv/Fo）显著增加；此外，盐胁迫下，藤本月季叶片中硝态氮含量下降，铵态氮含量增加，一定浓度外源Ca2+处理能显著增加硝酸还原酶（NR）、谷氨酰胺合成酶（GS）以及异柠檬酸脱氢酶（NADP-ICDH）活性，而降低谷氨酸脱氢酶（NADH-GDH）活性。综上可知，添加一定量的Ca2+能增加藤本月季光合色素和硝态氮含量，促进氮代谢过程中相关酶活性增加，提高植物光合作用，进一步促进植物生长来抵御盐胁迫，其中以添加10 mmol·L-1Ca2+处理效果最好。
- Abstract:
- Taking climbing rose ‘Parade’ as test material，the effects of different concentrations of exogenous calcium (0,5,10,20 mmol·L-1) on photosynthesis and nitrogen metabolism related enzyme activity in climbing roses were studied by spraying 0，200 mmol·L-1 salt solution onto the soils，in order to provide reference for exogenous Ca2+ to alleviate the damage of salt stress to climbing rose.The results showed that a certain concentration of exogenous Ca2+ could increase the height growth，leaf area，and aboveground biomass of climbing rose，promote photosynthesis，increase the content of photosynthetic pigments in leaves，and enhance the activity of nitrogen metabolism related enzymes to enhance plant resistance.Under 200 mmol·L-1 salt treatment，compared with 0 mmol·L-1 Ca2+ treatment concentration，adding 10 mmol·L-1 Ca2+ concentration increased the chlorophyll a，chlorophyll b，total chlorophyll (a+b)，and lutein content of climbing roses by 143.9%，152.5%，151.9% and 90.6%，respectively.The photosynthetic parameters of water use efficiency (WUE)，net photosynthetic rate (Pn)，transpiration rate (Tr)，intercellular CO2 concentration (Ci) and stomatal conductance (Gs) increased by 96.6%，60.5%，353.3%，43.0% and 80.6%，respectively.The Ls decreased by 63.8%，and the maximum photochemical efficiency (Fv/Fm) and potential activity (Fv/Fo) of PS Ⅱ significantly increased.In addition，under salt stress，the nitrate nitrogen content in the leaves of climbing rose decreased，while the ammonium nitrogen content increased.A certain concentration of exogenous Ca2+ treatment could significantly increase the activities of nitrate reductase (NR)，glutamine synthetase (GS) and isocitrate dehydrogenase (NADP-ICDH)，while reducing the activity of glutamine dehydrogenase (NADH-GDH).The conclusion was that adding a certain amount of Ca2+ could increase the photosynthetic pigments and nitrate nitrogen content of climbing rose，promoted the activity of related enzymes in nitrogen metabolism，improved plant photosynthesis，and further promote plant growth to resist salt stress.Among them，adding 10 mmol·L-1 Ca2+ treatment had the best effect.
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备注/Memo
第一作者简介：李婕（1989-），女，硕士，讲师，现主要从事园林植物与园林生态等研究工作。E-mail：000563@sqxy.edu.cn.基金项目：国家自然科学基金资助项目（32000170）。收稿日期：2023-02-08