YIN Shuli,LIANG Ran,MA Lulu,et al.Analysis on the Structure and Function of Bacterial Community in the Soil Around the Root of Handkea utriformis Based on 16S rDNA High Throughput Sequencing Technology[J].Northern Horticulture,2023,(20):61-68.[doi:10.11937/bfyy.20224757]
基于16S rDNA高通量测序技术的龟裂秃马勃(Handkea utriformis)根围土壤细菌群落结构及功能分析
- Title:
- Analysis on the Structure and Function of Bacterial Community in the Soil Around the Root of Handkea utriformis Based on 16S rDNA High Throughput Sequencing Technology
- 文章编号:
- 1001-0009(2023)20-0061-08
- Keywords:
- Handkea utriformis; high throughput sequencing; bacterial community structure; functional groups
- 分类号:
- Q 939.96
- 文献标志码:
- A
- 摘要:
- 以黑龙江省双鸭山市龟裂秃马勃生长的退化土壤、正常土壤为试材，采用Illumina高通量测序的方法，研究了退化土壤和正常土壤样品中16S rDNA V4~V5区细菌的群落结构及功能差异，探究退化土壤中马勃不再出菇的原因，以期为马勃人工驯化提供参考依据。结果表明：土壤样品测定细菌可操作分类单元归类于2界、44门、128纲、299目、508科、1 048属。退化土壤细菌群落的丰富度指数（Chao1和ACE）、多样性指数（Shannon和InvSimpson）均降低；土壤中拟杆菌门、芽单胞菌门、浮霉菌门及假节杆菌属、RB41、土单胞菌属的丰富度降低，疣微菌门及酸杆菌属、罗丹诺杆菌属的丰富度升高。LEfSe分析结果显示，退化土壤中（LDA>4）的12个优势细菌包括门水平的α-变形菌门、AD3、酸杆菌门，目水平的纤线杆菌目、根瘤菌目、盖勒氏菌目、微胃蛋白目，科水平的微蛋白科、纤线杆菌科、罗丹诺杆菌科；属水平的HSB OF53-F07、Candidatus Solibacter；涉及代谢、有机系统2个一级功能层代谢通路，其它次级代谢产物的生物合成、能量代谢、辅因子和维生素的代谢、萜类化合物和聚酮类化合物的代谢、循环系统、消化系统6个二级代谢通路；较正常土壤中减少了3个一级代谢通路、8个二级代谢通路。退化土壤中细菌菌群结构变化及伴随的代谢功能变化，是导致马勃不能继续生长的重要原因。
- Abstract:
- Taking degraded soil and normal soil from Shuangyashan City,Heilongjiang Province as test materials,the community structure and functional differences of 16S rDNA V4-V5 bacterial communities in degraded soil and normal soil samples were studied using Illumina high-throughput sequencing method,in order to investigate the reasons why Handkea utriformis can not grow in degraded soil and provide reference for artificial domestication of Handkea utriformis.The results showed that the operational taxonomic units belong to 2 kingdoms，44 phyla，128 classes，299 orders，508 families，1 048 genera.Richness index (Chao1 and ACE) and diversity index (Shannon and InvSimpson) of degenerated soil bacterial communities all decreased.In the degenerated soil，the relative abundances of Bacteroidetes，Gemmatimonadetes，Planctomycetes，Pseudarthrobacter，RB41，Terrimonas decreased，and the relative abundances of Verrucomicrobia，Acidothermus，Rhodanobacter increased.LEfSe analysis results showed that in CK soil，12 dominant bacterias were Alphaproteobacteria，AD3，Acidobacteria，Ktedonobacterales，Rhizobiales，Gaiellales，Micropepsales，Micropepsaceae，Ktedonobacteraceae，Rhodanobacteraceae，HSB OF53-F07，Candidatus Solibacter.2 metabolic pathways of the primary functional layers were the metabolism and organic system，6 sub function layers were biosynthesis of other secondary metabolites，energy metabolism，metabolism of cofactors and vitamins，metabolism of terpenoids and polyketones，circulatory system and digestive system.Compared with normal soil，3 primary metabolic pathways and 8 secondary metabolic pathways were reduced.The structural changes of bacterial community structure in the degenerated soil and the accompanying changes in metabolic function were the important reasons for the inability of the Handkea utriformis continue to grow.
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备注/Memo
第一作者简介：尹淑丽（1979-），女，硕士，研究员，现主要从事食用菌野生资源驯化、栽培育种及农业废弃物食用菌栽培资源利用等研究工作。E-mail:174243369@qq.com.基金项目：河北省科学院重点研发计划资助项目（22314）；河北省科技厅省重点研发计划资助项目（21373804D）。收稿日期：2022-11-21