[1]朱薇,吴志苹,李雪,等.观赏石榴品种的ISSR遗传关系与DNA指纹研究[J].北方园艺,2023,(15):59-65.[doi:10.11937/bfyy.20222865]
　ZHU Wei,WU Zhiping,LI Xue,et al.Study on Genetic Relationship and DNA Fingerprint of Ornamental Pomegranate Cultivars Based on ISSR Markers[J].Northern Horticulture,2023,(15):59-65.[doi:10.11937/bfyy.20222865]
点击复制

观赏石榴品种的ISSR遗传关系与DNA指纹研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2023年15 页码: 59-65 栏目: 出版日期: 2023-08-15
Title:
Study on Genetic Relationship and DNA Fingerprint of Ornamental Pomegranate Cultivars Based on ISSR Markers
作者:
朱薇1; 吴志苹2; 李雪3; 王庆军1; 袁立新2; 马丽2
（1.枣庄市农业农机技术推广中心，山东 枣庄 277800；2.枣庄学院 生命科学学院，山东 枣庄 277160；3.枣庄市农业农村事业发展中心，山东 枣庄 277800）
Author(s):
ZHU Wei1; WU Zhiping2; LI Xue3; WANG Qingjun1; YUAN Lixin2; MA Li2
（1.Zaozhuang Agricultural and Mechanical Technology Promotion Center,Zaozhuang, Shangdong 277800;2.Life Science Department of Zaozhuang College,Zaozhuang,Shangdong 277160;3.Zaozhuang Agricultural and Rural Development Center,Zaozhuang,Shandong 277800）
关键词:
观赏石榴; ISSR标记; 遗传关系; DNA指纹图谱
Keywords:
ornamental pomegranate; ISSR; genetic relationship; DNA fingerprint
DOI:
10.11937/bfyy.20222865
文献标志码:
A
摘要:
以42个观赏石榴品种为试材，采用ISSR标记法，研究了42个品种的遗传关系与指纹图谱，以期为观赏石榴种质资源的品种鉴定、杂交育种和开发利用等提供参考依据。结果表明：16条引物产生327个位点，其中279个是多态位点，平均引物多态性比率为85.32%，说明所选引物多态性较好。42个石榴品种间遗传相似系数(GS)为0.46~0.98，说明观赏石榴品种的遗传多样性较为丰富。当遗传相似系数阈值为0629时，可以将42个品种分为四大类，分类结果与按照形态学分类不一致，与品种地理来源无明显相关性。通过研究引物和引物组合对42个品种的聚类区分率构建DNA指纹，16条单引物的品种区分率为16.67%~88.10%，16条引物均不能将全部品种区分开。引物组合UBC835/UBC827对42个品种的最大遗传相似系数为0.97，品种区分率达100.00%，将引物组合UBC835/UBC827产生的DNA谱带作为42个观赏石榴品种的DNA指纹图谱。
Abstract:
The genetic relationship and DNA fingerprint of 42 ornamental pomegranate cultivars were studied by ISSR markers in this study,in order to provide reference for breeding and utilization of ornamental pomegranate.The results showed that a total of 327 bands were amplified from 16 ISSR primers with good polymorphism，of which 279 were polymorphic loci.The average polymorphic ratio of the primers was 85.32%.The genetic similarity coefficient between 42 cultivars was from 0.46 to 0.98 that showed the genetic diversity of 42 ornamental cultivars was relatively rich.The clustering results by UPGMA showed that 42 cultivars could be divided into four groups at GS 0.629.The clustering results by ISSR marker did not accord with the classification results based on morphological method and geographical origin.The cultivar distinguish rate of 16 primers ranged from 16.67% to 88.10%，so 16 single primers could not distinguish all cultivars.The maximum genetic similarity coefficient (GS) of UBC835/UBC827 combination between cultivars was 0.97 and the distinguish efficiency for 42 cultivars was 100.00%.Therefore the DNA bands form primer combination UBC835/UBC827 were used to construct the DNA fingerprint of 42 ornamental pomegranate cultivars.

参考文献/References:

[1]马丽,侯乐峰,郝兆祥,等.82个石榴品种遗传多样性的ISSR分析[J].果树学报,2015，32(5):741-750.[2]尹燕雷,苑兆和,冯立娟,等.我国石榴种质资源研究进展[J].山东林业科技,2008，176(3)：80-99.[3]侯乐峰,耿道鹏,苏成.峄城石榴种质资源研究[J].落叶果树,2006(5)：19-21.[4]热娜·卡司木,帕丽达·阿不力孜,朱焱.新疆石榴品种的RAPD分析[J].西北植物学报，2008，28(12):2447-2450.[5]杨荣萍,龙雯虹,张宏,等.云南25份石榴资源的RAPD分析[J].果树学报,2007(2):226-229.[6]王庆军,罗华,赵丽娜,等.24个山东石榴品种遗传多样性的ISSR分析[J].中国果树,2018(2):18-22.[7]黄平.月季品种分子鉴定与遗传关系分析[D].北京：中国林业科学研究院,2012.[8]刘倩，戴志刚，陈基权,等.应用SRAP分子标记构建红麻种质资源分子身份证[J].中国农业科学，2013，46(10):1974-1983.[9]吴婷,魏珊,米丽华,等.不同产地连翘的DNA指纹图谱构建与聚类分析[J].中草药，2016,47(5):816-820.[10]苑兆和,招雪晴.石榴种质资源研究进展[J].林业科技开发,2014,28(3):1-7.[11]赵丽华,李名扬,王先磊.川滇石榴品种遗传多样性及亲缘关系的AFLP分析[J].林业科学,2010,46(11):168-173.[12]陈芸,王继莲,丁晓丽,等.新疆石榴种质资源遗传多样性的SRAP分析[J].西北植物学报,2016,36(5):916-922.[13]宣继萍,章镇,房经贵,等.苹果品种ISSR指纹图谱构建[J].果树学报,2002(6):421-423.[14]华树妹,贺佩珍,陈芝华,等.应用SRAP标记构建山药种质资源DNA指纹图谱[J].植物遗传资源学报,2014,15(3):597-603.[15]郑志雷.厚朴遗传多样性研究及指纹图谱的构建[D].福州:福建农林大学,2010.[16]汪斌,祁伟,兰涛,等.应用ISSR分子标记绘制红麻种质资源DNA指纹图谱[J].作物学报,2011(6):186-193.[17]王壮伟,赵密珍,袁骥,等.38个欧美草莓栽培品种SSR指纹图谱的构建[J].果树学报,2011,28(6):1032-1037.[18]沈进.中国石榴资源初级核心种质构建及其ISSR分子标记研究[D].合肥:安徽农业大学,2008.

相似文献/References:

[1]尹光晶,宋廷宇,吴春燕,等.豇豆ISSR-PCR反应体系的建立[J].北方园艺,2012,36(12):141.
　YINGuang-jing,SONGTing-yu,WUChun-yan,et al.EstablishmentofInterSimpleSequenceRepeatPCRReactionSysteminAsparagusBean[J].Northern Horticulture,2012,36(15):141.
[2]颜秀娟,何鑫,王学梅,等.宁夏羊角椒雄性不育基因ISSR的分子标记[J].北方园艺,2015,39(17):82.[doi:10.11937/bfyy.201517021]
　YAN Xiujuan,HE Xin,WANG Xuemei,et al.ISSR Marker of Male Sterility in Pepper of Ningxia[J].Northern Horticulture,2015,39(15):82.[doi:10.11937/bfyy.201517021]
[3]张妍,邬向丽,黄晨阳,等.金顶侧耳种质资源的遗传多样性鉴定及农艺性状评价[J].北方园艺,2018,42(11):151.[doi:10.11937/bfyy.20174741]
　ZHANG Yan,WU Xiangli,HUANG Chenyang,et al.Identification of Genetic Diversity and Evaluation of Agromomic Traits for the Germplasm Resources of Pleurotus citrinopileatus[J].Northern Horticulture,2018,42(15):151.[doi:10.11937/bfyy.20174741]
[4]李慧,夏伟伟,胡子钰,等.利用ISSR-SCAR标记鉴定金针菇双-单杂交子[J].北方园艺,2022,(16):121.[doi:10.11937/bfyy.20220445]
　LI Hui,XIA Weiwei,HU Ziyu,et al.Identification of Dikaryon-monokaryon Hybrid of Flammulina filiformis by ISSR-SCAR Marker[J].Northern Horticulture,2022,(15):121.[doi:10.11937/bfyy.20220445]
[5]焦枥禾,马银鹏,王凤利,等.无菌水长期保藏黑木耳菌种性状研究[J].北方园艺,2023,(12):116.[doi:10.11937/bfyy.20224093]
　JIAO Lihe,MA Yinpeng,WANG Fengli,et al.Study on Properties of Auricularia heimuer Preserved inSterile Water for Long Time[J].Northern Horticulture,2023,(15):116.[doi:10.11937/bfyy.20224093]

备注/Memo

第一作者简介：朱薇（1990-），女，硕士，农艺师，现主要从事农业技术研究与推广等工作。Email：18263265679@163.com.责任作者：马丽（1980-），女，博士，副教授，现主要从事分子标记辅助石榴种质资源育种等研究工作。Email：mary1976816@163.com.基金项目：国家大学生创新创业训练资助项目（202210904001）；山东省高等学校科技计划资助项目（J14LF02）；山东省农业科技发展资金资助项目（2015）；枣庄英才集聚工程资助项目（枣政办字[2016]23号）;枣庄市科技发展计划资助项目（2021NS21）。收稿日期：2022-07-12

更新日期/Last Update: 2023-09-19