HUANG Xingmin,DENG Xiaohong,PENG Hailan,et al.Effects of Elevated CO2 Concentration and Nitrogen Deposition on the Growth and Its Medicinal Components of Reineckia carnea Kunth[J].Northern Horticulture,2023,(06):104-111.[doi:10.11937/bfyy.20222659]
CO2浓度升高和氮沉降对吉祥草生长及品质的影响
- Title:
- Effects of Elevated CO2 Concentration and Nitrogen Deposition on the Growth and Its Medicinal Components of Reineckia carnea Kunth
- 文献标志码:
- A
- 摘要:
- 以苗族常用药材吉祥草 （Reineckia carnea（Andr.） Kunth）为试材，采用盆栽控制试验，设置了对照(CK)、单独增二氧化碳(eC：CO2浓度为（800±20） μmol?mol-1)、单独施氮(eN：施加5 g NH4NO3)、同时增二氧化碳与施氮(eC+eN：CO2浓度为（800±20） μmol?mol-1，且施加5 g NH4NO3) 4个处理，研究CO2浓度升高、氮沉降及二者的交互作用对吉祥草的生长及其药用成分的影响，以期为吉祥草在药用生产上提供参考依据。结果表明：1）升高CO2浓度和施氮或同时升高CO2浓度和施氮均显著促进了吉祥草的形态生长，总生物量和可溶性蛋白质含量显著增加，茎中的碳含量显著降低，超氧化物歧化酶（SOD）活性无显著变化；2）施氮处理下全氮含量显著增加，过氧化氢酶（CAT）活性、根和叶中的皂苷含量显著下降，在此基础上升高CO2浓度可缓解皂苷下降，CAT活性显著升高，内源激素含量在施氮或同时升高CO2浓度和施氮处理下显著增加。综上所述，在实际生产过程中，可适当增加CO2浓度，氮肥浓度不宜过高，有利于吉祥草的生长，并保证其药用品质不受损害。
- Abstract:
- Taking Reineckia carnea（Andr.）Kunth,a commonly used medicinal herb of the Miao nationality,as the research object,the pot control experiment was carried out,and four treatments were set up,including control (CK),carbon dioxide increase alone (eC:CO2 concentration ((800±20)μmol ? mol-1),nitrogen application alone (eN: application of 5 g NH4NO3),carbon dioxide increase and nitrogen application simultaneously (eC+eN:CO2 concentration (800±20)μmol ? mol-1,and application of 5 g NH4NO3),the effects of elevated CO2 concentration,nitrogen deposition and their interaction on the growth and medicinal components of Reineckia carnea（Andr.） Kunth were studied,in order to provide reference for the medicinal production of Reineckia carnea（Andr.） Kunth.The results showed that,1) increasing CO2 concentration and nitrogen application or simultaneously increasing CO2 concentration and nitrogen application significantly promoted the morphological growth,total biomass and soluble protein content significantly increased,carbon content in stem significantly decreased,and super oxide dismuta (SOD) activity had no significant change.2) Under nitrogen application,the total nitrogen content increased significantly,Catalase (CAT) activity and saponin content in roots and leaves decreased significantly.On this basis,increasing CO2concentration could alleviate the decline of saponin,CAT activity increased significantly,and endogenous hormone content increased significantly under nitrogen application or simultaneously increasing CO2 concentration and nitrogen application.In the actual production process,the concentration of CO2 can be appropriately increased,but the concentration of nitrogen should not be too high,which is beneficial to the growth of Reineckia carnea Kunth and ensure its medicinal quality is not damaged.
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备注/Memo
第一作者简介：黄兴敏（1997-），女，硕士研究生，研究方向为植物生态学。E-mail:2049550554@qq.com.责任作者：王健健（1985-），男，博士，副教授，现主要从事植物生态学等研究工作。E-mail：wangjj33209@163.com.基金项目：国家自然科学基金资助项目（31760155）；贵州省生物学一流学科建设资助项目（GNYL[2017]009）。收稿日期：2022-06-28