CHEN Xiaojiong,YANG Xinpeng,MA Bingjin,et al.Optimization of Subcritical Water Extraction of Total Flavonoids From Sonchus arvensis L.and Evaluation of Its Antioxidant Activity[J].Northern Horticulture,2022,(13):105-111.[doi:10.11937/bfyy.20220297]
亚临界水提取苣荬菜总黄酮的工艺优化及其抗氧化活性研究
- Title:
- Optimization of Subcritical Water Extraction of Total Flavonoids From Sonchus arvensis L.and Evaluation of Its Antioxidant Activity
- Keywords:
- Sonchus arvensis L.; total flavonoids; subcritical water extraction; response surface methodology; antioxidant activity
- 文献标志码:
- A
- 摘要:
- 以苣荬菜为试材，采用单因素试验结合响应面法优化亚临界水提取总黄酮的工艺，研究了总黄酮提取物的抗氧化活性，以期为苣荬菜的进一步开发利用提供参考依据。结果表明：苣荬菜总黄酮最佳提取工艺条件为提取温度127 ℃、提取时间16 min、液料比24∶1 mL?g-1、搅拌速率120 r?min-1，在该条件下总黄酮提取量为39.02 mg?g-1，与预测值39.06 mg?g-1高度吻合，表明该提取工艺稳定可行。在最佳工艺条件下得到的苣荬菜总黄酮提取物具有较好的Fe2+螯合能力和Fe3+还原能力，表明苣荬菜可作为天然抗氧化剂的原料来源。
- Abstract:
- Taking the Sonchus arvensis L.as test material，the process of subcritical water extraction of total flavonoids was optimized by one-factor test combined with response surface methodology，and the antioxidant activity of crude flavonoids extracts was also evaluated,in order to provide reference for the further development and utilization of S.arvensis L..The results showed that the optimal extraction conditions were extraction temperature 127 ℃，extraction time 16 minutes，liquid-solid ratio 24∶1 mL?g-1，and the stirring rate 120 r?min-1.Under this condition，the total flavonoids yield was 39.02 mg?g-1，which was highly consistent with the predicted value of 39.06 mg?g-1，indicating that the extraction process was stable and feasible.Crude total flavonoids extracts obtained under the optimal conditions showed good Fe2+ chelating capacity and Fe3+ reducing capacity，indicating that S.arvensis L. could be used as a material source of natural antioxidants.
参考文献/References:
[1]林余霖，李葆莉.新编中草药全图鉴[M].福州：福建科学技术出版社，2020.[2]董庆海，李雅萌，吴福林，等.苣荬菜的研究进展[J].特产研究，2018，40(3)：75-78.[3]KHAN R A.Evaluation of flavonoids and diverse antioxidant activities of Sonchus arvensis[J].Chemistry Central Journal，2012，6(1)：1-7.[4]YIN J，SI C L，WANG M H.Antioxidant activity of flavonoids and their glucosides from Sonchus oleraceus L[J].Journal of Applied Biological Chemistry，2008，51(2)：57-60.[5]王丹.脱脂豆粕中大豆异黄酮提取工艺的研究[J].食品研究与开发，2017，38(6)：52-55.[6]杨爽，周仕强，刘影，等.正交试验法优化索氏提取薏米中黄酮[J].粮食与油脂，2021，34(9)：126-129.[7]HOU M，HU W，WANG A，et al.Ultrasound-assisted extraction of total flavonoids from Pteris cretica L.：Process optimization，HPLC analysis，and evaluation of antioxidant activity[J].Antioxidants，2019，8(10)：425.[8]ALARA O R，ABDURAHMAN N H，OLALERE O A.Optimization of microwave-assisted extraction of flavonoids and antioxidants from Vernonia amygdalina leaf using response surface methodology[J].Food and Bioproducts Processing，2018，107：36-48.[9]滕艾静，李宇飞，郭东会，等.苣荬菜中黄酮提取工艺改进及其抑菌分析[J].泰山医学院学报，2020，41(7)：531-534.[10]徐清，姜笑寒，邱建波.正交优选苣荬菜中总黄酮的提取工艺[J].现代医药卫生，2011，27(7)：993-994.[11]ZHENG P，HAO G，WENG W，et al.Antioxidant activities of hydrolysates from abalone viscera using subcritical water-assisted enzymatic hydrolysis[J].Food and Bioprocess Technology，2019，12(6)：910-918.[12]奉建芳，毛声俊，冯年平，等.现代中药制剂设计[M].北京：中国医药科技出版社，2020.[13]KO M J，CHEIGH C I，CHUNG M S.Relationship analysis between flavonoids structure and subcritical water extraction (SWE)[J].Food Chemistry，2014，143：147-155.[14]KO M J，KWON H L，CHUNG M S.Pilot-scale subcritical water extraction of flavonoids from satsuma mandarin (Citrus unshiu Markovich) peel[J].Innovative Food Science & Emerging Technologies，2016，38：175-181.[15]KIM D S，LIM S B.Kinetic study of subcritical water extraction of flavonoids from Citrus unshiu peel[J].Separation and Purification Technology，2020，250：117259.[16]侯梦阳，胡文忠，修志龙，等.大孔吸附树脂富集纯化玳玳花总黄酮[J].中成药，2021，43(1)：16-21.[17]HUANG C Y，WU S J，YANG W N，et al.Antioxidant activities of crude extracts of fucoidan extracted from Sargassum glaucescens by a compressional-puffing-hydrothermal extraction process[J].Food Chemistry，2016，197：1121-1129.[18]YAO X H，ZHANG D Y，ZU Y G，et al.Free radical scavenging capability，antioxidant activity and chemical constituents of Pyrola incarnata Fisch.leaves[J].Industrial Crops and Products，2013，49：247-255.[19]周丽，张博雅，张永忠.亚临界水提取葛根中总异黄酮的研究[J].中草药，2012，43(3)：492-495.[20]闫林林,郑光耀,韦朝宽.响应面法优化石榴叶总酚的亚临界水提取工艺[J].食品工业科技，2017，38(19)：184-190.[21]汪泽，崔丽虹，唐冰，等.响应面法优化菠萝叶纤维多酚提取工艺[J].食品工业科技，2017，38(1)：219-223.[22]李艳艳，王倩倩，苏圆圆，等.响应面法优化提取曼地亚红豆杉总黄酮及抗氧化活性[J].北方园艺，2021(5)：94-102.[23]郝可欣，胡文忠，张清洁，等.响应面法优化超声辅助提取代代花总黄酮的工艺及其抗氧化活性研究[J].食品工业科技，2019，40(24)：159-164,171.
相似文献/References:
[1]张 虹,王景李,白红丽,等.云南翠雀花根中总黄酮提取方法的研究[J].北方园艺,2014,38(13):153.
ZHANG Hong,WANG Jing-li,BAI Hong-li,et al.Study on the Extraction Method Total Flavonoids in Root of the Delphinium yunnanense[J].Northern Horticulture,2014,38(13):153.
[2]杨申明,王振吉,沈继森.彝药猪鬃草中总黄酮提取工艺研究[J].北方园艺,2014,38(11):125.
YANG Shen-ming,WANG Zhen-ji,SHEN Ji-sen.Study of the Extraction Technology of Total Flavonoids in Adiantum capillus-veneris[J].Northern Horticulture,2014,38(13):125.
[3]王亚红,王亚丽,祝 波.瓦松总黄酮的索氏提取工艺研究[J].北方园艺,2014,38(10):130.
WANG Ya-hong,WANG Ya-li,ZHU Bo.Study on Soxhlet Extraction Technology of Total Flavonoids From Orostachys fimbriatus[J].Northern Horticulture,2014,38(13):130.
[4]段晓琴.三种保健野菜的营养成分测定[J].北方园艺,2013,37(17):42.
DUAN Xiao-qin.Determination of Nutritious Components in Three Kinds of Edible Wild Herbs[J].Northern Horticulture,2013,37(13):42.
[5]韩凤波,奚广生.不同采收期对轮叶党参有效成分含量的影响[J].北方园艺,2014,38(01):154.
HAN Feng-bo,XI Guang-sheng.Effect of Different Harvest Time on the Effective Component Content of Codonopsis lanceolata [J].Northern Horticulture,2014,38(13):154.
[6]闵莉静,李敬芬,赵明星.超声辅助提取橘皮总黄酮的工艺优化研究[J].北方园艺,2013,37(13):172.
MIN Li-jing,LI Jing-fen,ZHAO Ming-xing.Study on Optimization of Ultrasonic Assisted Extraction Technology of ?Total Flavonoids from Orange Peel[J].Northern Horticulture,2013,37(13):172.
[7]韩凤波,陈静雅.不同采收期对香椿总黄酮和总皂苷含量的影响[J].北方园艺,2014,38(02):159.
HAN Feng-bo,CHEN Jing-ya.Effect of Harvest Season on Total Saponins Content and Total Flavone Content of Toona sinensis [J].Northern Horticulture,2014,38(13):159.
[8]任吉君,王艳,肖华凤.香薷总黄酮萃取工艺参数优化研究[J].北方园艺,2013,37(14):140.
REN Ji-jun,WANG Yan,XIAO Hua-feng.Study on Optimized Extraction of Total Flavones from Elsholtzia ciliata Thunb.[J].Northern Horticulture,2013,37(13):140.
[9]王晓洁,盛东峰.不同溶剂对女贞子黄酮提取效果和抑菌活性的影响[J].北方园艺,2014,38(09):140.
WANG Xiao-jie,SHENG Dong-feng.Effect of Different Solvents on Extraction of Effective Total Flavones and Antibacterial Activity of Ligustrum lucidum [J].Northern Horticulture,2014,38(13):140.
[10]杨艳俊,王亚红,王君龙.景天三七总黄酮大孔吸附树脂纯化工艺研究[J].北方园艺,2014,38(07):144.
YANG Yan-jun,WANG Ya-hong,WANG Jun-long.Study on the Purification Process of Total Flavonoids of Sedum aizoon L. by Macroporous Adsorption Resin[J].Northern Horticulture,2014,38(13):144.
备注/Memo
第一作者简介：陈晓炯（1979-），男，本科，工程师，现主要从事林业资源保护与可持续开发等研究工作。E-mail：qlsedu@sohu.com.责任作者：杨新鹏（1980-），男，本科，工程师，现主要从事林业资源保护与可持续开发等研究工作。E-mail：northwestedu@126.com.基金项目：甘肃省科技计划资助项目（21CX6NH283）。收稿日期：2022-01-21