[1]赖晓辉,杨晓绒,张相锋.郁金香连作土壤微生物多样性分析[J].北方园艺,2022,(11):75-82.[doi:10.11937/bfyy.20214626]
　LAI Xiaohui,YANG Xiaorong,ZHANG Xiangfeng.Diversity Analysis of Microorganism in Tulip Continuous Cropping Soil[J].Northern Horticulture,2022,(11):75-82.[doi:10.11937/bfyy.20214626]
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郁金香连作土壤微生物多样性分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2022年11 页码: 75-82 栏目: 出版日期: 2022-06-15
Title:
Diversity Analysis of Microorganism in Tulip Continuous Cropping Soil
作者:
赖晓辉1; 杨晓绒1; 张相锋1; 2
（1.伊犁师范大学 生物与地理科学学院,新疆 伊宁 835000;2.伊犁师范大学 微生物资源保护与开发利用重点实验室,新疆 伊宁 835000）
Author(s):
LAI Xiaohui1; YANG Xiaorong1; ZHANG Xiangfeng1; 2
(1.College of Biological and Geographical Science,Yili Normal University,Yining,Xinjiang 835000;2.Key Laboratory of Microbial Resources Protection,Development and Utilization,Yili Normal University,Yining,Xinjiang 835000)
关键词:
高通量测序; 郁金香; 连作; 土壤微生物多样性
Keywords:
high-throughput sequencing; tulip; continuous cropping; soil microbial diversity
DOI:
10.11937/bfyy.20214626
文献标志码:
A
摘要:
以新疆伊犁昭苏县郁金香种球连作土壤为试验材料，采用高通量测序技术（Illumina Miseq 2x300 bp）和生物信息学分析方法，研究不同连作年限土壤微生物多样性，探究土壤微生物动态变化对郁金香种球连作障碍的影响，以期为有效预防郁金香连作障碍提供参考依据。结果表明：在不同连作年限的15份土样中，细菌共有2 305个OTU分类单元，主要隶属于35门317属，真菌共有831个OTU分类单元，主要隶属于9门231属；土壤微生物多样性会随连作年限呈下降趋势，物种相对丰度也会随连作年限发生显著变化。
Abstract:
Taking tulip continuous cropping in Zhaosu county,Yili,Xinjiang as the test material,the high-throughput sequencing (Illumina Miseq 2x300 bp) and bioinformatics analysis method were used,the soil microbial diversity of tulip continuous cropping was studied,the effect of soil microbial dynamics on continuous cropping barriers of tulip was explored,in order to provide reference for the effective preventing of tulip continuous cropping obstacles. The results showed that bacteria mainly belonged to 317 genera of 35 phyla and fungi mainly belonged to 231 genera of 9 phyla in 15 soil samples of 5 different continuous cropping years.Soil microbial diversity decreased with continuous cropping years,and the relative abundance of species changed significantly with continuous cropping years.

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备注/Memo

第一作者简介：赖晓辉（1989-），女，硕士，讲师，现主要从事植物逆境分子生物学等研究工作。E-mail：327060649@qq.com.责任作者：张相锋（1976-），男，硕士，副教授，现主要从事微生物促生机理等研究工作。E-mail：759737497@qq.com.基金项目：伊犁师范大学微生物资源保护与开发利用重点实验室科研资助项目（YLUKLM2018007）。收稿日期：2021-11-14

更新日期/Last Update: 2022-07-19