WANG Zheng,SUN Yuke,SHANG Wenqian,et al.Effects of Different Illumination Methods of LED on Growth of Lilium brownii in vitro[J].Northern Horticulture,2022,(06):70-76.[doi:10.11937/bfyy.20214284]
LED不同照光方式对百合组培苗生长的影响
- Title:
- Effects of Different Illumination Methods of LED on Growth of Lilium brownii in vitro
- Keywords:
- LED; Lilium brownii; lateral illumination; reflective processing; tissue culture
- 文献标志码:
- A
- 摘要:
- 以‘索尔邦’百合为试材,采用不同倾斜角度的LED侧向照光和顶部照光2种照光方式,结合不同造型曲面反光膜和平面反光膜,研究8种不同组合LED照光方式对百合组培苗生长的影响,以期为百合组培及工厂化育苗提供参考依据。结果表明:8种处理中,LED不同照光方式处理的百合组培苗,其形态和生理指标整体优于对照处理。其中,采用倾斜69.77°侧向照光方式和平面反光膜的处理5更有利于百合组培苗茎叶的生长,其在叶幅、地上鲜质量、地上干质量、叶绿素b含量、叶绿素总含量等方面高于其它处理;而在根系发育方面,采用倾斜79.89°侧向照光方式和曲面反光膜的处理2百合组培苗整体优于其它处理,其根数、根鲜质量、根干质量、根系活力等多个指标显著高于其它处理。因此,采用倾斜69.77°侧向照光方式和平面反光膜的处理5在促进百合组培苗茎叶生长方面效果最佳,采用倾斜79.89°侧向照光方式和曲面反光膜的处理2有利于百合组培苗根系生长。
- Abstract:
- The ‘Solbourne’ was used as the test material,two illumination modes of LED side illumination and top illumination with different inclined angles were adopted,combined with different shapes of curved reflective film and flat reflective film to clarify the effects of eight different combinations of LED lighting methods on the growth of lilies in vitro,in order to provide reference for lilies in vitro and factory seedling improvement.The results showed that eight kinds of different illumination methods of lilies in vitro on morphology and physiology were better than those of the control treatment.Among them,the treatment 5 with oblique 69.77° lateral illumination and plane reflective film was more conducive to the growth of lilies in vitro on stem and leaf growth.Its leaf width,fresh weight of aerial parts,dry weight of aerial parts,chlorophyll b content,total chlorophyll content were higher than other treatments.In terms of root development,treatment 2′s lilies in vitro with oblique 79.89° lateral illumination and curved reflective film was overall better than the other.Its root number,fresh weight of root,dry weight of root,root activity,and many other indicators were significantly higher than other treatments.Therefore,treatment 5 with oblique 69.77° lateral illumination and plane reflective film had the best effect in promoting stem and leaf growth of lilies in vitro,and treatment 2 with oblique 79.89° lateral illumination and curved reflective film was beneficial to root growth.
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备注/Memo
第一作者简介:王政(1980-),男,博士,副教授,现主要从事园林植物栽培生理与生物技术等研究工作。E-mail:wzhengt@163.com.责任作者:何松林(1965-),男,博士,教授,博士生导师,现主要从事园林植物栽培生理与生物技术等研究工作。E-mail:hsl213@yeah.net.基金项目:河南省产学研资助项目(162107000068)。收稿日期:2021-10-22