LIANG Juan,CHEN Duanfen.Effects of Light Quality and Light Intensity on ‘Blue Mouse Ears’ Hosta Tissue Culture Plantlets[J].Northern Horticulture,2022,(08):52-59.[doi:10.11937/bfyy.20214079]
光质和光强对‘蓝鼠耳’玉簪试管苗的影响
- Title:
- Effects of Light Quality and Light Intensity on ‘Blue Mouse Ears’ Hosta Tissue Culture Plantlets
- Keywords:
- Hosta; light quality; light intensity; proliferation rate; rooting rate
- 文献标志码:
- A
- 摘要:
- 以‘蓝鼠耳’玉簪为试材，利用LED光源，研究了不同光质和光强对试管苗生长及生根的影响，以期为‘蓝鼠耳’玉簪试管苗的工厂化生产提供参考依据。结果表明：1）LED光质为W∶R=7∶1时，试管苗的增殖系数为4.71，是所有处理中的最大值；株高和叶面积综合表现良好，分别为17.48 mm和30.90 mm2，说明W∶R=7∶1时对试管苗的生长有较好的促进作用。试管苗的生根率在R∶B=5∶1、W∶R=7∶1、R∶W=2∶3时均高于97%，优于其它处理。LED光质为R∶W=2∶3时试管苗的根数为17.54条，说明光质R∶W=2∶3时能够有效促进试管苗根系生长。2）低光强59 μmol?m-2?s-1〖JP2〗时有利于试管苗增殖，随着光照强度的增加，增殖系数呈下降趋势。当光强为176~235 μmol?m-2?s-1时，试管苗的叶面积表现良好，为73.09 mm2，说明该光强对试管苗的生长有较好的促进作用。当光照强度为118~176 μmol?m-2?s-1时试管苗的最大根长为7.44 mm，说明该光强可以促进试管苗根系的生长。最低光强（0~59 μmol?m-2?s-1）和最高光强（706~824 μmol?m-2?s-1）不利于试管苗的生长。
- Abstract:
- ‘Blue Mouse Ears’ Hosta was used as the research material.The effects of different light quality and light intensity on the growth and rooting of tissue culture plantlets were studied by LED,the suitable light quality and light intensity were selected,in order to provide reference for the industrialized production of ‘Blue Mouse Ears’ Hosta plantlets.The results showed that,1）when the LED light quality was W∶R=7∶1,the proliferation rate of tissue culture plantlets was 4.71,which was the highest among all treatments;the comprehensive performance of plant height and leaf area was good,which were 17.48 mm and 30.90 mm2 respectively,indicated that W∶R=7∶1 could promote the growth of tissue culture plantlets.Treatments of R∶B=5∶1,W∶R=7∶1 and R∶W=2∶3 had a rooting rate higher than 97%,which were better than other treatments.When the LED light quality was R∶W=2∶3,the root number of plantlets was 17.54,which indicated that the light quality R∶W=2∶3 could promote the root growth of tissue culture plantlets effectively.2）The lowest light intensity 59 μmol?m-2?s-1 was beneficial to the proliferation of tissue culture plantlets,with the increase of light intensity,the proliferation coefficient decreased.When the light intensity was 176-235 μmol?m-2?s-1,the leaf area of tissue culture plantlets were good,and the numerical value was 73.09 mm2 respectively,indicated that the light intensity of 176-235 μmol?m-2?s-1 could promote the growth of tissue culture plantlets.When the light intensity was 118-176 μmol?m-2?s-1,the maximum root length was 7.44 mm,which indicated that the light intensity in the range of 118-176 μmol?m-2?s-1 could promote the root growth of tissue culture plantlets.The lowest light intensity (0-59 μmol?m-2?s-1) and the highest light intensity (706-824 μmol?m-2?s-1) were not conducive to the growth of plantlets.
参考文献/References:
[1]贾洪革.玉簪品种的引种,栽培和组织培养[D].北京:中国科学院植物研究所,2000.[2]徐庆祥.玉簪属植物生理生态学研究进展[J].安徽农业科学,2017,45(15):6-8,13.[3]de CAPITE L.Action of light and temperature on growth of plant tissue cultures in vitro[J].American Journal of Botany，1955，42(10):869-873.[4]李杰,王再花,刘海林,等.不同光质的LED对2种金线莲组培苗增殖、生根及生长的影响[J].热带作物学报,2017,38(9):1666-1670.[5]王亚沉,王玉英,徐诗涛,等.LED不同光质对碧玉兰组培苗增殖的影响[J].安徽农业科学,2015,43(22):3-4,16.[6]任桂萍,王小菁,朱根发.不同光质的LED对蝴蝶兰组织培养增殖及生根的影响[J].植物学报,2016,51(1):81-88.[7]杨锁宁,申雯靖,张黎.不同光质比对金钻蔓绿绒试管苗形态建成的影响[J].分子植物育种,2018,16(21):7171-7178.[8]刘梦溪.LED光谱对桔梗试管苗形态建成及离体成花的影响[D].南京:南京农业大学,2014.[9]王政,马杰,何松林,等.LED光源不同比例光质对红叶石楠试管苗生长的影响[J].江苏农业科学,2019,47(7):152-155.[10]庹晓庆,孙翊,殷丽青,等.LED光质对矾根‘红贝露’增殖培养的影响[J].分子植物育种,2020,18(20):6793-6799.[11]POUDEL P R,KATAOKA I,MOCHIOKA R,et al Effects of light quality on growth and development of protocorm-like bodies of Dendrobium officinale in vitro[J].Plant Cell,Tissue and Organ Culture (Pctoc),2011,105(3):329-335.[12]KONG S S,MURTHY H N,HEO J W,et al.The effect of light quality on the growth and development of in vitro cultured Doritaenopsis plants[J].Acta Physiologiae Plantarum,2008,30(3)339-343.[13]朱秀蕾.LED光质对白芨组培苗生长的影响[J].黔南民族师范学院学报,2019,39(4):22-25.[14]仇学文,许建民,史金兰,等.光质对康乃馨组培苗生长的影响[J].安徽农学通报,2018,24(9):45-47.[15]杨晓芬,张京伟,张金政,等.LED光源对玉簪试管苗增殖生长及光合特性的影响[J].上海交通大学学报(农业科学版),2018,36(6):50-54.[16]贾文庆,郭英姿,刘会超,等.不同光质对牡丹杂种胚组培苗生根的影响[J].林业科技通讯,2017(9):27-29.[17]李胜,李唯,杨德龙,等.不同光质对葡萄试管苗根系生长的影响[J].园艺学报,2005(5):107-109.[18]李韶山,潘瑞炽.植物的蓝光效应[J].植物生理学通讯,1993,29(4):248-252.[19]刘伟超,王政,何松林,等.不同红蓝光质比对大花萱草组培苗增殖及生根的影响[J].西北林学院学报,2019,34(2):134-139.[20]骆娜,张小霞,杨丽娇,等.不同光质对珊瑚姜组培苗生长的影响[J].热带作物学报,2018,39(4):651-655.[21]肖林霞,卢其能,李润根.光质、光强和外植体对虎杖愈伤组织增殖及白藜芦醇积累的影响[J].江苏农业科学,2016,44(11):60-63.[22]覃婕,黄钊.LED组合光源对红掌愈伤组织培养的影响[J].农业与技术,2017,37(4):32.[23]王政,刘艺平,张春丽,等.LED不同光照度对大花蕙兰试管苗生长和叶片气孔特征的影响[M]//张启翔.中国观赏园艺研究进展(2013).北京：中国农业出版社，2013.[24]王萌,王政,何松林,等.光照强度对菊花试管苗生长和抗氧化酶活性的影响[J].西北农林科技大学学报(自然科学版),2020,48(11):71-76,86.[25]尹明华,王艾平.光照时间和光照强度对红芽芋试管苗生长发育的影响[J].贵州农业科学,2013,41(9):63-65.[26]尚文倩,王政,何松林,等.不同红蓝光质比和光照强度对金娃娃萱草试管苗生长的影响[J].西北农林科技大学学报(自然科学版),2017,45(7):90-96.[27]杨艳琼,王荔,陈疏影,等.不同光照强度对灯盏花无糖组培苗生长发育的影响[J].云南农业大学学报,2007(3):323-326.
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备注/Memo
第一作者简介：梁娟（1992-)，女，硕士，助理工程师，现主要从事植物组织培养等研究工作。E-mail:1239834421@qq.com.责任作者：陈段芬（1968-），女，博士，教授，现主要从事观赏植物生产与应用等研究工作。E-mail：chenduanfen@163.com.收稿日期：2021-10-05