WANG Yiqing,WANG Anqi,FENG Junting,et al.Analysis of Gene Expression and Metabolism Related to Sugar Metabolism of Lilium Asiatica Hybrida[J].Northern Horticulture,2022,(06):60-69.[doi:10.11937/bfyy.20213255]
亚洲百合糖代谢相关基因的表达与代谢分析
- Title:
- Analysis of Gene Expression and Metabolism Related to Sugar Metabolism of Lilium Asiatica Hybrida
- 文献标志码:
- A
- 摘要:
- 以亚洲百合‘蜂鸣’为试材，采用转录组测序、荧光定量PCR技术以及测量种球的表型和生理指标的方法，研究了亚洲百合种球膨大过程中的生理变化及种球膨大前后共11个与种球膨大相关基因的表达量，以期了解亚洲百合糖代谢相关基因及糖代谢途径。结果表明：亚洲百合种球膨大过程中过氧化氢酶（CAT）活性和过氧化物酶（POD）活性显著提高，尤其在根系发育中起重要作用的POD，活性也明显高于CAT；参与蔗糖代谢的可溶性酸性转化酶(SAI)、己糖激酶(HK)、蔗糖磷酸合成酶(SPS)、蔗糖合成酶(SS)，负责淀粉合成的结合态淀粉合成酶(GBSS)和可溶性淀粉合成酶(SSS)活性均有显著增长且变化幅度相似。同时发掘到了与百合种球膨大相关酶8个，分别为CAT、POD、GBSS、SSS、SAI、HK、SPS、SS；相关基因11个，分别为LaSPS、LaSS、LaGBSS、[STBX]LaGBE1[STBZ]、LaUGP、LaAGPase、[STBX]LaGSK-3β[STBZ]、LaCAT、LaPOD、LaSAI、LaHK基因；相关代谢物4个，α-D-甘露糖1-磷酸、2-异丙基苹果酸、熊果苷和L-丝氨酸。
- Abstract:
- Lilium Asiatica hybrida ‘buzzer’ was used as the experimental material.Transcriptome sequencing，qPCR and measurement of the phenotype and the physiological indicators of the bulb were used to study the expression levels of 11 genes related to bulb expansion before and after bulb enlargement，in order to understand the glucose metabolism related genes and glucose metabolism pathway of Lilium Asiatica hybrida.The results showed that the activities of catalase and peroxidase were significantly increased during the bulb enlargement of Lilium Asiatica hybrida，especially the peroxidase，which played an important role in root development，was significantly higher than that of catalase.The activities of soluble acid invertase，hexokinase，sucrose phosphate synthase，sucrose synthase，starch synthase，and soluble amylase，which were involved in sucrose metabolism，increased significantly and changed in a similar range.At the same time，8 enzymes related to bulb expansion were found，including CAT，POD，GBSS，SSS，SAI，HK，SPS，and SS.There were 11 related genes，included LaSPS，LaSS，LaGBSS，[STBX]LaGBE1[STBZ]，LaUGP，LaAGPase，[STBX]LaGSK-3β[STBZ]，LaCAT，LaPOD，LaSAI and LaHK genes.Four related metabolites were α-D-mannose 1-phosphate，2-isopropyl malic acid，arbutin，and L-serine.
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备注/Memo
第一作者简介：王一清（1999-），女，硕士研究生，研究方向为园林植物与观赏园艺。E-mail：954512630@qq.com.责任作者：崔金腾（1982-），男，硕士，高级实验师，现主要从事园林植物与观赏园艺等研究工作。E-mail：cuijinteng@163.com.基金项目：北京市自然科学基金-市教委联合资助项目(KZ2018100200-29)。收稿日期：2021-08-08