[1]玉赛赛,李莎莎,骆强伟,等.胚挽救创造无核抗寒葡萄新种质[J].北方园艺,2021,(22):29-37.[doi:10.11937/bfyy.20212010]
　YU Saisai,LI Shasha,LUO Qiangwei,et al.Embryo Rescue Technique Create New Seedless，Cold-resistant Grapevines Germplasm[J].Northern Horticulture,2021,(22):29-37.[doi:10.11937/bfyy.20212010]

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胚挽救创造无核抗寒葡萄新种质

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2021年22 页码: 29-37 栏目: 出版日期: 2021-11-30

Title:: Embryo Rescue Technique Create New Seedless，Cold-resistant Grapevines Germplasm

作者:: 玉赛赛1; 2; 3; 李莎莎1; 2; 3; 骆强伟4; 徐炎1; 2; 3; 王跃进1; 2; 3; （1.西北农林科技大学园艺学院，陕西杨凌 712100；2.旱区作物逆境生物学国家重点实验室，陕西杨凌 712100；3.农业农村部西北地区园艺作物生物学与种质创制重点实验室，陕西杨凌 712100；4.新疆维吾尔自治区葡萄瓜果研究所，新疆鄯善 838200）

Author(s):: YU Saisai1; 2; 3; LI Shasha1; 2; 3; LUO Qiangwei4; XU Yan1; 2; 3; WANG Yuejin1; 2; 3; (1.College of Horticulture,Northwest Agriculture and Forestry University,Yangling,Shaanxi 712100；2.State Key Laboratory of Crop Stress Biology in Arid Areas,Yangling,Shaanxi 712100；3.Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest,Ministry of Agriculture and Rural Affairs,Yangling,Shaanxi 712100；4.Institute of Grapes and Melons on the Research in Xinjiang,Shanshan,Xinjiang 838200)

关键词:: 葡萄; 胚挽救; 无核性状; 抗寒性状; 分子标记

Keywords:: grapevine; embryo rescue; seedlessness; cold resistance; molecular marker

DOI:: 10.11937/bfyy.20212010

文献标志码:: A

摘要:: 以欧洲无核葡萄品种、抗寒性强的中国野生山葡萄后代欧山品种“左优红”和“北冰红”及其它抗寒材料为大田杂交试验材料，在胚败育前采取杂交幼果，在无菌条件下采用离体胚挽救方法获得杂种后代，并对杂种幼苗进行分子标记辅助选择，初步筛选出无核抗寒葡萄新材料。研究了杂种胚珠取样时间、亲本基因型、胚萌发培养基中添加不同种类及浓度氨基酸等对胚挽救效率的影响，以期提高无核抗寒葡萄胚挽救育种效率。结果表明：获得235个杂交植株，以“红宝石无核”和“昆香无核”为母本的杂交组合胚挽救效果较好，适宜在无核葡萄胚挽救中作母本。在幼胚离体发育阶段的MM3培养基中添加谷氨酰胺对胚挽救效果较好。无核分子标记SCF27-2000检测出32个植株携带无核特异性条带；抗寒分子标记S241-717和S238-800检测出38个植株携带抗寒特异性条带。

Abstract:: The European seedless grape varieties and the progeny of cold-hardy Chinese wild Vitis amurensis crossed with ‘Zuoyouhong’ and ‘Beibinghong’，as well as other cold resistant materials were used as female and male parents to hybrid in the field.Immature berries were gathered before embryo abortion，and hybrid progenies were obtained in vitro embryo rescue.Additionally，the molecular markers were applied to screen the new seedless and cold resistance grape from hybrid seedling.To improve the efficiency of breeding seedless and cold resistance grapes by embryo rescue，the factors of hybrid ovules sampling time，parental genotype，different kinds and concentrations of amino acids in embryo germination medium were studied,in order to improve embryo rescue efficiency of cold-resistant grapevine.The results showed that 235 hybrid plants were obtained.The hybridization combination of ‘Ruby Seedless’ and ‘Kunxiang Seedless’ as the parent had better embryo rescue efficiency and were more suitable as the parent in seedless grape embryo rescue.It was better for embryo rescue efficiency to add glutamine on MM3 at the isolated developmental stage.There were 32 hybrids carried specific bands detected by seedless molecular marker SCF27-2000，38 hybrids carried specific bands detected by cold resistant molecular markers S241-717 and S238-800.

参考文献/References:

[1]唐冬梅.无核葡萄杂交胚挽救新种质创建与技术完善[D].杨凌:西北农林科技大学,2010.[2]刘巧.利用胚挽救技术培育抗寒无核葡萄新种质[D].杨凌:西北农林科技大学,2015.[3]赵滢.山葡萄种质抗寒性评价及抗寒机制研究[D].北京:中国农业科学院,2018.[4]朱佩佩.抗寒无核葡萄杂种胚挽救及分子标记辅助选择[J].中国农业科学,2021,54(6):1218-1228.[5]LIU Q,ZHANG J,WANG Y,et al.Breeding for cold-resistant，seedless grapes from Chinese wild Vitis amurensis using embryo rescue[J].New Zealand Journal of Crop and Horticultural Science,2016,44(2):1-16.[6]RAMMING D W,EMERSHAD R L.In ovulo embryo culture of seeded and seedless Vitis vinifera L.[J].Vitis Vinifera,1982,17(11):487.[7]AKKURT M,TAHMAZ H.Recent developments in seedless grapevine breeding[J].South African Journal of Enology and Viticulture,2019,40(2):1.[8]GUO X,CHEN W,GUO Y,et al.Influencing factors of embryo rescue in seedless grape[J].Pakistan Journal of Botany,2015,47(2):669-673.[9]LI T,LI Z,YIN X,et al.Improved in vitro Vitis vinifera L.embryo development of F1 progeny of ‘Delight’× ‘Ruby seedless’ using putrescine and marker-assisted selection[J].In Vitro Cellular and Developmental Biology Plant,2018,54(3):291-301.[10]TIAN L,WANG Y.Seedless grape breeding for disease resistance by using embryo rescue[J].Vitis,2015,47(1):15-19.[11]TANG N D.Breeding of disease-resistant seedless grapes using Chinese wild Vitis spp.I.In vitro embryo rescue and plant development[J].Scientia Horticulturae,2008,117(2):136-141.[12]贾姗姗,骆强伟,李莎莎,等.葡萄胚挽救技术优化及无核和玫瑰香味新种质创制[J].中国农业科学,2020,53(16):3344-3355.[13]徐可遥,利用胚挽救技术选育香味无核葡萄新种质[D].杨凌:西北农林科技大学,2016.[14]史文静,骆强伟,王跃进.无核香味葡萄胚挽救育种研究[J].西北植物学报,2018,38(6):983-993.[15]潘学军.无核抗病葡萄胚挽救技术体系优化及新品系培育[D].杨凌:西北农林科技大学,2005.[16]王爱玲,王跃进,唐冬梅,等.提高无核葡萄胚挽救中幼胚成苗率的研究[J].中国农业科学,2010,43(20):4238-4245.[17]郭雨瑞.多效唑提高无核葡萄胚挽救育种效率研究[D].杨凌:西北农林科技大学,2018.[18]李志瑛,王跃进,骆强伟.无核葡萄胚挽救育种与杂种后代分子标记辅助选择[J].果树学报,2019,36(1):31-42.[19]赵雅楠.无核抗寒葡萄胚挽救育种与分子标记辅助选择应用[D].杨凌:西北农林科技大学,2018.[20]韩宇蕾.无核葡萄胚挽救育种与分子标记辅助选择应用[D].杨凌:西北农林科技大学,2019.[21]屈田田,张剑侠,骆强伟,等.无核葡萄抗寒抗病胚挽救育种应用研究[J].果树学报,2017,34(2):157-165.[22]ZHU P,GU B,LI P,et al.New cold-resistant，seedless grapes developed using embryo rescue and marker-assisted selection[J].Plant Cell Tissue and Organ Culture,2020,140(3):551-562.[23]贺普超.葡萄学[M].北京:中国农业出版社,1999.[24]傅雨恒.无核抗寒葡萄胚挽救研究与应用[D].杨凌:西北农林科技大学,2020.[25]王跃进,LAMIKANRA O,卢江,等.葡萄无核基因的RAPD遗传标记[J].西北农业大学学报,1996,24(5):1-10.[26]MEJIA N,HINRICHSEN P.A new highly assertive scar marker potentially useful to assist selection for seedlessness in table grape breeding[J].Acta Horticulturae,2003,603:559-564.[27]张剑侠,熊艳,王跃进,等.中国野生葡萄抗寒基因的RAPD标记及其序列分析[J].中国农学通报,2010,26(10):30-37.[28]张利,孟新法,张潞生,等.无核葡萄胚珠发育及早期离体培养的研究Ⅱ:无核葡萄胚发育的特点[J].北京农业大学学报,1991,17(4):55-59.[29]LIU S M,SYKES S R,CLINGELEFFER P R.Improved in ovulo embryo culture for stenospermocarpic grapes (Vitis vinifera L.)[J].Australian Journal of Agricultural Research,2003,54(9):869-876.[30]LI S,LIU K,YU S,et al.The process of embryo abortion of stenospermocarpic grape and it develops into plantlet in vitro using embryo rescue[J].Plant Cell Tissue and Organ Culture,2020,143(2):389-409.[31]LI S,LI Z,SHI W,et al.New disease-resistant，seedless grapes are developed using embryo rescue and molecular markers[J].3 Biotech，2020,10(1):1.[32]MOOSE S P,MUMM R H.Molecular plant breeding as the foundation for 21st Century crop improvement[J].Plant Physiology,2008,147(3):969-977.

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备注/Memo

第一作者简介：玉赛赛（1996-），女，硕士研究生，研究方向为果树育种与生物技术。E-mail：1425095025@qq.com．责任作者：王跃进（1958-），男，博士，教授，博士生导师，现主要从事果树种质资源与遗传育种等研究工作。E-mail：wangyj@nwsuaf.edu.cn.基金项目：财政部和农业农村部：国家现代农业产业技术体系资助项目。收稿日期：2021-05-11

更新日期/Last Update: 2022-02-11