QUAN Yingjie,REN Manrong,YANG Wenting,et al.Establishment of Regeneration System and Genetic Transformation System of ‘Yingjie Hongfei’ of Chrysanthemum morifolium[J].Northern Horticulture,2021,(21):72-77.[doi:10.11937/bfyy.20211861]
地被菊‘映洁红菲’再生及遗传转化体系的建立
- Title:
- Establishment of Regeneration System and Genetic Transformation System of ‘Yingjie Hongfei’ of Chrysanthemum morifolium
- Keywords:
- Chrysanthemum morifolium; regeneration system; adventitious bud; carboxybenzylpenicillin; hygromycin
- 文献标志码:
- A
- 摘要:
- 以地被菊‘映洁红菲’试管苗叶片为试材，采用组织培养法，研究了6-苄基腺嘌呤、萘乙酸激素浓度、蔗糖浓度、MS浓度、NO-3和NH+4的浓度比、羧苄青霉素浓度及潮霉素浓度对不定芽分化的影响，以期建立‘映洁红菲’高效的再生体系，为地被菊转基因体系奠定基础。结果表明：在6-BA和NAA浓度分别为 2.0、0.6 mg·L-1，蔗糖浓度为30 g·L-1，NO-3∶NH+4为30∶30，基本培养基3/4MS中，‘映洁红菲’叶片中愈伤组织诱导和不定芽分化率达到最高，平均每个叶盘分化3.22个不定芽，诱导率75.67%。在生根培养基中加入浓度为0.75 mg·L-1IBA时，根系生长最好。‘映洁红菲’遗传转化时，羧苄青霉素浓度为250 mg·L-1，潮霉素浓度为2.0 mg·L-1，适合作为选择压。
- Abstract:
- Chrysanthemum morifolium ‘Yingjie Hongfei’ were used as test material.Tissue culture method was carried out.The effects of 6-BA,NAA concentration,sucrose concentration,MS concentration,NO-3 and NH+4 concentration ratio,Carb concentration and Hyg concentration on adventitious bud differentiation of test tube plantlets of Chrysanthemum morifolium ‘Yingjie Hongfei’ were studied,in order to establish an efficient regeneration system of ‘Yingjie Hongfei’ and lay a foundation for transgenic system of Chrysanthemum morifolium.The results showed that the callus induction and adventitious bud differentiation rate of ‘Yingjie Hongfei’ leaves reached the highest in 3/4MS medium with 6-BA and NAA concentration of 2.0 mg·L-1 and 0.6 mg·L-1,sucrose concentration of 30 g·L-1,NO-3∶NH+4 concentration of 30∶30,and the average adventitious bud differentiation rate of each leaf disc was 3.22,with 75.67%.When 0.75 mg·L-1 IBA was added into rooting medium,root growth was the best.The concentration of Carb was 250 mg·L-1 and Hyg was 2.0 mg·L-1,which was suitable for selection.
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备注/Memo
第一作者简介：全英杰（1997-），女，硕士研究生，研究方向为菊花遗传育种。E-mail：1252130479@qq.com.责任作者：高日（1982-），男，博士，讲师，现主要从事菊花遗传育种等研究工作。E-mail：gaori@ybu.edu.cn.基金项目：国家自然科学基金资助项目（32060696）；延边州科技发展计划资助项目（2020FG31）；延边大学校企合作资助项目（延大科合字2020）；延边大学青年基金资助项目(延大科合字[2017]第21号)；延边大学博士科研启动基金资助项目（2018）；农业农村部景观设计重点实验室开放课题资助项目（KF201902）。收稿日期：2021-04-29