XU Dingfan,LIU Yanjun,WANG Zhaonan,et al.Establishment of Tissue Culture and Rapid Propagation System in Fraxinus velutina[J].Northern Horticulture,2021,(19):78-83.[doi:10.11937/bfyy.20211382]
绒毛白蜡组培快繁体系的建立
- Title:
- Establishment of Tissue Culture and Rapid Propagation System in Fraxinus velutina
- Keywords:
- Fraxinus velutina; stem segment; tissue culture; phytohormone; medium
- 文献标志码:
- A
- 摘要:
- 以绒毛白蜡带腋芽茎段为试材，采用组织培养法，并进行组培苗驯化移栽试验，研究了不同消毒时间对外植体、不同植物激素种类及浓度对腋芽增殖、壮苗培养和生根培养的影响，以期建立完善的绒毛白蜡组培快繁体系，为绒毛白蜡优良无性系繁育及工厂化生产提供参考依据。结果表明：最佳外植体消毒处理为 70%酒精消毒30 s+2%次氯酸钠消毒10 min，污染率仅4.44%，存活率最高为90.00%，死亡率为5.56%；最佳增殖培养基为MS+0.5 mg?L-1 BA+1.0 mg?L-1 IAA，增殖系数达5.52；最佳壮苗培养基为MS+0.5 mg?L-1 IAA，平均苗高5.83 cm；最佳生根培养基为1/2MS+0.5 mg?L-1 NAA，生根率达98.89%，平均每株根条数5.62条；生根后自然光下驯化炼苗1周，移栽到草炭∶蛭石∶珍珠岩=1∶1∶1基质中，存活率达90% 以上。
- Abstract:
- Taking the stem segment of Fraxinus velutina with axillary buds as test material,using tissue culture method,and the tissue culture seedlings were acclimated and transplanted.The effects of different disinfection time on explants,different types and concentrations of plant hormones on axillary bud proliferation,strong seedling culture and rooting culture were studied,in order to establish a perfect tissue culture rapid propagation system of Fraxinus velutina,and provide a reference for fine clone breeding and industrialized production of Fraxinus velutina.The results showed that,the optimal explant disinfection treatment was 70% alcohol disinfection for 30 seconds+2% sodium hypochlorite disinfection for 10 minutes，the pollution rate was only 4.44%，the highest survival rate was 90.00%，the mortality rate was 5.56%；the optimal proliferation medium was MS+0.5 mg?L-1 BA+1.0 mg?L-1 IAA，the proliferation coefficient was 5.52；the optimal seedling culture medium was MS+0.5 mg?L-1 IAA，the average seedling height was 5.83 cm；the optimal rooting medium was 1/2MS+0.5 mg?L-1 NAA，the rooting rate was 98.89%，the average number of roots per plant was 5.62；after rooting，the seedlings were domesticated under natural light for one week，and transplanted into peat∶vermiculite∶perlite=1∶1∶1 matrix，the survival rate was more than 90%.
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备注/Memo
第一作者简介：许丁帆（1997-），男，硕士研究生，研究方向为园艺植物组织培养与育种。E-mail:1424039435@qq.com.责任作者：刘艳军（1970-），男，硕士，高级实验师，现主要从事园艺植物组织培养和分子育种等研究工作。E-mail:liuyanjun00a@126.com.基金项目：天津市科技计划资助项目（18ZXBFNC00370）；河北省产业创新创业团队资助项目（199A2905H）；河北省创新能力提升计划资助项目（20566301D）；河北省中央引导地方科技发展资金资助项目（206Z6303G）。收稿日期：2021-03-30