[1]李孝绒,侯黔东,姜昱雯,等.‘玛瑙红’樱桃生长素响应因子基因克隆及表达分析[J].北方园艺,2021,(19):7-14.[doi:10.11937/bfyy.20210600]
　LI Xiaorong,HOU Qiandong,JIANG Yuwen,et al.Cloning and Expression Analysis of an Auxin Response Factor Gene in Cerasus pseudocerasus ‘Manaohong’[J].Northern Horticulture,2021,(19):7-14.[doi:10.11937/bfyy.20210600]

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‘玛瑙红’樱桃生长素响应因子基因克隆及表达分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2021年19 页码: 7-14 栏目: 出版日期: 2021-10-15

Title:: Cloning and Expression Analysis of an Auxin Response Factor Gene in Cerasus pseudocerasus ‘Manaohong’

作者:: 李孝绒; 侯黔东; 姜昱雯; 田田; 乔光; （贵州大学农业生物工程研究院,山地植物资源保护与种质创新省部共建教育部重点试验室，贵州贵阳 550025）

Author(s):: LI Xiaorong; HOU Qiandong; JIANG Yuwen; TIAN Tian; QIAO Guang; (Institute of Agro-bioengineering，Guizhou University/Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region，Ministry of Education，Guiyang，Guizhou 550025)

关键词:: ‘玛瑙红’樱桃; 基因克隆; 生物信息学; 表达分析

Keywords:: Cerasus pseudocerasus ‘Manaohong’; gene cloning; bioinformatics; expression analysis

DOI:: 10.11937/bfyy.20210600

文献标志码:: A

摘要:: 以‘玛瑙红’樱桃根系为试材，采用RT-PCR技术，克隆了‘玛瑙红’樱桃基因，并对其进行生物信息学分析和表达分析，以期为进一步研究‘玛瑙红’樱桃基因调控及生长发育提供参考依据。结果表明：的开放阅读框为3 498 bp，编码1 165个氨基酸，包含B3、Auxin-resp和 AUX_IAA 3个结构域，是结构域完整的ARF转录因子；系统进化树分析表明与甜樱桃同源蛋白的亲缘关系最近。荧光定量PCR结果显示，基因在不同组织中均有表达，且在根、花芽、成熟果实组织中的表达量最高；200 mg·L-1IAA处理下，在花芽中的转录水平受到诱导，6、24 h时最高；而在200 mg·L-1ABA处理下，花芽中转录水平受到抑制。推测可能是与‘玛瑙红’樱桃根系、花芽、果实生长发育相关的转录因子，参与调控‘玛瑙红’樱桃IAA、ABA激素信号转导的过程。

Abstract:: CpARF7gene was cloned by RT-PCR technology from the root tissue of Cerasus pseudocerasus  ‘Manaohong’，and its bioinformatics analysis and expression analysis were carried out，in order to provide reference for further study on the regulation of the growth and development of C.pseudocerasus  ‘Manaohong’.The results showed that the open reading frame (ORF) of contained 3 498 bp and encoded 1 165 amino acid，three domains of B3 DNA/Auxin-resp family and AUX_IAA，and was the ARF transcription factor of domain integrity.The phylogenetic tree showed the closest relationship with Prunus avium homologous protein.  The results of real-tine PCR showed that the expression level of CpARF7[STBZ] gene in root，flower buds and ripening fruits tissue was significantly higher than those in other parts.Under 200 mg·L-1 IAA treatment，the expression level of in flower bud was induced，and the highest at 6 hours/24 hours.However，the expression level of in flower buds under 200 mg·L-1 ABA treatment was inhibited.These results indicated that CpARF7may be transcription factors related to the root system，flower bud，fruit growth and development of C.pseudocerasus ‘Manaohong’，and might be involved in the regulation of IAA/ABA hormone signal transduction in C.pseudocerasus ‘Manaohong’.

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备注/Memo

第一作者简介：李孝绒（1997-），女，硕士研究生，研究方向为生物工程。E-mail:1505538395@qq.com.责任作者：乔光（1981-），男，博士，正高级实验师，现主要从事果树生理及分子生物学等研究工作。E-mail:13518504594@163.com.基金项目：黔科合基础资助项目（[2020]1Y114）；国家自然科学基金资助项目（31760552）；黔科合支撑资助项目（[2021]一般231）。收稿日期：2021-02-05

更新日期/Last Update: 2021-12-23