GAO Rui,LIANG Yan-tao,CHANG Ying.Gene Cloning and Sequence Analysis of psbA in Dryopteris fragrans(L.) Schott[J].Northern Horticulture,2014,38(19):98-102.
香鳞毛蕨psbA基因的克隆与序列分析
- Title:
- Gene Cloning and Sequence Analysis of psbA in Dryopteris fragrans(L.) Schott
- 文章编号:
- 1001-0009（2014）19-0098-05
- Keywords:
- Dryopteris fragrans(L.) Schott; psbA; RACE; gene clone
- 分类号:
- S 682.35
- 文献标志码:
- A
- 摘要:
- 以提取的香鳞毛蕨总RNA为试材，采用RACE方法得到香鳞毛蕨psbA基因全长，研究其序列的生物信息学特征并提交序列至GeneBank。结果表明：克隆的基因全长1 425 bp，开放阅读框为1 062 bp，编码353个氨基酸，与其它植物相比具有很高的同源性，基因命名为DfpsbA，GeneBank登录号为KJ728648。该研究结果可为进一步阐明香鳞毛蕨光合作用机理、基因表达与调控奠定基础，同时也为植物分类与进化提供依据。
- Abstract:
- Taking total RNA extraced from Dryopteris fragrans as material,and the RACE was employed to clone cDNA full length of psbA gene and complete bioinformatics analysis was conducted.The sequence had submitted to the GeneBank.The results showed that the full length of new cloned gene was 1 425 bp,containing an open reading frame of 1 062 bp,encoding 353 amino acids,there was high homology compared with other plant species.The new gene named DfpsbA,and its accession number was KJ728648.Successfully the result would lay a foundation for further research on photosynthesis mechanism,gene expression and regulation,as well as provide the basis for plant classification and evolution.
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备注/Memo
第一作者简介：高睿（1987-），男，硕士研究生，研究方向为资源植物学与植物分子生物学。E-mail：gaoruineau@163.com.
收稿日期：2014-04-21