ZHANG Chunlin,SHANG Xiaorui,ZHAO Xin,et al.Diversity and Structure of Rhizosphere Bacteria Community of Caragana korshinskii in the Cold and Dry Area[J].Northern Horticulture,2021,(17):97-103.[doi:10.11937/bfyy.20210375]
寒旱区柠条根际细菌群落结构与多样性研究
- Title:
- Diversity and Structure of Rhizosphere Bacteria Community of Caragana korshinskii in the Cold and Dry Area
- 文献标志码:
- A
- 摘要:
- 以柠条为试材，采用Illumina MiSeq 二代高通量测序技术对16S rDNA 基因进行测序，研究了不同地区和同一地区不同取样点的柠条根际土壤细菌微生物群落结构及多样性，以期为认识和利用柠条根际细菌微生物及对柠条抗寒抗旱性的深入研究提供参考依据。结果表明：同地区不同取样点的9个样品共检测出11个相对丰度较高的类群，相对丰度高的共有优势门是Actinobacteria(22.40%~36.41%)、Proteobacteria (18.46%~35.61%)、Acidobacteria(6.67%~25.73%)、Chloroflexi(5.04%~15.26%)和 Bacteroidetes(2.85%~6.83%)。在属水平，未知属norank_c__Subgroup_6是其共有优势属，每个样品中物种分布广泛但相对丰度较低。巴彦淖尔盟地区主要类群为Sphingomonas、Arthrobacter、Streptomyces、Nocardioides、Mycobacterium和Aeromicrobium。赤峰3个样品的细菌组成具有较大差异，主要的类群为Arthrobacter、Bacillus、Streptomyces和Sphingomonas。乌兰察布3个样品的物种组成也具有一定差异，主要类群为Arthrobacter、Bacillus和Streptomyces。柠条根际土壤细菌种类繁多，同一地区不同取样点细菌群落结构和多样性相近，不同地区间赤峰和乌兰察布样品的细菌微生物丰富性高于巴彦淖尔盟样品。
- Abstract:
- Taking Caragana korshinskii as test material,high-through put sequencing technique of Illumina MiSeq was applied to sequence 16S rDNA gene.The diversity and structure of the bacteria community of the rhizosphere soil of Caragana korshinskii of different sampling points in different regions were studied,in order to provide a reference for the further studying the microorganisms of the rhizosphere soil and the cold and drought resistance of Caragana korshinskii.The results showed that 11 high abundance phylum were detected in 9 samples from different sampling points in different regions,the common dominant phylum with high relative abundance were Actinobacteria (22.40%-36.41%),Proteobacteria (18.46%-35.61%)，Acidobacteria (6.67%-25.73%)，Chloroflexi (5.04%-15.26%) and Bacteroidetes (2.85%-6.83%).At the generic level,unknown genus norank_c__Subgroup_6 was a common dominant genus,and the distribution of bacteria in each sample was extensive but the abundance was relatively low.The high relative abundance of genera of bacteria in Bayannur were Sphingomonas,Arthrobacter,Streptomyces,Nocardioides,Mycobacterium and Aeromicrobium;significant differences existed about community structure in three sample of Chifeng,the high relative abundance of genera of bacteria were Arthrobacter,Bacillus,Streptomyces and Sphingomonas. The community structure among the three samples of Ulanqab were also differences,the high relative abundance of genera of bacteria were Arthrobacter,Bacillus and Streptomyces.There are many kinds of bacteria community in the rhizosphere soil of Caragana korshinskii,the diversity and structure of the bacteria community of different sampling points in the same area is similar,and the microbial richness of Chifeng and Ulanqab samples are higher than that of Bayannur samples.
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备注/Memo
第一作者简介：张春林（1973-），女，硕士，实验师，现主要从事微生物、植物分子生物学及种质资源挖掘选育等研究工作。E-mail：zhangcl_6688@163.com.责任作者：李龙梅（1976-），女，硕士，副教授，现主要从事观赏园艺植物栽培教学与分子生物等研究工作。E-mail：lilongmeikl@163.com.基金项目：内蒙古自治区科技创新引导资助项目(KCBJ2018012)；内蒙古自治区科技计划资助项目(2019GG007)；内蒙古自治区自然科学基金重大资助项目(2019ZD05)。收稿日期：2021-01-22