YU Song,YANG Helin,ZHU Xuetian,et al.Effects of Saline-alkali Stress on Components and Contents of Root Exudates of Phaseolus vulgaris L.[J].Northern Horticulture,2021,(17):90-96.[doi:10.11937/bfyy.20210401]
盐碱胁迫对芸豆根系分泌物组分及含量的影响
- Title:
- Effects of Saline-alkali Stress on Components and Contents of Root Exudates of Phaseolus vulgaris L.
- Keywords:
- common bean; saline-alkati stress; root exudates; pH; electrical conductivity; organic acid; amino acid; soluble sugar
- 文献标志码:
- A
- 摘要:
- 以2个芸豆品种（耐盐碱性较强的“HYD”和耐盐碱性较弱的“JW”）为试材，采用土培法，将Na2CO3、NaHCO3按指定摩尔比1∶9混合后占土壤中的质量百分比设计0%（对照，T0）和0.4%（T4）2个浓度梯度，研究了盐碱胁迫对芸豆根系分泌物组分及含量的影响，以期为进一步揭示芸豆对盐碱生境响应适应的根际生态学机制、创新芸豆耐盐碱栽培调控技术提供参考依据。结果表明：无盐碱胁迫对照“HYD”根系分泌物pH和电导率均显著（P<0.05）低于“JW”。在盐碱胁迫处理后，2个品种根系分泌物pH与电导率均有所增长。在各处理芸豆根系分泌物中检测到4种有机酸和17种游离氨基酸，且不同品种的根系分泌物组分相同。盐碱胁迫显著改变了“HYD”和“JW”根系分泌物中有机酸含量，其中分泌量最多的是乙酸。在相同处理下，“HYD”根系分泌物总氨基酸和可溶性总糖含量均高于“JW”。相比于“JW”，“HYD”释放有机酸和氨基酸能力较强，可有效改变并调节pH和电导率，进而改善盐碱根际环境。
- Abstract:
- Based on the soil culture method,two common bean varieties (the higher saltine-alkali tolerance variety ‘HYD’and the weaker saltine-alkali tolerance variety ‘JW’) were used as experimental materials.According to the mass percentage of Na2CO3 and NaHCO3 mixing at the specified molar ratio of 1∶9 in the soil,two concentration gradients 0% (control,T0) and 0.4% (T4) were designed,the effects of saline-alkali stress on the components and contents of root exucates of common bean were stuided,in order to provide a scientific theoretical basis for further revealing the rhizosphere ecological mechanism of response and adaptation of common bean to saline-alkali environment,and innovating the cultivation regulation technology for saline-alkali resistance.The results showed that under the treatment without saline-alkali stress,the pH and electrical conductivity of ‘HYD’ root exudates were significantly (P<0.05) lower than that of ‘JW’.After saline-alkali stress treatment,the pH and electrical conductivity of root exudates of two varieties increased.In this experiment,four kinds of organic acids and seventeen kinds of free amino acids were detected in the root exudates,and the components of root exudates of different varieties were the same.Saline-alkali stress significantly changed the content of organic acids in ‘HYD’ and ‘JW’ root exudates,of which acetic acid was the most secreted.Under the same treatment,the total amino acid content and total soluble sugar content in root exudates of ‘HYD’ were higher than that of ‘JW’.Compared with ‘JW’,‘HYD’ had a stronger ability to release organic acids and amino acids,which could effectively change the pH and conductivity,thus improving the saline-alkali rhizosphere environment.
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备注/Memo
第一作者简介：于崧（1984-），女，博士，副教授，现主要从事植物生理生态等研究工作。E-mail:byndys@163.com.责任作者：于立河（1960-），男，博士，教授，现主要从事植物生理生态与栽培技术等研究工作。E-mail:yulihe2002@126.com.基金项目：国家重点研发计划资助项目（2018YFD1000704）；黑龙江省博士后面上基金资助项目（LBH-Z19195）；高校学成、引进人才科研启动计划资助项目（XYB2014-02）。收稿日期：2021-01-24