ZHOU Zigeng,WANG Zibo,JIN Hualin,et al.Changes of Enzyme Activities Related to Glycolic Acid Synthesis During Fruit Development of Actinidia arguta[J].Northern Horticulture,2021,(19):22-28.[doi:10.11937/bfyy.20210191]
软枣猕猴桃果实发育过程中糖酸合成相关酶活性变化
- Title:
- Changes of Enzyme Activities Related to Glycolic Acid Synthesis During Fruit Development of Actinidia arguta
- Keywords:
- Actinidia arguta; fruit; sugar acid-related enzyme activity
- 文献标志码:
- A
- 摘要:
- 以七年生“桓优1号”和“魁绿”及五年生优系“蛟河08-03”软枣猕猴桃为试验材料，采用分光光度计法，研究了果实发育过程中糖酸合成相关酶活性变化,以期探明软枣猕猴桃果实糖酸合成机制。结果表明:果实内蔗糖的积累主要与蔗糖磷酸合成酶（sucrose phosphate，SPS）和蔗糖合成酶（sucrose synthase，SS）活性相关，但是SPS贡献最大，SS次之，而酸性转化酶（acid invertase，AI）和中性或碱性转化酶（neutral invertase，NI）对蔗糖的积累影响不大，所以总体而言果实内蔗糖含量不断提高。3个品种(系)的软枣猕猕猴均属于柠檬酸优势型果实，磷酸烯醇式丙酮酸羧化酶（phosphoenolpyruvate carbxylase,PEPC）、柠檬酸合成酶（citroyl synthetase,CS）、苹果酸脱氢酶（malic dehydrogenase,NAD-MDH）及细胞质顺乌头酸酶（cytoplasmic cisplatin enzyme,Cyt-ACO）共同促进了柠檬酸的累积，但是CS贡献最大，而线粒体顺乌头酸酶(mitochondrial aconitase,Mit-ACO)和异柠檬酸脱氢酶（isocitrate dehydrogenase,NAD-IDH）不利于柠檬酸的积累。
- Abstract:
- Taking 7-year-old ‘Huanyou No.1’ ‘Kuilyu’ varieties and 5-year-old excellent line ‘Jiaohe 08-03’ as experimental materials,the changes of enzyme activities related to sugar and acid synthesis during fruit development were studied by spectrophotometer,in order to explore the mechanism of sugar and acid synthesis in soft jujube kiwifruit.The results showed that the accumulation of sucrose in fruit was mainly related to the activities of sucrose phosphate(SPS) and sucrose synthase (SS),but SPS contributed most,followed by SS,while acid invertase(AI) and neutral invertase(NI) had little effect on the accumulation of sucrose,in general,the sucrose content in fruit increased continuously.All the soft dates of the three lines were citric acid competitive fruit,phosphoenolpyruvate carboxylase（PEPC）,citrate synthase(CS),malate dehydrogenase（NAD-MDH）and cytoplasm aconitase（Cyt-ACO） ointly promoted the accumulation of citric acid,but the largest contribution to the CS.However,the mitochondrial aconitase(Mit-ACO) and NAD-isocitrate dehydrogenase（NAD-IDH） were not conducive to citric acid accumulation.
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备注/Memo
第一作者简介：周子更（1997-），男，硕士研究生，研究方向为果树栽培生理。E-mail:2692494706@qq.com.责任作者：朴一龙（1962-），男，博士，副教授，硕士生导师，现主要从事果树栽培生理和果实采后生理等研究工作。E-mail:piaoly@ybu.edu.cn.基金项目：国家自然科学基金资助项目（31060254）。收稿日期：2021-01-04