HE Haiyong,ZHAO Dailin,TAN Qingqun,et al.Isolation,Identification and Pathogenicity of Pathogens Causing Gerbera Root Rot Disease[J].Northern Horticulture,2021,(14):87-93.[doi:10.11937/bfyy.20205507]
非洲菊根腐病病原菌的分离鉴定及致病性
- Title:
- Isolation,Identification and Pathogenicity of Pathogens Causing Gerbera Root Rot Disease
- Keywords:
- gerbera; root rot; morphological identification; molecular identification; pathogenicity test
- 文献标志码:
- A
- 摘要:
- 以非洲菊根腐病发病植株为试材，采用常规组织分离法对引起非洲菊根腐病病原菌进行分离纯化，研究了分离物对非洲菊的致病性，并通过形态学结合 rDNA-ITS和TEF-1α序列的分子系统学方法对病原菌进行鉴定，以期明确非洲菊根腐病的病原菌类型。结果表明：从典型病根上共分离获得8株菌株，分别为FZJf 1-1、FZJf 1-2、FZJf 1-3、FZJf 2-1、FZJf 2-2、FZJf 2-3、FZJf 3-1和FZJf 3-2；8株菌株均能导致非洲菊幼苗出现典型的叶片枯萎和根腐症状，其中菌株 FZJf 1-1和FZJf 1-3致病力最强，接种第3周植株开始发病，其次是菌株FZJf 2-1，接种第5周植株开始发病，其余菌株致病性较弱；根据形态学特征和分子系统学特征鉴定出6 株为尖孢镰刀菌（Fusarium oxysporum），分别是FZJf 1-1、FZJf 1-2、FZJf 1-3、FZJf 2-1、FZJf 2-3和FZJf 3-2，1 株为茄类镰刀菌(F.solani)FZJf 2-2，1 株为层出镰刀菌（F.proliferatum）FZJf 3-1，尖孢镰刀菌（F.oxysporum）为优势病原菌，茄类镰刀菌(F.solani)和层出镰刀菌（F.proliferatum）为弱势病原菌。
- Abstract:
- The disease plants of gerbera root rot were used as the experimental materials,pathogen species were isolated and purified from the gerbera root rot by the tissue isolation method,then the pathogenicity of the isolates to gerbera plants were studied,finally the pathogen species were identified by using morphological and molecular systematics method based on rDNA-ITS and TEF-1α sequences,in order to make clear the fungal pathogen species of thegerbera root rot disease.The results showed that a total of 8 pathogenic strains of FZJf 1-1,FZJf 1-2,FZJf 1-3,FZJf 2-1,FZJf 2-2,FZJf 2-3,FZJf 3-1 and FZJf 3-2 were isolated from severely rot roots;pathogenicity test results showed that all the 8 strains were pathogenic to gerbera seedlings and could cause typical leaf wilt and root rot symptoms,among which strains FZJF 1-1 and FZJF 1-3 had the strongest pathogenicity,the plants developed disease after 3 weeks inoculation,followed by strain FZJF 2-1,the plants developed disease after 5 weeks inoculation,while the other strains were less pathogenicity;6 strains of FZJf 1-1,FZJf 1-2,FZJf 1-3,FZJf 2-1,FZJf 2-3 and FZJf 3-2 were identified as Fusarium oxysporum,one strain of FZJf 2-2 was identified as F.solani and one strain of FZJf 3-1identified as F.proliferatumby morphological and molecular phylogenetic characteristics.F.oxysporum species were the dominant pathogens,and F.solani and F.proliferatum were weak pathogens.
参考文献/References:
[1]李有清,吕长平,胡春梅.非洲菊种质资源研究进展[J].湖南农业科学,2014(5):53-55.[2]李华勇,许大光,郑勤.非洲菊根腐病的发生与防治方法探讨[J].安徽农业科学,2003(4):659-659.[3]VANOS E A,BRUINS M,WOHANKA W,et al.Slow filtration:A technique to minimise the risks of spreading root-infecting pathogens in closed hydroponic systems[J].Acta Horticulturae,2001,2(559):495-502.[4]徐秉良,郁继华,王国平.非洲菊枯萎病病原菌鉴定及药剂防治[J].植物保护,2002,28(5):32-33.[5]LEBRUN D.Methods of control for Phytophthora cryptogea on Gerbera and P.cinnamomi on Chamaecyparis[J].PHM Revue Horticole,2002,442:12-16.[6]曾会才,HO H H,蒲金基,等.海南非洲菊根腐病病原的分离与鉴定[J].菌物系统,2003(2):314-318.[7]冯光恒,王继华,王丽花,等.昆明地区非洲菊疫病的PCR技术检测[J].西南农业学报,2004(S1):221-223.[8]郭成宝,张正光,王源超,等.非洲菊疫霉根腐病的快速分子诊断[J].植物病理学报,2006(2):97-101.[9]LOOKABAUGH E C,SHEW B,IVORS K.Mefenoxam sensitivity,aggressiveness,and identification of Pythium species causing root rot on floriculture crops in north carolina[J].Plant Disease,2015,99(11):1550-1558.[10]郝向阳,林觅,孙雪丽,等.福建非洲菊产区根腐病病原菌的分离与鉴定[J].福建农业学报,2018,33(4):391-395.[11]李越,李凡,刘云龙,等.云南非洲菊根腐病病原鉴定[J].华南农业大学学报,2008(3):24-28.[12]姚凤琴,林发壮,陈昌铭,等.枯草芽孢杆菌bv17对非洲菊根腐病抑菌作用及防治效果[J].福建农业科技,2019,352(12):55-59.[13]ZHAO D L,YANG X H,WU S P,et al.First report of Fusarium proliferatum causing root rot of gerbera in China[J].Journal of Plant Diseases and Protection,2020,127(2):279-282.[14]张正光,郭成宝,王源超,等.非洲菊根腐病病原的鉴定与ITS序列分析[J].植物病理学报,2005,35(5):392-396.[15]LESLIE J F,SUMMERELL B A.Fusarium laboratory manual[M].Lowa:Blackwell Pub Professional,2006.[16]LEVRONAS C,CHRETIEN,PAUL L,et al.First report of Fusarium proliferatum causing garlic clove rot in france[J].Plant Disease,2018,102(12):1-2.[17]GAO J,WANG J,YANG C,et al.Fusarium proliferatum,a new pathogen causing Codonopsis lanceolata root rot in China[J].Plant Disease,2017,101(9):1679.[18]CONG L,SUN Y,KANG J,et al.First report of root rot disease caused by Fusarium proliferatum on alfalfa in China[J].Plant Disease,2016,100(12):2526.[19]DIAZ-ARIAS M M,MUNKVOLD G P,LEANDROL F.First report of Fusarium proliferatumcausing root rot on soybean (Glycine max) in the United States[J].Plant Disease,2011,95(10):1316-1316.[20]PEREZ B A,BERRETTAM F,CARRION E,et al.First report of root rot caused by Fusarium proliferatum on blueberry in argentina[J].Plant Disease,2011,95(11):1478.
相似文献/References:
[1]沈清清,刘 芳,胡 彦.药用植物根腐病病原菌研究进展[J].北方园艺,2014,38(11):187.
SHEN Qing-qing,LIU Fang,HU Yan.Research Progress of Pathogens of Root Rot Disease on Medicinal Plants[J].Northern Horticulture,2014,38(14):187.
[2]曹利祥,苏卫国,栾业亮,等.不同药剂处理对无花果根腐病的影响[J].北方园艺,2012,36(04):140.
CAO Li-xiang,SU Wei-guo,LUAN Ye-liang,et al.Effects of Different Medicament Treatment on Ficus carica L. Root Rot[J].Northern Horticulture,2012,36(14):140.
[3]乔永旭,张永平,张红心,等.非洲菊再生体系的建立[J].北方园艺,2012,36(18):133.
QIAO Yong-xu,ZHANG Yong-ping,ZHANG Hong-xin,et al.Establishment of Regeneration System of Gerbera[J].Northern Horticulture,2012,36(14):133.
[4]李飞鹏,曹荣根,舒洋,等.四种红色系非洲菊品种特性的比较[J].北方园艺,2012,36(20):55.
LI Fei-peng,CAO Rong-gen,SHU Yang,et al.Comparison of Characteristics of Four Red Cultivars of Gerbera jamesonii[J].Northern Horticulture,2012,36(14):55.
[5]刘 旭,刘 博,吕春华,等.不同激素处理对非洲菊开花的影响[J].北方园艺,2012,36(24):83.
LIU Xu,LIU Bo,LV Chun-hua,et al.Effect of Different Hormone Treatments on Flowering of Gerbera[J].Northern Horticulture,2012,36(14):83.
[6]赵春莉,李金英.黄花乌头根腐病菌的室内药剂筛选[J].北方园艺,2012,36(19):155.
ZHAO Chun-li,LI Jin-ying.Indoor Reagent Screening of Aconitum coreanum Root Rot Disease[J].Northern Horticulture,2012,36(14):155.
[7]徐玲丽,张鸿翎,韩玲玲,等.鲜切花家用保鲜剂的配方研究[J].北方园艺,2012,36(19):165.
XU Ling-li,ZHANG Hong-ling,HAN Ling-ling,et al.Study on the Formula of the Preservation of Cut Flower Freshness[J].Northern Horticulture,2012,36(14):165.
[8]王洁琼,彭绍峰,周子发,等.非洲菊试管苗生根培养方法的优化[J].北方园艺,2012,36(13):140.
WANG Jie-qiong,PENG Shao-feng,ZHOU Zi-fa,et al.The Optimization of Gerbera Plantlets Rooting Methods[J].Northern Horticulture,2012,36(14):140.
[9]周 莹,袁孟娟,韩 军,等.中药材丹参根腐病生防菌的分离与鉴定[J].北方园艺,2015,39(01):145.[doi:10.11937/bfyy.201501039]
ZHOU Ying,YUAN Meng-juan,HAN Jun,et al.Isolation and Identification of Traditional Chinese Medicine Salvia miltiorrhiza Bunge Root Rot Biocontrol Agents[J].Northern Horticulture,2015,39(14):145.[doi:10.11937/bfyy.201501039]
[10]程亮.青海省蚕豆品种（系）抗根腐病鉴定[J].北方园艺,2015,39(06):103.[doi:10.11937/bfyy.201506028]
CHENG Liang.Resistance Identification of Broad Bean Varieties (Lines)to Root Rot in Qinghai Province[J].Northern Horticulture,2015,39(14):103.[doi:10.11937/bfyy.201506028]
备注/Memo
第一作者简介：何海永（1976-），男，硕士，副研究员，现主要从事植物真菌病害等研究工作。E-mail：405942215@qq.com.基金项目：贵州省科研机构服务企业行动计划资助项目（黔科合服企[2018]4004）；贵州省科技计划资助项目（黔科合支撑[2019]2313号，黔科合支撑[2020]4Y115号，黔科合支撑[2020]4Y114号）。收稿日期：2020-12-23