QIN Rulan,WANG Danping,MIAO Dongyu,et al.Effects of Different Drying Methods on the Chemical Constituents and Anti-oxidant Activities of Gymnospermium microrrhynchum[J].Northern Horticulture,2021,(16):123-129.[doi:10.11937/bfyy.20204973]
不同干燥方法对牡丹草化学成分及抗氧化活性的影响
- Title:
- Effects of Different Drying Methods on the Chemical Constituents and Anti-oxidant Activities of Gymnospermium microrrhynchum
- Keywords:
- Gymnospermium microrrhynchum; drying methods; ingredient; determination of content; anti-oxidant activity
- 文献标志码:
- A
- 摘要:
- 以牡丹草为试材，采用晒干、阴干、硅胶干燥等方法进行干燥处理，通过紫外可见分光光度法测定各样品中总皂苷、总黄酮及多糖含量，研究不同干燥处理方法对牡丹草化学成分的影响，并以清除DPPH自由基（DPPH?）、ABTS自由基（ABTS+?）、羟基自由基（?OH）、超氧阴离子（O?2）等多种抗氧化能力为指标，评价不同干燥方法处理后各牡丹草样品的抗氧化活性，同时结合Pearson相关性分析，探讨各样品抗氧化能力与样品中总皂苷、〖JP+2〗总黄酮及多糖含量的关系，进一步研究不同干燥处理方法对牡丹草抗氧化活性的影响，以期为牡丹草药材的炮制加工提供参考依据。结果表明：70 ℃干燥处理后的牡丹草总黄酮、总皂苷和多糖含量最高，分别为0.009 8、0.008 8、0.014 7 g?g-1，所测定的5种抗氧化指标中，70 ℃干燥处理后的牡丹草药材对O?2、ABTS+?清除能力最强，IC50分别为4.981、3.543 g?L-1；真空冷冻干燥处理后的牡丹草药材对DPPH?、?OH清除能力最强，其IC50分别为3.358、4.336 g?L-1；70 ℃干燥处理后的牡丹草药材总还原能力最强。通过Pearson相关性分析结果可知，牡丹草药材中的总皂苷及总黄酮含量与抗氧化能力存在显著相关性（P<0.05）。
- Abstract:
- Gymnospermium microrrhynchum was used as experimental material,and the Gymnospermium microrrhynchum was dried by 3 different drying methods such as sun drying,shading drying,silica drying etc.The contents of total saponins,total flavonoids and polysaccharides were determined by ultraviolet spectrophotometer.The effects of different drying methods on the contents of ingredients was investigated.The anti-oxidant activity was measured by the index including DPPH radical scavenging ability,ABTS radical scavenging ability,hydroxyl radical scavenging ability and superoxide anion free radical scavenging ability,which were implied to evaluate anti-oxidant activities of Gymnospermium microrrhynchum by different drying methods.Meanwhile,the relationships between contents of total saponins,total flavonoids,polysaccharides and anti-oxidant activities in various samples were discussed by Pearson correlation analysis.In order to provide a reference for the processing of Gymnospermium microrrhynchum.The results showed that the content of total flavonoids,total saponins and polysaccharides were 0.009 8,0.008 8 g?g-1 and 0.014 7 g?g-1 in sample dried processed at 70 ℃,which were the highest in all samples.Especially,it showed the highest scavenging activities for superoxide anion free radical and ABTS radical.The IC50 were 4.981 g?L-1 and 3.543 g?L-1.The DPPH radical scavenging ability and the hydroxyl radical scavenging ability were the highest in the sample processed by vacuum freeze drying,which were 3.358 g?L-1 and 4.336 g?L-1.The sample dried processed at 70 ℃ had the strongest total reduction ability.The relationship between the anti-oxidant activities and the total saponins,the total flavonoids were positively by Pearson correlation analysis (P<0.05).
参考文献/References:
[1]曲建博,魏娜娜,王姝麒.小檗科植物化学成分及生物活性研究概况[J].食品与药品,2013,15(4):89-91.[2]李会伟,刘培,钱大玮,等.不同干燥方法及其影响因子对玄参药材初加工过程品质形成的影响[J].中国中药杂志,2015,40(22):4417-4423.[3]吴忠旺,吴一超,王丽,等.不同干燥方法对白芍中6种化学成分的影响与评价[J].天然产物研究与开发,2016,11(14):1764-1770.[4]黄进,李娅,邱丽莎,等.不同干燥方法对白及花营养成分和抗氧化活性的影响[J].中国医院药学杂志,2017,37(19):1942-1946.[5]刘培,陈京,周冰,等.不同干燥方法及其条件对杭白芷中香豆素及挥发油化学成分的影响[J].中国中药杂志,2014,39(14):2653-2659.[6]王莹,陈圆,聂倩倩,等.女贞子中齐墩果酸的提取工艺及其抗氧化活性[J].江苏农业科学,2017,45(16):174-176.[7]高子阳,王瑞,郭夫江,等.不同种类市售海参的总皂苷含量测定[J].中国实验方剂学杂志,2014,20(15):89-92.[8]倪晓霞,王庆芬,魏浩泽,等.紫外分光光度法测定浦地灌肠液中总黄酮含量[J].中医药导报,2017,23(2):57-59.[9]张蕊,闫凤超,李佳梅,等.超声波辅助提取香菇多糖工艺研究[J].现代化农业,2017,12(17):32-34.[10]周勇,易延逵,杨晓敏,等.香菇中多糖含量测定方法的比较研究[J].食品研究与开发,2016,37(13):124-128.[11]ZHANG S L,DENG P,XU Y C,et al.Quantification and analysis of anthocyanin and flavonoids compositions,and antioxidant activities in onions with three different colors[J].Journal of Integrative Agriculture,2016,15(9):2175-2181.[12]徐洲,刘静,冯士令,等.不同干燥方法对淫羊藿多糖化学性质和抗氧化活性的影响[J].食品工业科技,2015,19(36):116-123.[13]宋梦璐,蓝孝浩,陈潇潇.三种不同干燥方式对金银花活性成分与抗氧化活性的影响[J].山东化工,2017,46(20):89-91.[14]李楠,师俊玲,王昆.14种海棠果实多酚种类及体外抗氧化活性分析[J].食品科学,2014,35(5):53-58.[15]张静,丁博,张华,等.不同干燥方法对滇重楼总皂苷含量和抗氧化活性的影响[J].中国中医药信息杂志,2016,23(7):95-97.[16]蒋增良,刘晓庆,王珍珍,等.葡萄酵素有机酸分析及其体外抗氧化性能[J].中国食品学报,2017,17(5):254-262.[17]陈玮琦,郭玉蓉,张娟,等.干燥方式对苹果幼果干酚类物质及其抗氧化性的影响[J].食品科学,2015,36(5):33-37.[18]陈燕文,王玲娜,梁从莲,等.干燥方法对金银花多糖含量的影响[J].中国现代中药,2017,19(1):88-91.[19]许绰微,张璐,赵思明,等.发芽糙米热风和微波干燥特性及品质研究[J].现代食品科技,2016,32(1):256-261.[20]朱邵晴,朱振华,郭盛,等.不同干燥方法对薄荷药材中多元功效成分的影响与评价[J].中国中药杂志,2015,24(40):4860-4867.[21]曹珍珍,周林燕,毕金峰,等.干燥方式对辣椒中活性物质含量和抗氧化能力的影响[J].中国食品学报,2017,17(2):173-181.[22]荣荣.蒙早苦荬菜活性物质的提取及抗氧化活性研究[D].呼和浩特:内蒙古农业大学,2020.
备注/Memo
第一作者简介：秦汝兰（1980-），女，博士，副教授，现主要从事中药化学成分与药效学等研究工作。E-mail:qrl_1949@163.com.责任作者：吕重宁（1986-），男，博士，副教授，现主要从事中药新药研究和中药药效物质基础等研究工作。E-mail:lcnmi@outlook.com.基金项目：辽宁省教育厅高校基本科研资助项目（2017LQN09）。收稿日期：2020-11-24