[1]罗跃,刘旭东,韩磊,等.贵州省辣椒炭疽病病原鉴定及协同增效药剂筛选[J].北方园艺,2021,(14):21-30.[doi:10.11937/bfyy.20204677]
　LUO Yue,LIU Xudong,HAN Lei,et al.Identification of the Pathogen of Capsicum Anthracnose and Screening of Synergistic Agents in Guizhou Province[J].Northern Horticulture,2021,(14):21-30.[doi:10.11937/bfyy.20204677]
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贵州省辣椒炭疽病病原鉴定及协同增效药剂筛选

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2021年14 页码: 21-30 栏目: 出版日期: 2021-07-30
Title:
Identification of the Pathogen of Capsicum Anthracnose and Screening of Synergistic Agents in Guizhou Province
作者:
罗跃1; 2; 3; 刘旭东1; 2; 3; 韩磊1; 2; 3; 姚小龙1; 2; 3; 耿广东3; 吴小毛1; 2; 3
（1.贵州大学 作物保护研究所，贵州 贵阳 550025；2.贵州省山地农业病虫害重点实验室，贵州 贵阳 550025；3.贵州大学 农学院，贵州 贵阳 550025）
Author(s):
LUO Yue1; 2; 3; LIU Xudong1; 2; 3; HAN Lei1; 2; 3; YAO Xiaolong1; 2; 3; GENG Guangdong3; WU Xiaomao1; 2; 3
(1.Institute of Crop Protection,Guizhou University,Guiyang,Guizhou 550025;2.The Provincial Key Laboratory for Agricultural Pest Management in Mountainous Region,Guiyang,Guizhou 550025；3.Agricultural College,Guizhou University,Guiyang,Guizhou 550025)
关键词:
辣椒; 炭疽病; 鉴定; 防治药剂; 协同增效
Keywords:
pepper; anthracnose; identification; control agent; synergy
DOI:
10.11937/bfyy.20204677
文献标志码:
A
摘要:
以采自贵州省贵阳市和遵义市的辣椒炭疽病病样为试材，采用组织分离法分离病原菌，依照柯赫氏法则确定病原菌的致病性，根据菌株形态特征及rDNA-ITS序列分析鉴定，研究引起贵州省辣椒炭疽病的病原；采用菌丝生长抑制法，研究了致病菌株对甲基硫菌灵等11种原药和10%苯醚甲环唑等6种成品药的敏感性，从而在此基础上选择不同作用机理的药剂组合复配，测定抑制活性。结果表明：结合形态学及分子生物学确定其为辣椒炭疽病菌，分别为尖孢炭疽菌（Colletotrichum acutatum）、斯高维尔炭疽菌（Colletotrichum scovillei）和疑似新种（Colletotrichum sp.）；经敏感性测定，所选药剂对菌株均有一定程度的抑制作用；咪鲜胺、双苯菌胺、唑胺菌酯、戊唑醇等4种原药对3株辣椒炭疽病菌药效比较稳定，抑制效果较好；辣椒炭疽病菌对22.50%啶氧菌酯和10%苯醚甲环唑2种成品药剂的敏感性较高，EC50值分别为1.60 mg?L-1和2.12 mg?L-1；戊唑醇与吡唑醚菌酯3∶1和1∶1复配时，SR分别为1.745 3和2.061 1，1∶1时增效效果最好。啶氧菌酯与咪鲜胺1∶3、1∶1和1∶5复配时，SR分别为1.723 4，1.661 1和1.658 5，1∶3增效效果最好。综上所述，引起贵州省辣椒炭疽病的病原菌有GY-1尖孢炭疽菌（C.acutatum）；ZY-1斯高维尔炭疽菌（C.scovillei）；ZY-2疑似新种(Colletotrichum sp.)。
Abstract:
Capsicum anthracnose samples collected from Guiyang and Zunyi in Guizhou Province were used to isolate the pathogen by tissue isolation method.The pathogenicity of the pathogen was determined according to Koch′s law.The pathogen causing pepper anthracnose in Guizhou Province was studied according to the morphological characteristics and rDNA-ITS sequence analysis;mycelial growth inhibition method was used to study the sensitivity of pathogenic strains to 11 original drugs such as thiophanate methyl and 6 finished products such as 10% difenoconazole.On this basis,different combinations of drugs with different mechanisms were selected to determine the inhibitory activity.The results showed that,combining morphology and molecular biology,it was determined that it was the pathogen of capsicum anthracnose,which were C.acutatum,C.scovillei and suspected new species (Colletotrichum sp.).The sensitivity test results showed that the selected funguscides had a certain degree of inhibitory effect on the strains.The 4 original medicines,including prochloraz,dibenzamide,pyraclostrobin,and tebuconazole,had relatively stable efficacy against 3 strains of capsicum anthracnose.The inhibitory effect was better,capsicum anthracnose was more sensitive to 2 funguscides,22.5% picoxystrobin and 10% difenoconazole,with EC50 values of 1.60 mg?L-1 and 2.12 mg?L-1,respectively.When alcohol and pyraclostrobin were compounded at 3∶1 and 1∶1,the SR was 1.745 3 and 2.061 1,respectively,and the synergistic effect was the best at 1∶1.When picoxystrobin and prochloraz were compounded at 1∶3,1∶1 and 1∶5,the SR was 1.723 4,1.661 1 and 1.658 5 respectively,and the synergistic effect of 1∶3 was the best.In conclusion,the pathogens causing capsicum anthracnose in Guizhou Province are GY-1 (C.acutatum);ZY-1 (C.scovillei);ZY-2 suspected new species (Colletotrichum sp.).

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备注/Memo

第一作者简介：罗跃（1996-），男，硕士研究生，研究方向为农药科学应用与风险评估、农药残留与环境行为、农产品质量与安全评价。E-mail：1987957869@qq.com.责任作者：吴小毛（1978-），男，博士，教授，现主要从事农药科学应用与风险评估、农药残留与环境行为、农产品质量与安全评价等研究工作。E-mail：wuxm827@126.com.基金项目：贵州省科技成果应用及产业化计划资助项目（黔科合成果[2020]1Z002号，[2021]一般059）；贵州省教育厅重点资助项目（黔教合KY字[2019]010）。收稿日期：2020-11-06

更新日期/Last Update: 2021-10-27