[1]吴琼,吴文龙,李维林,等.NAA处理对黑莓果实品质及细胞壁酶活性的影响[J].北方园艺,2021,(10):15-21.[doi:10.11937/bfyy.20203701]
　WU Qiong,WU Wenlong,LI Weilin,et al.Effects of NAA Treatment on Fruit Quality and Cell Wall Degrading Enzyme Activities of Blackberry Fruits[J].Northern Horticulture,2021,(10):15-21.[doi:10.11937/bfyy.20203701]
点击复制

NAA处理对黑莓果实品质及细胞壁酶活性的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2021年10 页码: 15-21 栏目: 出版日期: 2021-05-30
Title:
Effects of NAA Treatment on Fruit Quality and Cell Wall Degrading Enzyme Activities of Blackberry Fruits
作者:
吴琼1; 吴文龙1; 李维林2; 张春红1
（1.江苏省中国科学院 植物研究所，江苏 南京 210014；2.南京林业大学 林学院，江苏 南京 210037）
Author(s):
WU Qiong1; WU Wenlong1; LI Weilin2; ZHANG Chunhong1
(1.Institute of Botany，Jiangsu Province and Chinese Academy of Sciences，Nanjing，Jiangsu 210014；2.Academy of Forestry，Nanjing Forestry University，Nanjing，Jiangsu 210037)
关键词:
黑莓; 萘乙酸; 果实品质; 细胞壁降解酶
Keywords:
blackberry (Rubus spp.); NAA; fruit quality; cell wall degrading enzyme
DOI:
10.11937/bfyy.20203701
文献标志码:
A
摘要:
以国内黑莓主栽品种‘三冠王’为试材，采用在青果转色期喷施400 mg?L-1萘乙酸（NAA）的方法，研究了NAA对黑莓果实转色后果实性状、营养品质性状和细胞壁降解酶活性的影响，以期为探究黑莓果实硬度性状改良提供参考依据。结果表明：‘三冠王’果实在青果转色后12 d开始着色成熟，而NAA处理则为处理后15 d开始着色成熟，至18 d时均可见完全成熟的果实。与对照相比，NAA处理果实成熟后期横、纵径明显增大，单果质量显著增加。与此同时，NAA处理后果实在成熟进程中可溶性固形物和花色苷含量降低，而果实成熟时变化并不显著，至果实成熟时总多酚含量明显降低。在果实成熟后期，NAA处理果实纤维素酶和多聚半乳糖醛酸酶活性显著降低，果实β-D-木聚糖苷酶活性在成熟进程中与对照果实变化趋势类似。NAA处理后果实NAA含量在转色至成熟进程中呈逐步上升趋势。因而，NAA的积累可促进黑莓发育成熟进程中果实膨大，但对果实营养成分积累有一定减缓效应，而主要细胞壁降解酶的活性降低则利于果实硬度提高。
Abstract:
The main domestic blackberry variety ‘Triple Crown’ were used as experimental materials.The factor was treated with 400 mg?L-1 NAA at veraison of the green fruits to investigate the effects of synthetic auxin NAA on fruit characters，nutritional quality and cell wall degrading enzyme activities.In order to provide a reference basis for exploring the improvement of blackberry fruit firmness.The results showed that fruits of ‘Triple Crown’ turned to fruit coloring at 12 days after green fruit stage，whereas they turned to fruit coloring at 15 days after NAA treatment.Both fully mature fruits could be seen at 18 days after green fruit stage.As compared with the control fruits，the horizontal and vertical diameter and the single fruit weight were significantly increased after NAA treatment.In the meantime，the contents of soluble solids and anthocyanins decreased during fruit ripening，but the changes were not significant when the fruit was ripe.By comparison，the total polyphenol content in the fruits was significantly decreased after NAA treatment.The activities of cellulase and polygalacturonase in fruits of NAA treatment decreased significantly at the late stage of fruit ripening，while the change trend of β-D-xylosidase activity was similar to that of control fruits during the whole ripening process.During the process of fruit color changing and ripening，the content of NAA in the treatment group increased gradually.Therefore，the accumulation of NAA could promote the expansion of blackberry mature fruits during the fruit development and ripening stages，but it might slow down the accumulation of some nutrients in the fruits.Nevertheless，the degradation of main cell wall degrading enzyme activities in fruits of NAA treatment group may be beneficial to improve fruit firmness.

参考文献/References:

[1]SELLAPPAN S,AKOH C C,KREWER G.Phenolic compounds and antioxidant capacity of georgia-grown blueberries and blackberries[J].Journal of Agricultural and Food Chemistry,2002,50(8):2432-2438.[2]JUNQUEIRA-GONCALVES M P,ALARCN E,NIRANJAN K.The efficacy of potassium sorbate-coated packaging to control postharvest gray mold in raspberries，blackberries and blueberries[J].Postharvest Biology and Technology,2016,111:205-208.[3]CLARK J R,FINN C E.New trends in blackberry breeding[C].Pucn:ISHS Acta Horticulturae 777,IX International Rubus and Ribes Symposium,2005.[4]GUAN W B,XU P J,WANG K,et al.Determination and study on dissipation of 1-naphthylacetic acid in garlic and soil using high per formance liquid chromatography-tandem mass spectrometry[J].Food and Chemical Toxicology,2011,49(2011):2869-2874.[5]肖艳,黄建昌,李宏彬.萘乙酸和硼对草莓果实生长发育的影响[J].中国南方果树,1998(4):35-36.[6]MILI〖KG-*3〗C〖DD(-*2/3〗′〖DD)〗 B,TARLANOVI〖KG-*3〗C〖DD(-*2/3〗′〖DD)〗 J,KESEROVI〖KG-*3〗C〖DD(-*2/3〗′〖DD)〗 Z,et al.Bioregulators can improve fruit size，yield and plant growth of northern highbush blueberry (Vaccinium corymbosum L.)[J].Scientia Horticulturae,2018,235:214-220.[7]DAMINATO M,GUZZO F,CASADORO G.A SHATTERPROOF-like gene controls ripening in non-climacteric strawberries，and auxin and abscisic acid antagonistically affect its expression[J].Journal of Experimental Botany,2013,64(12):3775-3786.[8]赵权,高静.NAA和6-BA对山葡萄果实着色及相关品质的影响[J].安徽农业科学,2011,29(30):18443-18445.[9]FIGUEROA C R,PIMENTEL P,DOTTO M C,et al.Expression of five expansin genes during softening of Fragaria chiloensis fruit effect of auxin treatment[J].Postharvest Biology and Technology,2009,53:51-57.[10]FIGUEROA C R,OPAZO M C,VERA P,et al.Effect of postharvest treatment of calcium and auxin on cell wall composition and expression of cell wall-modifying genes in the Chilean strawberry (Fragaria chiloensis) fruit[J].Food Chemistry,2011,132(2012):2014-2022.[11]付润山,姜妮娜,饶景萍,等.赤霉素和萘乙酸对柿果实采后成熟软化生理指标的影响[J].西北植物学报,2010,30(6):1204-1208.[12]杨德兴,戴京晶,庞向宇,等.猕猴桃衰老过程中PG、果胶质和细胞壁超微结构的变化[J].园艺学报,1993(4):341-345.[13]BUSTAMANTE C A,CIVELLO P M,MARTNEZ G A.Cloning of the promoter region of b-xylosidase (FaXyl1) gene and effect of plant growth regulators on the expression of FaXyl1 in strawberry fruit[J].Plant Science,2009,177:49-56.[14]赵慧芳,王小敏,闾连飞,等.黑莓果实中花色苷的提取和测定方法研究[J].食品工业科技,2008(5):176-179.[15]赵慧芳,吴文龙,马丽,等.基于抗氧化活性分析的蓝莓多酚提取工艺[J].食品工业科技,2015,36(5):251-254,266.[16]陶可全,刘海军.寒地小浆果产业情况报告[J].北方园艺,2016(10):175-177.[17]李亚东,唐雪东,袁菲,等.我国小浆果生产现状、问题和发展趋势[J].东北农业大学学报,2011,42(1):1-10.[18]吴文龙,闾连飞,李维林,等.不同品种黑莓在南京地区的结实表现[J].林业科技开发,2008,22(4):24-29.[19]朱敏,邓穗生,何书强,等.NAA和爱多收对海南‘贵妃’芒产量和果实品质的影响[J].中国农学通报,2015,31(1):116-121.[20]李建国,黄旭明,黄辉白.NAA增大荔枝果实及原因分析[J].华南农业大学学报(自然科学版),2004,25(2):10-12.[21]马玮,史玉滋,段颖,等.南瓜果实淀粉和可溶性固形物研究进展[J].中国瓜菜,2018,31(11):1-5.[22]张文,韩昕炜,张静,等.萘乙酸对番茄品质的影响及其在番茄中的残留检测[J].辽宁化工,2015,44(4):375-378,381.[23]赵慧芳,吴文龙,闾连飞,等.黑莓果实发育过程中多酚类物质的变化规律[J].吉林农业大学学报,2013,35(4):457-462.[24]BTTCHER C,HARVEY K,FORDE C G,et al.Auxin treatment of pre-veraison grape (Vitis vinifera L.) berries both delays ripening and increases the synchronicity of sugar accumulation[J].Australian Journal of Grape and Wine Research,2010,17(1):1-8.[25]熊喜红,徐晓云,张强,等.湖北和云南地区蓝莓果实加工特性研究[J].北方园艺,2020(2):23-31.[26]KEEGSTRA K.Plant cell walls[J].Plant Physiology,2010,154(2):483-486.[27]BRUMMELL D A,HARPSTER M H.Cell wall metabolism in fruit softening and quality and its manipulation in transgenic plants[J].Plant Cell Walls,2001,47:311-340.[28]LAPORNIK B,PROSEK M,WONDRA A G.Comparison of extracts prepared from plant by-products using different solvents and extraction time[J].J Food Eng,2005,71(2):214-222.[29]ZHANG C H,XIONG Z H,YANG H Y,et al.Changes in pericarp morphology，physiology and cell wall composition account for flesh firmness during the ripening of blackberry (Rubus spp.) fruit[J].Scientia Horticulturae,2019,250:59-68.[30]汤勇,蔡俊.β-木糖苷酶的研究进展[J].中国酿造,2018,37(10):14-19.[31]BUSTAMANTE C A,CIVELLO P M,MARTNEZ G A.Cloning of the promoter region of β-xylosidase gene and effect of plant growth regulators on the expression of FaXyl1 in strawberry fruit[J].Plant Science,2009,177:49-56.

相似文献/References:

[1]张春红,吴文龙,王小敏,等.黑莓“宝森”及其芽变品种“宁植1号”皮刺的比较鉴定[J].北方园艺,2013,37(08):26.
　ZHANG Chun-hong,WU Wen-long,WANG Xiao-min,et al.Comparative Observation of Prickle Between Blackberry cv. ‘Boysenberry’ and Its Bud Mutation cv. ‘Ningzhi 1’[J].Northern Horticulture,2013,37(10):26.
[2]张玉平,姜明,鲁绍伟,等.黑莓亲缘关系的AFLP分析[J].北方园艺,2013,37(21):116.
　ZHANG Yu-ping,JIANG Ming,LU Shao-wei,et al.Genetic Relationship Analysis of Blackberries by AFLP[J].Northern Horticulture,2013,37(10):116.
[3]王增池,孔德平,曹永胜.萘乙酸对水培龟背竹根系的影响[J].北方园艺,2012,36(20):57.
　WANG Zeng-chi,KONG De-ping,CAO Yong-sheng.Effect of NAA on Hydroponics Monstereo Root[J].Northern Horticulture,2012,36(10):57.
[4]孙睿,邵红,李秀霞,等.萘乙酸对一串红种子萌发及幼苗生长的影响[J].北方园艺,2012,36(09):64.
　SUN Rui,SHAO Hong,LI Xiu-xia,et al.Effects of Naphthylacetate on Seed Germination and Seedling Growth of Salvia spleredens[J].Northern Horticulture,2012,36(10):64.
[5]杨海燕,张春红,吴文龙,等.黑莓果实发育过程中抗氧化系统变化规律研究[J].北方园艺,2014,38(16):13.
　YANG Hai-yan,ZHANG Chun-hong,WU Wen-long,et al.Changes on Antioxidant System in Fruit of Rubus spp.cv ‘Arapaho’ During the Development and Ripening Stage[J].Northern Horticulture,2014,38(10):13.
[6]王云生.我国野生越橘资源多样性及其最新研究动态[J].北方园艺,2016,40(04):191.[doi:10.11937/bfyy.201604046]
　WANG Yunsheng.Diversity of Wild Vaccinium Resources in China and Its Latest Research Dynamics[J].Northern Horticulture,2016,40(10):191.[doi:10.11937/bfyy.201604046]
[7]李 　 倩,刘 延 吉.黑莓果实次生物质及花色苷组分的研究[J].北方园艺,2011,35(16):0.[doi:10.11937/bfyy.2011016009]
　.Ａ ｎ ａ ｌ ｙ ｓ ｉ ｓ 　ｏ ｆ 　Ｓ ｅ ｃ ｏ ｎ ｄ ａ ｒ ｙ Ｍ ｅ ｔ ａ ｂ ｏ ｌ ｉ ｔ ｅ ｓ 　Ｂ ｌ ａ ｃ ｋ ｂ ｅ ｒ ｒ ｙ Ｆ ｒ ｕ ｉ ｔ 　Ｃ ｏ ｍ ｐ ｏ ｓ ｉ ｔ ｉ ｏ ｎ 　ｏ ｆ 　Ａ ｎ ｔ ｈ ｏ ｃ ｙ ａ ｎ ｉ ｎ[J].Northern Horticulture,2011,35(10):0.[doi:10.11937/bfyy.2011016009]
[8]黄 诚 梅,江 　 文,韦 昌 联,等.萘乙酸与多效唑对茉莉成花及新梢等生理指标的影响[J].北方园艺,2009,33(12):0.[doi:10.11937/bfyy.2009012057]
　HUANG Cheng -mei,JIANG Wen,WEI Chang -lian,et al.Effects of NAA and PP 333 on Floral Bud Formation and Physiological Indices of Jasminumsambac L.[J].Northern Horticulture,2009,33(10):0.[doi:10.11937/bfyy.2009012057]
[9]马生健,莫颜娟,曾富华.不同生长调节剂对水仙生长和开花的影响[J].北方园艺,2011,35(08):0.[doi:10.11937/bfyy.201108039]
　[J].Northern Horticulture,2011,35(10):0.[doi:10.11937/bfyy.201108039]
[10]张 素 芳,胡 书 红,杨 春 梅.一 品 红 枝 条 扦 插 生 根 研 究[J].北方园艺,2007,31(08):0.[doi:10.11937/bfyy.200708075]
　ZHANG Su - fang,HU Shu - hong,YANG Chun - mei.Guttage Experimentation of Common Poinsettia T ress[J].Northern Horticulture,2007,31(10):0.[doi:10.11937/bfyy.200708075]

备注/Memo

第一作者简介：吴琼（1996-），女，硕士研究生，研究方向为小浆果种质资源鉴定。E-mail：wqnj77@163.com.责任作者：张春红（1979-），女，博士，副研究员，现主要从事黑莓种质资源鉴定利用等研究工作。E-mail：chzhang@cnbg.net.基金项目：江苏省农业科技自主创新资金资助项目（CX(18)2017）；国家自然科学基金资助项目（31600553）；江苏省农业三新工程资助项目（SXGC[2017]309）。收稿日期：2020-08-28

更新日期/Last Update: 2021-08-09