MENG Jiangling,HE De,LI Cuixin.Tolerance and Enrichment Characteristics of Cordyceps militaris to Metal Zinc[J].Northern Horticulture,2021,(06):123-128.[doi:10.11937/bfyy.20202187]
蛹虫草对金属锌的耐受性与富集特征
- Title:
- Tolerance and Enrichment Characteristics of Cordyceps militaris to Metal Zinc
- Keywords:
- Cordyceps militaris; zinc; mycelia; fruiting body
- 文献标志码:
- A
- 摘要:
- 以蛹虫草为试材，通过在培养基中添加不同浓度的锌，利用罗丹明B分光光度法测定菌丝体和子实体锌含量，蒽酮-硫酸法和DNS法测定子实体多糖和还原糖含量，探究了锌对蛹虫草菌丝体、子实体生长和生理活性的影响，以期为富锌蛹虫草培育提供参考依据。结果表明：锌对蛹虫草菌丝体、子实体均有一定的影响，适量浓度促进生长，过高则抑制生长，最适合蛹虫草生长的培养基锌浓度为678 mg?kg-1基，该浓度下蛹虫草子实体生长良好无退化现象，干质量出现最大值为3.56 g，多糖含量为7.32％，锌富集率达6.45%。
- Abstract:
- Taking Cordyceps militaris as test material,different concentrations of zinc were added to the culture medium to analyze the effects of zinc on the growth and biological activity of mycelia and fruiting bodies.Zinc content of mycelium and fruiting body was determined by Rhodamine B spectrophotometry,the polysaccharide content of stator substance was measured by anthrone-sulfuric acid,and the reducing sugar content of stator substance was determined by DNS method,in order to provide reference for the cultivation of zinc-rich Cordyceps militaris.The results showed that zinc had a certain effect on the mycelium and fruiting body,and the growth was inhibited when the concentration was too high.The most suitable medium for the growth of Cordyceps militaris was 678 mg?kg-1基基.At this concentration,zinc had a great effect on the fruiting body,with maximum dry weight of 3.56 g,the polysaccharide content of 7.32%,zinc enrichment ratio of 6.45%.
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备注/Memo
第一作者简介：孟江玲(1995-)，女，硕士研究生，研究方向为资源微生物开发与利用。E-mail:635553131@qq.com.责任作者：何德(1970-)，男，博士，副教授，硕士生导师，现主要从事食药用真菌的开发与利用等研究工作。E-mail:1419615009@qq.com.基金项目：云南省优势特色学科生物学一级学科建设资助项目（50097505）；云南省高校林木生物技术重点实验室资助项目（2018KF19）。收稿日期：2020-05-28