YANG Zhenfu,CHEN Hui,GUO Xiang,et al.Identification and Biological Characteristics of Two Strains From Wild Agrocybe[J].Northern Horticulture,2021,(06):116-122.[doi:10.11937/bfyy.20202351]
两株田头菇属野生菌株的鉴定及生物学特性
- Title:
- Identification and Biological Characteristics of Two Strains From Wild Agrocybe
- Keywords:
- Agrocybe; isolation; identification; biological characteristic
- 文献标志码:
- A
- 摘要:
- 以采自云南的2株田头菇属野生菌株JZ061和JZ527为试材，采用组织分离法进行菌株分离，按照形态和分子生物学鉴定确定2株菌株的分类学地位，并对菌丝体的生物学特性进行研究，以期为今后进一步开发利用野生田头菇属种质资源提供参考依据。结果表明：菌株JZ061为茶树菇（Agrocybe aegerita），最适碳源为麦芽糖、乳糖和蔗糖，最适氮源为酵母粉和黄豆粉，最适pH为6.0，适宜温度为25~30 ℃；菌株JZ527为杨柳田头菇（Agrocybe salicacola），最适碳源为麦芽糖，最适氮源为酵母粉和黄豆粉，最适pH为8.0~9.0，适宜温度为20~25 ℃。
- Abstract:
- Two wild strains JZ061 and JZ527 of Agrocybe collected from Yunnan Province were used as experimental materials,and the strains were isolated by the tissue isolation.Taxonomic study of the two strains were based on morphological and phylogenetical analyses,and their biological characteristics of mycelia were studied,in order to provide reference for the application of the wild Agrocybe source.The results showed that the strain JZ061 was confirmed to be Agrocybe aegerita,and the optimum carbon source was maltose,lactose and sucrose,the optimum nitrogen source was yeast and soybean powder,the optimum pH was 6.0,and the optimum temperature range was 25-30 ℃基;the strain JZ527 was confirmed to be Agrocybe salicacola,and the optimum carbon source was maltose,the optimum nitrogen source was yeast powder and soybean powder，and the optimum pH was 8.0-9.0.Suitable temperature range was 20-25 ℃.
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备注/Memo
第一作者简介：杨珍福（1988-），女，硕士，助理研究员，现主要从事食用菌菌种选育与栽培技术等研究工作。E-mail基:ynjcyzf152@126.com.责任作者：孙达锋（1977-），男，硕士，研究员，现主要从事食用菌资源开发利用等研究工作。E-mail:sdafeng@163.com.基金项目：农业农村部食用菌加工重点实验室开放课题资助项目（RD08）；云南省科技厅基础研究计划资助项目（2018FD171）。收稿日期：2020-06-08