HAN Duohong,WANG Enjun,ZHANG Yong,et al.Effects of Exogenous γ-aminobutyric Acid （GABA） on Reactive Oxygen Species and Antioxidant System of Isatis indigotica Fort.Seedlings Under Salt Stress[J].Northern Horticulture,2021,(06):111-116.[doi:10.11937/bfyy.20201151]
外源γ-氨基丁酸对盐胁迫下菘蓝幼苗活性氧和抗氧化系统的影响
- Title:
- Effects of Exogenous γ-aminobutyric Acid （GABA） on Reactive Oxygen Species and Antioxidant System of Isatis indigotica Fort.Seedlings Under Salt Stress
- Keywords:
- Isatis indigotica Fort.; γ-aminobutyric acid(GABA); salt stress; reactive oxygen species; antioxidant enzyme
- 文献标志码:
- A
- 摘要:
- 以菘蓝为试材，对100 mmol?L-1基 NaCl溶液胁迫后的幼苗，叶面喷施浓度为0、3、6、9、12 mmol?L-1基的外源γ-氨基丁酸，测定幼苗中活性氧（ROS）含量和抗氧化酶活性，以期为增强菘蓝幼苗的耐盐性提供参考依据。结果表明：NaCl胁迫下幼苗中超氧阴离子（O?[TX-*4]基2）产生速率、过氧化氢（H2O2）含量均大幅升高，较对照增加120.73%、113.09%；叶面喷施9 mmol?L-1基的外源γ-氨基丁酸后，超氧阴离子（O?[TX-*4]基2）产生速率、过氧化氢（H2O2）含量较NaCl胁迫降低30.69%、41.10%，超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）、抗坏血酸过氧化物酶（APX）活性升高83.47%、42.38%、34.80%和76.91%；NaCl胁迫下，外源γ-氨基丁酸浓度与超氧阴离子（O?[TX-*4]基2）产生速率、过氧化氢（H2O2）含量呈负相关，与超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）、抗坏血酸过氧化物酶（APX）活性呈正相关。综合分析，外源γ-氨基丁酸缓解NaCl胁迫效果为9 mmol?L-1基＞6 mmol?L-1基基＞12 mmol?L-1基＞3 mmol?L-1基。
- Abstract:
- Isatis indigotica Fort. was used as experimental materials，the seedlings were treated with 100 mmol?L-1基 基NaCl solution，the concentrations of 0,3,6,9,12 mmol?L-1基 exogenous γ-aminobutyric acid were sprayed on the leaves,the content of reactive oxygen species(ROS)and antioxidant enzyme activities were determined in the seedlings in order to provide reference for enhancing salt tolerance of Isatis indigotica Fort..The results showed that the O?[TX-*4]基2 productive rate and H2O2 content in seedlings under NaCl stress were significantly increased,increasing by 120.73% and 113.09% compared with the control group.Spraying on the leaf 9 mmol?L-1基 of exogenous γ-aminobutyric acid,the O?[TX-*4]基2 productive rate and H2O2 content in NaCl stress reduced by 30.69%,41.10%,superoxide dismutase (SOD),peroxidase(POD),catalase(CAT) and ascorbic acid peroxidase(APX) activity increased 83.47%,42.38%,34.80% and 76.91%.Under NaCl stress,exogenous γ-aminobutyric基 acid concentration was negatively correlated with O?[TX-*4]基2 productive rate and H2O2 content,exogenous γ-aminobutyric基 acid concentration was positively correlated with the activities of superoxide dismutase(SOD),peroxidase (POD),catalase(CAT) and ascorbic acid peroxidase(APX)activity.According to the comprehensive analysis,the effects of exogenous γ-aminobutyric acid on relieving NaCl stress was 9 mmol?L-1基>6 mmol?L-1基>12 mmol?L-1基>3 mmol?L-1基.
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备注/Memo
第一作者简介：韩多红（1977-），男，本科，副教授，现主要从事中药材栽培及药用植物资源利用等研究工作。E-mail:handuohong@163.com.基金项目：甘肃省河西走廊特色资源利用省级重点实验室面上资助项目（XZ1405）；甘肃省高校产业支撑引导资助项目(2019C-01)；全国第四次中药资源普查甘肃省甘州区中药资源普查资助项目(GSZYPC201815)；甘肃省省级大学生创新创业训练计划资助项目（201610740002）。收稿日期：2020-03-24