JIANG Yuntian,QU Guangnan,GUO Yi,et al.Effects of Exogenous SA and GB on Seed Germination of Impatiens balsamena Under NaCl Stress[J].Northern Horticulture,2021,(05):77-83.[doi:10.11937/bfyy.20201121]
外源水杨酸和甜菜碱对NaCl胁迫下茶花凤仙种子萌发的影响
- Title:
- Effects of Exogenous SA and GB on Seed Germination of Impatiens balsamena Under NaCl Stress
- 文献标志码:
- A
- 摘要:
- 以茶花凤仙(Impatiens balsamena)种子为试材，采用双层滤纸法，研究了100 mmol?L-1NaCl胁迫条件下，外源SA(0.5、1.0、1.5、2.0 mmol?L-1)和GB(0.4、0.8、1.2、1.6 mmol?L-1)浸种预处理对茶花凤仙种子萌发及幼苗生长的影响，以期明确茶花凤仙种子萌发期2种外源物质对NaCl胁迫的缓解效应，降低NaCl胁迫对茶花凤仙的伤害。结果表明：NaCl胁迫显著抑制了茶花凤仙种子的萌发，发芽率、发芽势、发芽指数、活力指数、胚芽长、胚根长和苗鲜质量均显著下降，平均发芽时间显著延长；1.5 mmol?L-1SA和0.8、1.2 mmol?L-1GB对NaCl胁迫下茶花凤仙种子萌发和幼苗生长具有明显促进作用，而SA和GB浓度过高或过低则对NaCl胁迫的缓解效应下降，甚至加重盐害。利用隶属函数综合评价得出，100 mmol?L-1NaCl胁迫下，1.5 mmol?L-1SA和0.8 mmol?L-1GB对茶花凤仙种子萌发及幼苗生长的综合调控效果较好，且0.8 mmol?L-1GB对NaCl胁迫的缓解效果优于1.5 mmol?L-1SA。
- Abstract:
- To define the role of exogenous salicylic acid and exogenous glycine betaine in alleviating the damage of NaCl stress on Impatiens balsamena during the germination period,the effects of two exogenous substances on the seed germination and seedling growth of Impatiens balsamena under NaCl stress were investigated.The seeds of Impatiens balsamena after presoaking treatments with different concentrations of SA (0.5,1.0,1.5,2.0 mmol?L-1) and GB (0.4,0.8,1.2,1.6 mmol?L-1) were cultured adopting a double-layer filter paper method under 100 mmol?L-1 NaCl solution.The results showed that germination rate,germination potential,germination index,vigor index,embryo length,radicle length and fresh weight of seedlings reduced significantly under NaCl stress,and mean germination time was significantly prolonged.The seed germination and seedling growth of Impatiens balsamena were promoted when SA was 1.5 mmol?L-1 and GB were 0.8,1.2 mmol?L-1,while the alleviation effect of NaCl stress reduced,and even aggravated the salt injury when SA and GB concentrations were too high or too low.The comprehensive evaluation using membership function revealed that 1.5 mmol?L-1SA and 0.8 mmol?L-1GB had the satisfactory regulatory effects on seed germination and seedling growth of Impatiens balsamena under NaCl stress,and the effect of 0.8 mmol?L-1 GB was better than 1.5 mmol?L-1 SA.
参考文献/References:
[1]蔺吉祥,高战武,王颖,等.盐碱胁迫对紫花苜蓿种子发芽的协同影响[J].草地学报,2014,22(2):312-318.[2]王楠,高静,黄文静,等.旱、盐胁迫下黄芪种子萌发及其对水杨酸的响应[J].草业科学,2018,35(1):106-114.[3]周杰,黄婷婷,赵光武,等.水杨酸调控种子低温萌发能力的机制研究[J].核农学报,2018,32(8):1649-1655.[4]BOUALLEGUE A,SOUISSI F,NOUAIRI I,et al.Salicylic acid and hydrogen peroxide pretreatments alleviate salt stress in faba bean (Vicia faba) seeds during germination[J].Seed Science and Technology,2017,45(3):1-16.[5]范春丽,曲金柱.甜菜碱浸种对盐胁迫下石榴种子萌发及幼苗生理的影响[J].中国南方果树,2016,45(1):86-90.[6]ANURADHA S,RAO S S R.Effect of brassinosteroids on salinity stress induced inhibition of seed germination and seedling growth of rice (Oryza sativa L.)[J].Plant Growth Regulation,2001,33(2):151-153.[7]彭浩,宋文路,王晓强.水杨酸、脱落酸对盐胁迫下玉米种子萌发和幼苗生长的影响[J].玉米科学,2016,24(6):75-78,87.[8]吴雪霞,查丁石,朱宗文,等.外源2，4-表油菜素内酯对盐胁迫下茄子种子萌发和幼苗生理特性的影响[J].植物生理学报,2011,47(6):607-612.[9]江绪文,李贺勤.外源水杨酸对盐胁迫下广藿香种子萌发和幼苗生长的影响[J].中草药,2015,46(15):2303-2308.[10]LIU J,LI L Y,YUAN F,et al.Exogenous salicylic acid improves the germination of Limonium bicolor seeds under salt stress[J].Plant Signaling and Behavior,2019,14(10):1-8.[11]马婷燕,李彦忠.外源甜菜碱对NaCl胁迫下紫花苜蓿种子萌发及幼苗抗性的影响[J].草业科学,2019,36(12):3100-3110.[12]姜云天,张丽娜,薛飞,等.盐胁迫对茶花凤仙种子萌发的影响[J].东北林业大学学报,2014,42(3):37-41.[13]祖悦.中性盐和碱性盐胁迫对香豌豆和茶花凤仙的影响[D].牡丹江:牡丹江师范学院,2016.[14]李玉梅,田新,姜云天.混合盐胁迫对茶花凤仙种子萌发的影响[J].种子,2019,38(4):36-41,46.[15]张晓军,祖悦,綦嘉莹,等.混合盐碱胁迫对茶花凤仙种子萌发的影响[J].江苏农业科学,2017,45(22):140-143.[16]何会流.外源水杨酸对盐胁迫下凤仙花种子萌发特性的影响[J].北方园艺,2013(13):75-77.[17]梁海英,张雪平,胡志超.Ca2+对盐胁迫下凤仙花种子发芽的影响[J].江苏农业科学,2011,39(6):327-329.[18]何会流.硝普钠对盐胁迫下凤仙花种子萌发及幼苗生理指标的影响[J].西南师范大学学报(自然科学版),2013,38(6):81-84.[19]王财丽,边琦,李婷婷,等.外源Si对NaCl胁迫下肥皂草种子萌发及幼苗生长的影响[J].东北林业大学学报,2016,44(11):41-44.[20]白文华.外源NO和H2S对盐胁迫下苇状羊茅幼苗生理生化的影响[D].泰安:山东农业大学,2018.[21]RAJJOU L,DUVAL M,GALLARDO K,et al.Seed germination and vigor[J].Annual Review of Plant Biology,2012,63(1):507-533.[22]严青青,张巨松,李星星,等.盐碱胁迫对海岛棉种子萌发及幼苗根系生长的影响[J].作物学报,2019,45(1):100-110.[23]袁飞敏,权有娟,陈志国.不同钠盐胁迫对藜麦种子萌发的影响[J].干旱区资源与环境,2018,32(11):182-187.[24]赵宝泉,邢锦城,王静,等.水杨酸对盐胁迫下杭白菊幼苗生长和生理特性的影响[J].吉林农业大学学报,2020,42(4):20-29.[25]单皓,张虎,崔爱民,等.外源生长调节物质对盐胁迫下玉米种子萌发的影响[J].中国农业科技导报,2018,20(8):82-90.[26]JITENDER G.Glycinebetaine and abiotic stress tolerance in plants[J].Plant Signaling and Behavior,2011,6(11):1746-1751.[27]白宝璋,贝丽霞,金锦子.植物生理学[M].北京:中国农业技术出版社,1996.[28]彭立新,李德全,束怀瑞.植物在渗透胁迫下的渗透调节作用[J].天津农业科学,2002,8(1):40-43.[29]于丽丽,曹瑞珍,张学富,等.外源水杨酸对盐胁迫下水飞蓟种子萌发及幼苗生理特性的影响[J].分子植物育种,2019,17(23):7909-7917.[30]黄玉梅,张杨雪,刘庆林,等.水杨酸对盐胁迫下百日草种子萌发及幼苗生理特性的影响[J].草业学报,2015,24(7):97-105.[31]蒋小满,柏新富,赵建萍,等.水杨酸对盐胁迫下三角滨藜种子萌发及幼苗生长的影响[J].中国种业,2007(3):39-40.[32]谷文英,祈新梅,李兴正,等.甜菜碱缓解盐胁迫菊苣种子萌发和芽苗生长的作用[J].中国农学通报,2012,28(29):10-14.[33]王玉萍,董雯,张鑫,等.水杨酸对盐胁迫下花椰菜种子萌发及幼苗生理特性的影响[J].草业学报,2012,21(1):213-219.
相似文献/References:
[1]杨 飞,郭海波,吴 菊,等.NaCl胁迫对白菜种子萌发及幼苗生长的影响[J].北方园艺,2014,38(01):26.
YANG Fei,GUO Hai-bo,WU Ju,et al.Effect of NaCl Stress on the Seed Germination and Seedling Growth of Chinese Cabbage Cultivars[J].Northern Horticulture,2014,38(05):26.
[2]郭艳超,孙昌禹,王文成,等.柳叶马鞭草耐盐性评价研究[J].北方园艺,2014,38(02):79.
GUO Yan- chao,SUN Chang- yu,WANG Wen- cheng,et al.Study on Evaluation of Salt Resistance of Verbena bonariensis [J].Northern Horticulture,2014,38(05):79.
[3]曹玲,刘溪静,李楠茜,等.NaCl胁迫对芹菜种子萌发和幼苗生长的影响[J].北方园艺,2013,37(15):20.
CAO Ling,LIU Xi-jing,LI Nan-xi,et al.Effects of NaCl Stress on Seeds Germination and Seedling Growth of Celery Varieties[J].Northern Horticulture,2013,37(05):20.
[4]闻杰,高志明.NaCl胁迫对紫薇种子萌发和幼苗生长的影响[J].北方园艺,2012,36(02):40.
WEN Jie,GAO Zhi-ming.Effect of Salt Stress on Germination and Seedling Growth of Lagerstroemia indica L.[J].Northern Horticulture,2012,36(05):40.
[5]却志群,沈春修,陈 林.NaCl胁迫对莴苣种子萌发与幼苗生长的影响[J].北方园艺,2013,37(09):12.
QUE Zhi-qun,SHEN Chun-xiu,CHEN Lin.NaCl stress；seed germination；seedling growth[J].Northern Horticulture,2013,37(05):12.
[6]金正律,刘聪聪.氯化钠胁迫与种子大小及其互作对架豆角种子萌发及幼苗生长的影响[J].北方园艺,2013,37(08):39.
JIN Zheng-lv,LIU Cong-cong.Effect of NaCl Stress，Seed Size and Their Interaction on Seed Germination and Seedling Growth of Vigna unguiculata (Linn.) Walp.[J].Northern Horticulture,2013,37(05):39.
[7]刘杰,张建坤,张学政.NaCl胁迫下虎尾草种子萌发特性的研究[J].北方园艺,2013,37(21):92.
LIU Jie,ZHANG Jian-kun,ZHANG Xue-zheng.Study on the Germination Properties of Seeds of Chloris virgate Under NaCl Stress[J].Northern Horticulture,2013,37(05):92.
[8]张弢.氯化钠胁迫对油菜幼苗生理生化指标的影响[J].北方园艺,2012,36(16):16.
ZHANG Tao.Effects of NaCl Stress on Several Physiological and Biochemical Characteristics of Rape Seedlings[J].Northern Horticulture,2012,36(05):16.
[9]吕金海,舒孝顺,刘鹏,等.NaCl胁迫对鱼腥草光合作用的影响[J].北方园艺,2012,36(05):176.
LV Jin-hai,SHU Xiao-shun,LIU Peng,et al.Effects of NaCl Stress on Photosynthesis of Houttuynia cordata Thunb[J].Northern Horticulture,2012,36(05):176.
[10]宋 凯,陆芝傧,冯东方.不同水质对盐胁迫下大蒜种子萌发的影响[J].北方园艺,2012,36(24):32.
SONG Kai,LU Zhi-bin,FENG Dong-fang.The Effect of Garlic Seed Germination for Different Water Quality Under Salt Stress[J].Northern Horticulture,2012,36(05):32.
备注/Memo
第一作者简介：姜云天（1975-），男，硕士，讲师，现主要从事植物逆境生理与生态等研究工作。E-mail：wwwjyt@126.com.责任作者：李玉梅（1976-），女，博士，副研究员，现主要从事植物逆境生理与生态等研究工作。E-mail：mmmlym@126.com.基金项目：吉林省科技发展计划资助项目（20190304012YY）；吉林省大学生创新创业训练计划资助项目（201910202047）；通化师范学院学生创新与实践技能训练资助项目（cs2019041）。收稿日期：2020-03-23