LI Chunling,NIU Chun,ZHANG Ping.Screening and Optimization of Fermentation Conditions of Fermentation Strain for Gibberellin[J].Northern Horticulture,2021,(03):47-52.[doi:10.11937/bfyy.20200962]
赤霉素高产菌株筛选及发酵条件优化
- Title:
- Screening and Optimization of Fermentation Conditions of Fermentation Strain for Gibberellin
- Keywords:
- gibberellin; fermentation strain; Gibberella fujikuroi; solid fermentation; fermentation conditions
- 文献标志码:
- A
- 摘要:
- 以15株产赤霉素的藤仓赤霉菌（Gibberella fujikuroi）为出发菌株，对其脂肪酶活性、制霉素抗性、固态发酵效率进行了比较分析，以期选育出赤霉素发酵生产的优良菌株。结果表明：筛选出2株遗传稳定性好、生产效率高的菌株GA-YJ1704、GA-YJ1715，其发酵效价分别为4.67、4.77 g?kg-1（干基）。该研究结果对赤霉素固态发酵生产的实现具有重要意义。
- Abstract:
- In order to select a good fermentation producing strain of gibberellin，comparative analysis were made in lipase activity，nystatin resistance and production efficiency of 15 strains Gibberella fujikuroi producing gibberellin.The results showed that GA-YJ1704 and GA-YJ1715 strains with high inheritance stability and high fermentation efficiency were screened.The fermentation potency of GA-YJ1704 and GA-YJ1715 strains were 4.67 g?kg-1 and 4.77 g?kg-1 dry basis respectively.The results had great significance for the solid fermentation of gibberellin.
参考文献/References:
[1]ERIKSSON S，BOHLENIUS H，MORITZ T，et al.GA4 is the active gibberellin in the regulation of LEAFY transcription and Arabidopsis floral initiation[J].Plant Cell，2006，18(9):2172-2181.[2]REINECKE D M，WICKRAMARATHNA A D，OZGA J A，et al.Gibberellin 3-oxidase gene expression patterns influence gibberellin biosynthesis，growth，and development in pea[J].Plant Physiology，2013，163(2):929-945.[3]TUDZYNSKI B，MIHLAN M，ROJAS M C，et al.Characterization of the final two genes of the gibberellin biosynthesis gene cluster of Gibberella fujikuroi[J].Journal of Biological Chemistry，2003，278(31):28635-28643.[4]母洪娜，杨秀莲，王良桂.赤霉素在农林业生产中的应用研究进展[J].江苏农业科学，2014，42(2):15-18.[5]彭辉，施天穹，聂志奎，等.微生物发酵产赤霉素的研究进展[J].化工进展，2016,35(11):3611-3618.[6]江澜，马有胜，邱昭辉.赤霉素的固态分批补料发酵与传统的液态发酵比较试验[J].渝州大学学报，1999，16(2):70-72.[7]武文竹，陈俊英，白净，等.赤霉素高产菌株选育及其固态发酵研究进展[J].河南农业科学，2016，45(7):1-6.[8]BEARDER J R，MACMILLAN J，WELS C M，et al.Position of the metabolic block for gibberellin biosynthesis in mutant B1-41a of Gibberella fujikuroi[J].Phytochemistry，1974，13:911-917.[9]CANDAU R，AVALOS J，CERDAA-OLMEDO E.Gibberellins and carotenoids in the wild type and mutants of Gibberella fujikuroi[J].Applied & Environmental Microbiology，1991，7(57):3378-3382.[10]LALE G，JOGDAND V V，GADRE R V.Morphological mutants of Gibberella fujikuroi for enhanced production of gibberellic acid[J].Journal of Applied Microbiology，2006，100:65-72.[11]余卿，覃红，汪育同，等.赤霉素产生菌85104菌株原生质体的形成再生和紫外光对原生质体诱变条件的研究[J].微生物学杂志，1987，6(2):3-7.[12]李武军，郑幼霞.赤霉素生产菌-藤仓赤霉菌融合重组的研究[J].微生物学报，1995，35(4):271-274.[13]张金儿，朱江萍，刘义雄，等.从氯霉素抗性突变株筛选赤霉素高产菌株[J].中国抗生素杂志，2005，3(5):301-303.[14]王卫，李忠海，黎继烈，等.以脂肪酶为指标筛选赤霉素高产菌株的研究[J].工业微生物，2011，41(1):51-54.[15]李祖义，朱明华，冯青，等.胞外脂酶活力的检测法[J].微生物学通报，1990(5):85-88.[16]冯耀基，黎少映，兰红军，等.高效液相色谱法测定蔬菜中的赤霉素GA3、GA4和GA7[J].微量元素与健康研究，2017，34(3):44-46.[17]王卫，郭珺，曾柏全，等.利用制霉菌素抗性筛选赤霉素高产菌株的研究[J].北方园艺，2011(23):121-123.[18]廖海兵，雷文，徐永华.利用制霉菌素抗性筛选赤霉素高产菌株[J].农业科学，2015，35(2):1.[19]刘风莉，白净，李攀，等.赤霉素固态发酵培养基的研究[J].广东化工，2018，45(8):38.[20]CORONA A，SANCHEZ D，AGOSTIN E.Effect of water activity on gibberellic acid production by Gibberella fujikuroi under solidstate fermentation conditions[J].Process Biochemistry，2005，40:2655-2658.[21]王卫，黎继烈，黄卫文，等.利用特比萘芬抗性筛选赤霉素高产菌株及相关发酵特性的研究[J].微生物学通报，2014，41(9):1837-1842.
相似文献/References:
[1]梁 芳,衣采洁.不同浸种处理对小冠花及蛇鞭菊种子萌发的影响[J].北方园艺,2014,38(11):62.
LIANG Fang,YI Cai-jie.Infulence of Different Processing Methods on the Seed Germination of Coronilla varia and Liatris spieata [J].Northern Horticulture,2014,38(03):62.
[2]郑 晟,邓 赞,张凌云.大白杜鹃种子无菌萌发及幼苗生长条件研究[J].北方园艺,2014,38(11):77.
ZHENG Sheng,DENG Zan,ZHANG Ling-yun.Research on Seeds Aseptic Germination and Seedling Growth Condition of Rhododendron decorum Franch[J].Northern Horticulture,2014,38(03):77.
[3]马孟莉,刘艳红,张建华,等.外源赤霉素对仙客来开花的影响[J].北方园艺,2013,37(22):89.
MA Meng-li,LIU Yan-hong,ZHANG Jian-hua,et al.Effect of the Exogenous Gibberellin on Cyclamen persicum Bloom[J].Northern Horticulture,2013,37(03):89.
[4]李德明,陈平,张秀娟,等.赤霉素及硫酸铵对低温条件下榕树幼株生理生化指标的影响[J].北方园艺,2014,38(04):53.
LI De-ming,CHEN Ping,ZHANG Xiu-juan,et al.Effect of Gibberellic Acid and Ammonium Sulfate on Physiological and Biochemical Idexes of Ficus microcorpa Plant Under Low Temperature Stress[J].Northern Horticulture,2014,38(03):53.
[5]钱瑭璜,梁琼芳,雷江丽.三种新优野生地被植物的繁殖技术研究[J].北方园艺,2013,37(13):84.
QIAN Tang-huang,LIANG Qiong-fang,LEI Jiang-li.Research on the Propagation Technology of Three New Wild Ground Cover Plants[J].Northern Horticulture,2013,37(03):84.
[6]杨国会,孙立梅,邢力,等.不同层积处理方法对东北红豆杉种子后熟过程中贮藏物质的影响[J].北方园艺,2013,37(14):82.
YANG Guohui,SUN Limei,XING Li,et al.Effects of Different Concentrations of GA3 with Different Temperature Stratifications on the Storage Substance Changes of Taxus cuspidata Sieb.et Zucc.[J].Northern Horticulture,2013,37(03):82.
[7]徐巍,周艳,袁蕊,等.赤霉素处理对不结球白菜现蕾开花的影响[J].北方园艺,2013,37(03):13.
XU Wei,ZHOU Yan,YUAN Rui,et al.Effects of GA3 on Budding and Flowering of Non-heading Chinese Cabbage[J].Northern Horticulture,2013,37(03):13.
[8]胡亚岚,王晓玲,毛永民.不同喷施物对婆枣裂果指标及果实内在品质的影响[J].北方园艺,2013,37(21):30.
HU Ya-lan,WANG Xiao-ling,MAO Yong-min.Effect of Different Spraying Agents on Index of Fruit Cracking and Interior Quality of Pozao Jujube[J].Northern Horticulture,2013,37(03):30.
[9]孙敏杰,吴媛媛.易丰收与赤霉素对切花洋桔梗生长发育的研究[J].北方园艺,2012,36(15):89.
SUN Min-jie,WU Yuan-yuan.EFS and Gibberellin on Growth and Development of Cut Flower Eustoma[J].Northern Horticulture,2012,36(03):89.
[10]李然红,于丽杰,安文和,等.银杏种子繁殖技术研究[J].北方园艺,2012,36(17):151.
LI Ran-hong,YU Li-jie,AN Wen-he,et al.Study on Seminal Propagation Technology of Ginkgo biloba L.[J].Northern Horticulture,2012,36(03):151.
备注/Memo
第一作者简介：李春玲(1982-)，女，本科，助理研究员，现主要从事微生物发酵菌种选育等研究工作。E-mail：lichunling_@163.com.责任作者：张萍(1977-)，女，本科，工程师，现主要从事微生物发酵等研究工作。E-mail：18995018336@163.com.收稿日期：2020-03-15