SUN Xieping,ZHANG Xin,LUO Youjin,et al.Effects of Four Valence State of Selenium on Part of Root Physiological Characteristics of Plum Seedlings[J].Northern Horticulture,2021,(03):41-46.[doi:10.11937/bfyy.20202157]
四种价态硒对“青脆”李幼苗根系部分生理特性的影响
- Title:
- Effects of Four Valence State of Selenium on Part of Root Physiological Characteristics of Plum Seedlings
- 文献标志码:
- A
- 摘要:
- 以“青脆”李为试验材料，根施4种价态的硒，研究其对李子幼苗生长、根系谷胱甘肽氧化循环以及抗氧化系统的影响，以期为富硒李发展提供参考依据。结果表明：不同价态硒对幼苗生长产生不同的影响，其中Se6+和Se4+显著促进了株高和直径。4种价态硒处理显著提高了根系丙二醛（MDA）含量，也显著提高了过氧化氢酶（CAT）和谷胱甘肽转移酶（GST)活性。Se6+处理下根系总抗氧化能力（AOC ）最高，其次为Se0，Se2-和Se4+最低。4种价态硒均显著提高了根系还原型谷胱甘肽(GSH)、氧化型谷胱甘肽（GSSG）和GSH+GSSG含量、GSH/GSSG比值，以及谷胱甘肽还原酶（GR）和谷胱甘肽过氧化物酶（GPX）活性，但4种价态硒处理间未存在显著水平。总之，硒对根系GSH氧化循环有促进作用，但不存在明显的价态差异，而不同价态硒对根系抗氧化系统和幼苗生长存在价态上差异。Se6+在促进生长和抗氧化能力表现较好。
- Abstract:
- Taking ‘Qingcui’ plum as test materials,four forms of Se were applied to the roots of the ‘Qingcui’ plum seedlings.The effects of Se forms on the growth，glutathione oxidation cycle and antioxidant system of plum seedlings were studied.In order to provide reference for plum development.The results showed that different forms of selenium had different effects on the growth of seedlings，and Se6+ and Se4+ significantly promoted plant height and stem diameter.Four forms of Se treatment significantly increased the content of MDA，consistent with significantly increased activities of CAT and GST.The highest AOC was in Se6+ treatment，followed by Se0，the lowest Se2- and Se4+.The contents of GSH，GSSG，and GSH+GSSG，the ratio of GSH-GSSG，and the activity of GR and GPX were significantly increased by the four forms of Se treatment.But there was no significant difference among the four forms of Se treatment.All in all，Se can significantly promote the GSH oxidation cycle，without form difference，while different forms of Se have morphological differences in antioxidant system and growth parameters.Se6+ showed better growth promotion and antioxidant capacity.
参考文献/References:
[1]王武，尹旭敏，李勋兰，等.重庆地区李品种概述及产业现状[J].中国南方果树，2019，48(6):164-170.[2]FENG R，WEI C，TU S.The roles of selenium in protecting plants against abiotic stresses[J].Environmental and Experimental Botany，2013，87（1）:58-68.[3]EICH-GREATOREX S，SOGN T A，GAARD A F，et al.Plant availability of inorganic and organic selenium fertilizer as influenced by soil organic matter content and pH[J].Nutrient Cycling in Agroecosystems，2007，79(3):221-231.[4]NOWAK J，KAKLEWSKI K，LIGOCKI M.Influence of selenium on oxidoreductive enzymes activity in soil and in plants[J].Soil Biology and Biochemistry，2004，36(10):1553-1558.[5]BAUELOS G S，LIN Z Q.Phytoremediation management of selenium-laden drainage sediments in the San Luis Drain:A greenhouse feasibility study[J].Ecotoxicology and Environmental Safety，2005，62(3):309-316.[6]代涛,尹志峰,王良友.还原型谷胱甘肽临床应用研究进展[J].承德医学院学报，2014，31(5):432-435.[7]卢佃华.谷胱甘肽的药理作用及研究新进展[J].中国医药指南，2013，11(29):40-41.[8]孙协平，罗友进，周广文.硒对甜樱桃叶片褪黑素和谷胱甘肽氧化还原循环的影响[J].中国农业科学，2017，50(22):4373-4381.[9]罗红艳，石零珊，汪凤林,等.低磷和铝毒耦合胁迫对杉木叶片抗坏血酸:谷胱甘肽循环的影响[J].热带作物学报，2018，39(5):873-880.[10]KANWAR M K，XIE D，YANG C，et al.Melatonin promotes metabolism of bisphenol A by enhancing glutathione-dependent detoxification in Solanum lycopersicum L[J].Journal of Hazardous Materials，2020，388：121727.[11]PUCCINELLI M，MALORGIO F,PEZZAROSSA B.Selenium enrichment of horticultural crops[J].Molecules，2017，22（6）:933.[12]杨建.水稻根系形态生理与产量、品质形成及养分吸收利用的关系[J].中国农业科学，2011，44(1):36-46.[13]沈荣明.硒的营养功能及富硒产品的开发前景[J].粮食流通技术，2016(14):24-25.[14]吴露露，杨安富，耿建梅，等.硒对不同类型杂交水稻品种发芽特性的影响[J].热带作物学报，2010，31(5):711-718.[15]FILEK M，GZYL-MALCHER B，ZEMBALA M，et al.Effect of selenium on characteristics of rape chloroplasts modified by cadmium[J].Journal of Plant Physiology，2010，167(1):28-33.[16]SUN X，WANG Y，HAN G，et al.Effects of different selenium forms on selenium accumulation，plant growth，and physiological parameters of wild peach[J].South African Journal of Botany，2020，131:437-442.[17]周健，郝苗，刘永红，等.不同价态硒缓解小油菜镉胁迫的生理机制[J].植物营养与肥料学报，2017，23(2):444-450.[18]孙协平，罗友进，周广文，等.硒的价态与浓度对香橙幼苗生长和AsA-GSH循环的影响[J].应用生态学报，2017，28(11):3527-3534.[19]闫金朋.施用不同形态硒对镉胁迫下水稻生长及吸收、转运镉的影响[D].南宁：广西大学，2019.[20]HAGHIGHI M，SHEIBANIRAD A，PESSARAKLI M.Effects of selenium as a beneficial element on growth and photosynthetic attributes of greenhouse cucumber[J].Journal of Plant Nutrition，2016，39(10):1493-1498.[21]CARMAGNOL F，SINET P M，JEROME H.Selenium-dependent and non-selenium-dependent glutathione peroxidases in human tissue extracts[J].Biochimicaet Biophysica Acta (BBA)-General Subjects，1983，759(1-2):49-57.[22]TOLFA I，VELKI M，VUKOVIC〖JX-+0.8mm〗〖KG-*2〗＇ 〖KG*2〗 R，et al.Effect of different forms of selenium on the plant-soil-earthworm system[J].Journal of Plant Nutrition and Soil Science，2017，180（2）:231-240.[23]CARTES P，GIANFREDA L，MORA M L.Uptake of selenium and its antioxidant activity inryegrass when applied as selenate and selenite forms[J].Plant and Soil，2005，276(1/2):359-367.[24]SEPPNEN M，TURAKAINEN M，HARTIKAINENH.Selenium effects on oxidative stress in potato[J].Plant Science (Oxford)，2003，165（2）:311-319.[25]赵耀，吴珍龄，杨盛亚.硒酸钠对小麦幼苗中谷胱甘肽过氧化物酶和谷胱甘肽转硫酶活性以及谷胱甘肽含量的影响[J].植物生理学通讯，2004，40(2):178-180.[26]周文美.硒对水稻生化活性的影响[J].山地农业生物学报，1996，17(6):323-326.[27]KAPOOR R，SINGH N.Arbuscular mycorrhiza and reactive oxygen species.In:Wu Q.S.(Ed.)，arbuscular mycorrhizas and stress tolerance of plants[M].Singapore：Springer Nature Singapore Pte Ltd，2017.[28]GOLKAR P，ARZANI A，REZAEI A M，et al.Genetic variation of leaf antioxidants and chlorophyll content in safflower[J].African Journal of Agricultural Research，2009，4（12）:1475-1482.[29]赵凤云，宋新华.硒和谷胱甘肽复合作用对大麦苗生理和抗氧化特性的影响[J]．江苏农业科学，2019，47(21):120-123.[30]KUMAR S，ASIF M H，CHAKRABARTY D，et al.Differential expression of rice lambda class GST gene family members during plant growth，development，and in response to stress conditions[J].Plant Molecular Biology Reporter，2013，31:569-580.[31]LI C，ZHENG Y，ZHOU J，et al.Changes of leaf antioxidant system,photosynthesis and ultrastructure in tea plant under the stress of fluorine[J].Biologia Plantarum (Prague)，2011，55(3)：563-566.[32]AKBUDAK M A，FILIZ E，VATANSEVER R，et al.Genome-wide identification and expression profiling of ascorbate peroxidase (APX) and glutathione peroxidase (GPX) genes under drought stress in sorghum (Sorghum bicolor L.)[J].Journal of Plant Growth Regulation，2018,37（3）:925-936.[33]贾玮，吴隽，屈婵娟,等.硒增强植物抗逆能力及其机理研究进展[J].中国农学通报，2015，31(14):171-176.
备注/Memo
第一作者简介：孙协平（1983-），女，博士，讲师，现主要从事果树生理与生态等研究工作。E-mail：xieping444@163.com.责任作者：周先容（1968-），男，硕士，教授，现主要从事植物资源与植物生态学等研究工作。E-mail：zxrfsy@163.com.基金项目：2018年重庆市基础研究与前沿探索资助项目(cstc2018jcyjAX0618)；国家自然科学基金资助项目(31901972)。收稿日期：2020-05-27