XU Chenjie,OU Jing.Comparative Stem Culture and Embryo Culture of Cerasus yedoensis ‘Somei-yoshino’[J].Northern Horticulture,2020,44(23):65-71.[doi:10.11937/bfyy.20200224]
染井吉野樱的茎段培养与胚培养比较
- Title:
- Comparative Stem Culture and Embryo Culture of Cerasus yedoensis ‘Somei-yoshino’
- Keywords:
- Cerasus yedoensis ‘Somei-yoshino’; stem segment; embryo culture; cluster buds; growth regulator
- 文献标志码:
- A
- 摘要:
- 以染井吉野樱为试材，比较茎段及种胚2种外植体在启动及增殖阶段的表现，探讨2种外植体的成苗效率以及不同植物生长调节剂对染井吉野樱芽诱导、不定芽增殖及生根的影响。结果表明：在MS+6-BA 2.0 mg?L-1+IBA 0.1 mg?L-1中腋芽诱导效果最好，诱导率可达61.29%。NAA对胚芽诱导的影响不显著，在MS+6-BA 2.0 mg?L-1+IBA 0.05 mg?L-1+NAA 0.05 mg?L-1中种胚的诱导率可达86.02%。适宜的增殖培养基为MS+6-BA 2.0 mg?L-1+IBA 0.1 mg?L-1，腋芽苗及胚芽苗的平均增殖系数分别达到了3.73和4.09。在1/2MS+IBA 0.5 mg?L-1+NAA 0.1 mg?L-1中生根率可达70.97%。生根后的幼苗经过炼苗，移栽在腐殖质∶河沙∶蛭石体积比为2∶1∶1的基质中，成活率可达87.09%。
- Abstract:
- Cerasus yedoensis‘Somei-yoshino’ was used as material,seeding efficiency of different explant by comparing the induction rate of stem with axillary bud and embryo which used as explant in single bud induction,cluster buds induction was imestigate.And the effect of different plant growth regulators on single bud induction，cluster buds induction and rotting was explored.The results showed that MS medium supplemented with 2.0 mg?L-1 6-BA and 0.1 mg?L-1 IBA showed the highest single bud induction rate (61.29%)using stem with axillary bud as explants.The influence of NAA for embryo induction was not significant.MS medium supplemented with 2.0 mg?L-1 6-BA，0.05 mg?L-1 IBA and 0.05 mg?L-1 NAA showed the highest single bud induction rate (86.02%)using embryo as explants．MS medium with 2.0 mg?L-1 6-BA and 0.1 mg?L-1 IBA exhibited the highest clustered bud proliferation rate (3.73 and 4.09)．1/2MS medium with 0.5 mg?L-1 IBA and 0.1 mg?L-1 NAA was suitable for plantlet rooting,and the rooting rate was 70.97%.The survive rate was above 87.09% when the plantlets were due to be moved to the nursery.The medium with 50% humus,25% sand and 25% roseite was effective for rooting．
参考文献/References:
[1]李科,杨广军,潘新军,等.染井吉野樱在洞庭湖区的引种栽培试验[J].湖南林业科技,2017,44(4):89-91,102.[2]游雪萍.染井吉野樱在园林绿化中的应用[J].山西林业,2019(2):42-43.[3]许早时.染井吉野樱组织培养与快繁研究[J].园艺与种苗,2018,38(1):31-33.[4]吴思政,聂东伶,柏文富.染井吉野樱花扦插繁殖技术研究[J].湖南林业科技,2012,39(6):11-14,27.[5]陈秀云.染井吉野樱的栽培技术[J].现代园艺,2013(15):30-31.[6]姚连芳,张建伟,冷天波,等.樱花组培快繁生产技术[J].林业实用技术,2004(3):27-28.[7]吴丽芳,魏晓梅,陆伟东.白刺花胚性愈伤组织诱导及体细胞胚发生[J].林业科学,2019,55(7):170-177.[8]曹昆,李霞.木本植物组织培养不定芽诱导研究进展[J].江苏林业科技,2008(5):43-48.[9]邹娜,陈璋,林思祖,等.福建山樱花愈伤组织的诱导及植株再生[J].核农学报,2013,27(10):1417-1423.[10]南程慧.迎春樱居群变异与繁殖生物学研究[D].南京:南京林业大学,2012.[11]张帆,叶露,董姬妃,等.高盆樱桃的组培快繁研究[J].南京林业大学学报(自然科学版),2018,42(3):111-116.[12]李明,赵改荣,刘聪利,等.早熟欧洲甜樱桃高效胚培养成苗技术研究[J].果树学报,2014,31:70-73.[13]闫道良.钟花樱群落特征与繁殖技术研究[D].南京:南京林业大学,2005.[14]徐世彦,武凯翔,吴延军.樱桃组织培养及遗传转化研究进展[J].果树学报,2018(10):1277-1285.[15]夏明霞,张宁宁,江兴瑜,等.樱花品种‘染井吉野’启动培养初步研究[J].金陵科技学院学报,2018(3):78-80.[16]荣冬青,王贤荣.垂枝早樱‘红枝垂’组培快繁试验[J].林业工程学报,2008(5):72-75.[17]朱育端.东京樱花组培快繁技术研究[J].林业勘察设计,2012(1):158-160.[18]黄守印,池井存,苏淑欣,等.雾灵山地区野生樱花的组织培养与快速繁殖[J].植物生理学报，2003,39(3):228.[19]邹娜,曹光球,林思祖.观赏樱花繁殖技术研究进展[J].西南林学院学报,2007(6):42-46.
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备注/Memo
第一作者简介：徐晨捷（1995-），男，硕士研究生，研究方向为园林植物繁殖及容器育苗。E-mail:770904368@qq.com.责任作者：欧静（1974-），女，博士，教授，硕士生导师，现主要从事植物资源及应用等研究工作。E-mail:coloroj@126.com.基金项目：贵州省农业科技支撑资助项目(黔科合支撑[2018]2287号)。收稿日期：2020-02-24