FU Zhenzhu,YUAN Xin,WANG Aike,et al.Tissue Culture of Colored Tree Cultivar Melia azedarach[J].Northern Horticulture,2020,44(13):71-76.[doi:10.11937/bfyy.20192882]
彩叶楝树组织培养技术
- Title:
- Tissue Culture of Colored Tree Cultivar Melia azedarach
- Keywords:
- colored tree; Melia azedarach; plant hormones; tissue culture
- 文献标志码:
- A
- 摘要:
- 以彩叶楝树新品种‘美人’当年生幼嫩茎段为试材，采用组织培养方法，研究了不同浓度的6-BA、IBA及NAA对彩叶楝树组培快繁的影响，以期建立彩叶楝树的高效组培快繁体系。结果表明：外植体经1 ｇ?Ｌ-1的多菌灵消毒10 min和300 ｍg?Ｌ-1的羧苄青霉素消毒5 min后，再用0.2%氯化汞消毒15 min，灭菌效果较好，污染率只有32%。不定芽增殖的适宜培养基为MS+6-BA 0.3 ｍg?Ｌ-1+IBA 0.20 ｍｇ?Ｌ-1，增殖系数为4.76；而1/2MS+IBA 0.10 ｍｇ?Ｌ-1+NAA 0.05 ｍｇ?Ｌ-1为试管苗生根较理想的配方，生根率可达90.8%；试管苗移栽适宜的基质为草碳∶珍珠岩=2∶1，成活率可达90%。该研究建立了一套完善的彩叶楝树新品种组培快繁体系，对其种植资源保护、新品种繁育、推广及遗传改良有着重要的理论指导意义和实践应用价值。
- Abstract:
- The annual stem segments of the new colored tree cultivar Melia azedarach‘Meiren’ were used as experimental material to study the effect of different concentrations of 6-BA,IBA and NAA on its tissue culture so that to establish an efficient rapid propagation system of tissue culture for Melia azedarach‘Meiren’.The results showed that the explants were disinfect with 0.2% mercury for 15 minutes after sterilizing for 10 minutes by 1 g?Ｌ-1 carbendazim and 5 minutes by 300 ｍｇ?Ｌ-1carboxy benzyl.The contamination rate was only 32%.The suitable culture medium for adventitious bud proliferation was MS+6-BA 0.3 ｍｇ?Ｌ-1+IBA 0.20 ｍｇ?Ｌ-1 with the proliferation coefficient was 4.76.While 1/2MS+IBA 0.10 ｍｇ?Ｌ-1+NAA 0.05 ｍｇ?Ｌ-1 was the ideal culture medium for rooting.The rooting rate could reach 90.8%.The survival rate was 90% when the plantlets were transplanted in the substrate of grass carbon∶perlite=2∶1.This study established a set of perfect rapid propagation systemof tissue culture for the new colored tree cultivar of Melia azedarach，which had the important theoretical and practical significance to the protection of germplasm resources，propagation，spreading and genetic improvement of the new cultivar.
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备注/Memo
第一作者简介：符真珠（1982-），女，博士，助理研究员，现主要从事园林植物生物技术育种等研究工作。E-mail:pearlgh2005@163.com.责任作者：张和臣（1979-），男，博士，副研究员，现主要从事园林植物分子生物学等研究工作。E-mail:zhc5128@126.com.基金项目：河南省农业科学院自主创新基金资助项目（2017ZC25）。收稿日期：2019-09-20