[1]丰美静,张恺恺,黄中文,等.东北红豆杉温室扦插繁殖试验[J].北方园艺,2020,44(13):65-70.[doi:10.11937/bfyy.20192998]
　FENG Meijing,ZHANG Kaikai,HUANG Zhongwen,et al.Experiment of Cuttage Propagation of Taxus cuspidata in Greenhouse[J].Northern Horticulture,2020,44(13):65-70.[doi:10.11937/bfyy.20192998]
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东北红豆杉温室扦插繁殖试验

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第44卷 期数: 2020年13 页码: 65-70 栏目: 出版日期: 2020-07-15
Title:
Experiment of Cuttage Propagation of Taxus cuspidata in Greenhouse
作者:
丰美静1; 2; 张恺恺1; 黄中文3; 邱德有2; 陈段芬1; 杨艳芳2
（1.河北农业大学 园艺学院，河北 保定 071001；2.中国林业科学研究院 林业研究所，林木遗传育种国家重点实验室，国家林业局林木培育重点实验室，北京 100091；3.河南科技学院 生命科技学院，河南 新乡 453003）
Author(s):
FENG Meijing1; 2; ZHANG Kaikai1; HUANG Zhongwen3; QIU Deyou2; CHEN Duanfen1; YANG Yanfang2
(1.College of Horticulture,Agricultural University of Hebei,Baoding,Hebei 071001；2.Research Institute of Forestry,Chinese Academy of Forestry/State Key Laboratory of Tree Genetics and Breeding/Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration,Beijing 100091；3.Institute of Science and Technology,Henan Collaborative Innovation Center of Modern Biological Breeding,Xinxiang,Henan 453003)
关键词:
红豆杉; ABT1; 扦插; 生根率; 温室
Keywords:
Taxus L.; ABT1; cutting; rooting rate; greenhouse
DOI:
10.11937/bfyy.20192998
文献标志码:
A
摘要:
以不同品种的红豆杉枝条为试材，利用L9（34）正交实验方法，探讨了在温室环境条件下，东北红豆杉不同插穗年龄、不同浓度ABT1以及不同浸泡时间对扦插繁殖生根率的影响，并确定了通用于其它种红豆杉扦插繁殖的最佳试验方案。结果表明：3种因素中插穗年龄和浸泡时间显著影响东北红豆杉生根率，而ABT1的浓度对东北红豆杉生根率不存在显著作用。此外，方案组合A1B3C3生根率最高，而A3B1C3生根率与之较为接近，即同为一年生枝条（C3），在较低浓度ABT1（A1：50 mg?L-1）处理较长时间（B3：4.0 h）和在较高浓度ABT1(A3：500 mg?L-1)处理较短时间（B1：0.5 h）均能够得到较高生根率（96%左右），表明较低浓度ABT1浸泡时间稍长，较高浓度ABT1浸泡时间稍短，有利于插穗生根。将A1B3C3组合在南方红豆杉和曼地亚红豆杉中进行验证，2种红豆杉扦插生根率均达到90%以上。该研究所建立的温室内扦插繁殖技术方案，简便易行，可以为其它种红豆杉扦插繁育所借鉴。
Abstract:
Different Taxus branches were selected as test materials,using the L9(34) orthogonal test method,9 combinations of cuttage propagation of Taxus cuspidata were carried out in greenhouse.Each combination was repeated 3 times with 50 cuttings,and the rooting rate was investigated and analyzed.The effects of the age of cuttings,the concentration of ABT1,and the length of soaking time on the rooting rate were analyzed and discussed.Moreover,the best combination for cuttings propagation of yew was obtained.The results showed that the rooting rate of T.cuspidata was significantly influenced by the age of cuttings and the length of soaking time,while the concentration of ABT1 had no significant effect.In addition,the program A1B3C3 had the highest rooting rate,and the rooting rate of A3B1C3 was nearly to A1B3C3.In details,the same one-year branch (C3),higher rooting rate could be obtained at lower concentration ABT1 (A1,50 mg?L-1) for longer time (B3,4.0 hours) or at higher concentration ABT1 (A3,500 mg?L-1) for shorter time (B1,0.5 hour) (rooting rate about 96%).The results indicated that with lower concentration ABT1,the treating time was longer,while with higher concentration ABT1,the treating time was shorter.Moreover,the combination A1B3C3 was used in Taxus chinensis var.mairei and Taxus×media,and the rooting rates of both yew reached more than 90%.The technology of cuttage propagation established by this research was simple and easy to use,and could be used for reference for other species of yew cuttage breeding in greenhouse.

参考文献/References:

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备注/Memo

第一作者简介：丰美静（1994-），女，硕士研究生，研究方向为植物种质资源创新与利用。E-mail:fengmeijing@163.com.责任作者：杨艳芳（1978-），女，博士，副研究员，现主要从事林木次生代谢与调控等研究工作。E-mail:echoyyf@caf.ac.cn.基金项目：“十三五”国家重点研发计划林业资源培育及高效利用技术创新重点专项资助项目(2017YFD0600706)；国家自然科学基金资助项目(31570675)；中国林业科学研究院基本科研业务费专项资金资助项目(CAFYBB2018SY009)。收稿日期：2019-11-14

更新日期/Last Update: 2020-09-09