ZHENG Hanyu,JIN Heyan,FENG Yichuan,et al.Effects of Nitrogen and Phosphorus on the Accumulation of Arbutin in Adventitious Roots of Astragalus membranaceus[J].Northern Horticulture,2020,44(07):137-141.[doi:10.11937/bfyy.20192687]
氮和磷对膜荚黄芪不定根芒柄花苷积累的影响
- Title:
- Effects of Nitrogen and Phosphorus on the Accumulation of Arbutin in Adventitious Roots of Astragalus membranaceus
- Keywords:
- Astragalus membranaceus; adventitious roots; ononin; nitrogen; phosphate
- 文献标志码:
- A
- 摘要:
- 以膜荚黄芪不定根为试材，通过改变B5培养基成分，依次研究了总氮含量、NH+4∶NO-3比例、磷酸盐含量对不定根生物量以及芒柄花苷积累的影响，以期得到最佳氮、磷配比。结果表明：当培养基中总氮浓度为3.0 mmol·L-1、NH+4∶NO-3比例为1.5∶1.5、PO3-4浓度为0.625 mmol·L-1时，黄芪不定根生物量及芒柄花苷含量均依次达到了最大值，且与初始培养基相比芒柄花苷的含量分别增加了7、14、19倍。因此，该方法简单可靠，且配比结果为进一步利用膜荚黄芪不定根规模化生产芒柄花苷奠定了理论基础。
- Abstract:
- Astragalus membranaceus adventitious roots (AMARs) were used as the test material,the effects of total nitrogen content,NH+4∶NO-3 ratio and phosphate content on the biomass of adventitious roots and the accumulation of anthocyanin were studied by changing the composition of B5 medium.In order to get the best ratio of nitrogen and phosphorus.The results showed that when the total nitrogen concentration was 3.0 mmol·L-1,NH+4∶NO-3 ratio was 1.5∶1.5,PO3-4 concentration was 0.625 mmol·L-1,both biomass and content of ononin in AMARs reached the maximum,and each ononin content increased about 7,14,19 times compared with the initial medium,respectively.Therefore,the method was simple and reliable,and the results of proportioning lay a theoretical foundation for further production of anthocyanin in AMARs.
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备注/Memo
第一作者简介：郑涵予（1992-），女，硕士研究生，研究方向为特种植物资源与生物技术。E-mail:824222431@qq.com.责任作者：吴松权（1972-），男，博士，副教授，研究方向为特种植物资源与生物技术。E-mail:arswsq@ybu.edu.cn.基金项目：国家自然科学基金资助项目（21462044，30860036）；吉林省自然科学基金资助项目（201115228）。收稿日期：2019-09-19