CHEN Quanyin,XU Zongyi,WANG Yue,et al.Effect of NaCl and PEG Iso-osmotic Stress on Photosystem Ⅱ in Mulbery Leaves[J].Northern Horticulture,2020,44(07):92-100.[doi:10.11937/bfyy.20192539]
NaCl和等渗PEG胁迫对桑树叶片光系统Ⅱ的影响
- Title:
- Effect of NaCl and PEG Iso-osmotic Stress on Photosystem Ⅱ in Mulbery Leaves
- Keywords:
- Morus alba; NaCl stress; PEG stress; D1 protein turnover; xanthophylls cycle; PSⅡ
- 文献标志码:
- A
- 摘要:
- 以桑树品种“秋雨桑”为试材，利用植物叶绿素荧光动力学技术，研究了等渗NaCl和PEG胁迫对桑树叶片光合作用的光系统Ⅱ（PSⅡ）的影响。结果表明：与对照（CK）比较，桑树叶片在150 mmol·L-1 NaCl和等渗17.1% PEG胁迫下均保持较高的PSⅡ反应中心活性，具有完善的光防御保护机制。寻找NaCl和等渗PEG对桑树叶片PSⅡ反应中心的伤害部位，发现4种抑制剂对2种胁迫条件下桑树叶片的光合作用位点不同，盐胁迫主要通过叶黄素循环途径，以及维持QA、QB间电子的正常传递来保护PSⅡ反应中心活性，而PEG胁迫下D1蛋白周转减弱伤害。
- Abstract:
- Taking mulberry (Morus alba L.) as the experimental material,the effect of mulberry leaves on photosystem Ⅱ (PSⅡ) photoinhibition under salt stress and isotonic PEG stress was investigated by utilizing inhibitor.The results showed that compared with CK,mulberry leaves maintained higher PSⅡ reaction center activity under 150 mmol·L-1 NaCl and isotonic 17.1% PEG stress,and had perfect light damage protection mechanism.Under the simulation of salt and isotonic drought stress,the damage of mulberry leaf which happened in PSⅡ reaction centers by utilizing inhibitor.The photosynthetic sites of mulberry leaves were different using two kinds of stress conditions after under inhibitors.Salt stress mainly protected PSⅡ reaction center activity through lutein cycle pathway and normal electron transfer between QA and QB,while D1 protein turnover played a greater role under PEG stress.
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备注/Memo
第一作者简介：陈泉印（1998-），男，辽宁盖州人，硕士研究生，研究方向为植物生理生态。E-mail:chenquanyin98@163.com.责任作者：孙广玉（1963-），男，黑龙江巴彦人，博士，教授，现主要从事植物生理学等研究工作。E-mail:sungy@nefu.edu.cn.基金项目：国家自然科学基金资助项目（31870373）。收稿日期：2019-08-30